LOCUS       sequence-396479-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of yeast mitochondrial malate dehydrogenase; 
            Use in CURES (integration of research into undergraduate teaching 
            labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET28a MDHM_YEAST
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             955..975
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             976..993
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtacaagg ttactgtact gggtgcaggt ggtggcattg gtcaaccgct gtccctgctg
       61 ctgaagctga accacaaagt taccgacctg cgtctgtacg acctgaaagg tgctaaaggt
      121 gtggctaccg acctgagcca catcccgact aactctgttg tgaaaggctt tactccggaa
      181 gaaccggatg gcctgaacaa cgctctgaag gataccgata tggttctgat tccagctggt
      241 gttccacgta aaccaggtat gactcgtgac gatctgttcg ccatcaacgc atccatcgta
      301 cgcgacctgg ctgctgcaac cgcagaaagc gcaccaaacg cagctatcct ggtgatcagc
      361 aatccagtaa actctactgt tccgatcgtg gctcaggtac tgaagaacaa aggtgtgtac
      421 aacccgaaga agctgtttgg cgttactacc ctggacagca tccgtgctgc acgcttcatt
      481 tccgaagtgg agaacaccga tccgacccag gaacgtgtga atgtaatcgg tggtcactct
      541 ggcattacca tcatcccact gatcagccag accaaccaca agctgatgtc cgatgacaaa
      601 cgccacgaac tgattcaccg tatccagttt ggtggtgacg aagttgtgaa agccaagaac
      661 ggtgcaggca gcgcaactct gtctatggct catgcaggtg ctaagttcgc caacgcagtt
      721 ctgagcggtt tcaaaggtga acgcgatgta atcgagccgt ctttcgtaga ctctccgctg
      781 ttcaaatctg agggcatcga gttcttcgcg tctccggtga ccctgggtcc ggatggtatt
      841 gagaagattc acccgatcgg tgaactgtct agcgaagagg aggagatgct gcaaaaatgc
      901 aaagaaactc tgaagaagaa cattgagaaa ggcgtaaact ttgtagcatc caaggagaac
      961 ctgtacttcc aaggtcatca tcaccaccat cac
//
