LOCUS       sequence-396481-        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of yeast cytosolic malate dehydrogenase; Use in
            CURES (integration of research into undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET28a MDHC_YEAST
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1132..1152
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1153..1170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgccacact ccgttactcc atctattgaa caggattctc tgaagattgc aatcctgggt
       61 gctgctggtg gcatcggtca gagcctgtcc ctgctgctga aggcacagct gcaataccag
      121 ctgaaagaat ctaaccgttc cgttacccac attcacctgg ctctgtatga cgttaaccag
      181 gaagcgatca acggtgtaac cgctgatctg tctcacattg acactccgat ctctgtatcc
      241 agccactctc cagctggtgg tatcgagaac tgtctgcaca acgccagcat tgtagttatc
      301 ccagcaggcg ttccacgtaa gccaggcatg acccgtgacg acctgttcaa cgtgaatgca
      361 ggtatcatca gccagctggg tgatagcatt gcggagtgct gcgacctgtc taaagtgttt
      421 gttctggtga tttctaatcc ggttaactcc ctggttccgg ttatggtaag caacattctg
      481 aagaaccatc cgcagtctcg caactccggt atcgagcgtc gtatcatggg tgtgaccaag
      541 ctggatatcg ttcgtgcgag cactttcctg cgtgagatca acatcgaatc tggtctgact
      601 ccacgtgtga actctatgcc agatgttccg gtgattggtg gtcattctgg tgaaaccatc
      661 attccgctgt tctcccaatc caacttcctg tcccgtctga acgaagacca actgaagtac
      721 ctgattcatc gtgttcagta cggtggcgat gaagtggtta aggcgaagaa cggcaaaggt
      781 tctgcgactc tgtctatggc acacgcaggc tacaaatgtg ttgtgcagtt cgtgagcctg
      841 ctgctgggca acattgaaca aatccacggt acttactatg ttccgctgaa agatgcaaac
      901 aacttcccga ttgctccagg tgcggatcag ctgctgccac tggttgacgg tgcagactac
      961 ttcgccatcc cactgaccat caccactaaa ggcgtgtctt acgttgacta tgacattgta
     1021 aaccgcatga acgacatgga gcgcaaccag atgctgccga tctgtgtttc tcagctgaag
     1081 aagaacatcg acaaaggtct ggagttcgtt gcgtctcgct ccgctagctc tgagaacctg
     1141 tacttccaag gtcatcatca ccaccatcac
//
