LOCUS       sequence-396483-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use
            in CURES (integration of research into undergraduate teaching 
            labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET28a MDHP_YEAST
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1030..1050
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1051..1068
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggtgaaag ttgctattct gggtgcttct ggtggtgttg gtcaaccact gtctctgctg
       61 ctgaaactgt ctccgtacgt ttctgagctg gctctgtacg acattcgtgc tgctgagggt
      121 atcggcaagg atctgagcca catcaacacc aattcctcct gtgtaggtta tgataaggac
      181 agcatcgaga acaccctgtc caacgcacaa gttgtactga ttccagcagg tgtgccacgt
      241 aaaccaggtc tgacccgtga tgatctgttc aagatgaacg ctggcatcgt taagagcctg
      301 gtgaccgctg taggcaagtt cgcaccgaac gctcgtattc tggttatctc caatccggtg
      361 aacagcctgg tgccaatcgc agtggaaacc ctgaagaaga tgggcaagtt caagccaggc
      421 aacgttatgg gtgtaaccaa cctggatctg gtgcgtgcag agactttcct ggtggactac
      481 ctgatgctga agaatccgaa gattggccag gaacaggaca agaccactat gcaccgcaaa
      541 gtgaccgtga ttggtggtca tagcggtgaa accatcatcc cgatcatcac tgacaaatct
      601 ctggtgttcc agctggacaa acaatacgag cacttcatcc accgtgttca gttcggtggc
      661 gatgaaatcg tgaaagcgaa acagggtgct ggttctgcta ctctgagcat ggcgttcgct
      721 ggtgcgaagt tcgctgaaga agtactgcgt tccttccaca atgagaaacc ggaaactgaa
      781 tctctgagcg cattcgttta cctgccaggc ctgaagaacg gtaagaaagc acagcagctg
      841 gttggcgata actctatcga atactttagc ctgccgatcg ttctgcgcaa tggctctgta
      901 gttagcattg acaccagcgt gctggagaag ctgtctccac gcgaagaaca gctggttaac
      961 accgcagtta aggaactgcg taagaacatt gagaaaggca aatctttcat tctggacagc
     1021 tccaaactgg agaacctgta cttccaaggt catcatcacc accatcac
//
