LOCUS       sequence-396477-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mitochondrial human malate dehydrogenase 2 
            (hMDH2) transcript variant 1; Use in CURES (integration of research 
            into undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET28a hMDH2
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             946..966
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             967..984
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctaaag tagctgttct gggtgcatct ggtggtattg gtcaaccgct gtccctgctg
       61 ctgaagaact ctccactggt tagccgtctg accctgtacg atatcgcaca tactccaggc
      121 gttgctgctg acctgtccca cattgagact aaagctgcgg tgaaaggcta cctgggtcca
      181 gaacagctgc cagattgtct gaaaggttgt gacgtggtag ttatcccagc aggtgttcca
      241 cgtaaaccag gtatgacccg tgatgacctg ttcaatacta atgcgactat cgttgcgacc
      301 ctgaccgctg cttgcgctca gcactgtccg gaagctatga tctgtgtgat tgctaatccg
      361 gttaacagca ccatcccgat tactgccgag gtattcaaga aacacggtgt ttacaacccg
      421 aacaagatct tcggtgttac caccctggat atcgtgcgtg caaacacctt tgtggctgaa
      481 ctgaaaggtc tggatccagc tcgcgtaaac gttccggtaa tcggtggtca cgctggcaag
      541 accatcattc cgctgatttc ccagtgcact ccgaaagtag acttcccaca ggaccagctg
      601 accgctctga ctggtcgtat ccaggaggca ggcactgaag tggtgaaagc gaaagcaggt
      661 gctggctctg cgactctgag catggcctac gctggtgctc gtttcgtatt ctctctggtt
      721 gacgcaatga acggcaagga aggtgtagtg gaatgttcct tcgtgaagtc tcaagaaacc
      781 gaatgcacct acttcagcac tccactgctg ctgggcaaga aaggcatcga gaagaacctg
      841 ggcatcggta aagtttcttc tttcgaagag aagatgatct ccgacgcaat tccggaactg
      901 aaagcgagca tcaagaaggg tgaagatttc gtgaagaccc tgaaagagaa cctgtacttc
      961 cagggtcatc atcaccacca tcactaa
//
