LOCUS       sequence-396487-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of unicellular protozoan parasite P. falciparum
            malate dehydrogenase; Use in CURES (integration of research into 
            undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET28a MDH_PFALCI
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             940..960
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             961..978
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaccaaga tcgctctgat cggttctggc caaatcggtg caattgtagg cgaactgtgt
       61 ctgctggaga acctgggtga cctgatcctg tacgacgtag ttccaggtat tccgcaaggt
      121 aaagcgctgg acctgaagca ctttagcacc atcctgggtg tgaatcgcaa catcctgggt
      181 actaatcaga tcgaagacat caaggatgct gacatcatcg ttatcaccgc tggtgtacag
      241 cgtaaggagg gcatgacccg tgaggacctg attggcgtaa acggcaagat catgaaatct
      301 gtagctgagt ccgtgaaact gcattgttct aaagcgttcg taatctgcgt gtctaaccca
      361 ctggatatca tggtaaacgt attccacaag ttcagcaacc tgccacacga gaagatttgc
      421 ggtatggctg gtatcctgga tacttctcgt tactgttccc tgattgcaga caaactgaaa
      481 gttagcgcag aggatgtgaa cgcagtgatc ctgggtggtc atggcgacct gatggtgcca
      541 ctgcaacgct acacttccgt taacggtgta ccactgagcg agtttgttaa gaagaacatg
      601 atcagccaga acgaaatcca agaaatcatc cagaagactc gtaacatggg tgcggagatc
      661 atcaaactgg cgaaagcctc tgctgctttc gcaccagcag cagctatcac caagatgatc
      721 aagtcctacc tgtacaacga gaacaatctg ttcacctgtg cggtgtacct gaacggtcat
      781 tacaattgca gcaacctgtt cgttggctct actgcgaaga tcaacaacaa aggtgcgcat
      841 ccggttgagt tcccactgac taaagaagaa caggatctgt acactgaatc tatcgcaagc
      901 gtgcagagca acacccagaa ggccttcgac ctgatcaagg agaacctgta cttccaaggt
      961 catcatcacc accatcac
//
