LOCUS       sequence-396489-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Arabidopsis mitochondrial malate 
            dehydrogenase; Use in CURES (integration of research into 
            undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET24a MDHM1_ARATH
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             961..978
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtcctctg gttctgtacc ggaacgtaaa gtagctattc tgggtgctgc tggtggcatc
       61 ggtcaaccac tggctctgct gatgaaactg aacccactgg tatccagcct gtctctgtac
      121 gacatcgcaa acactccagg tgttgcagct gacgttggtc acatcaacac tcgctccgaa
      181 gttgtaggct acatgggtga cgacaacctg gcgaaagcgc tggaaggtgc agacctggta
      241 atcattccag caggtgttcc acgtaaacca ggtatgaccc gtgatgatct gttcaacatc
      301 aacgcaggca tcgtgaagaa tctgtgtact gcgattgcga agtattgtcc acatgcgctg
      361 atcaacatga ttagcaatcc ggtaaacagc accgtaccga tcgcagctga aatcttcaag
      421 aaagctggta tgtatgacga gaagaagctg ttcggtgtta ccaccctgga cgtagtgcgt
      481 gctcgtacct tctacgcagg taaagctaat gttccggttg cggaggtgaa cgttccggtg
      541 attggtggtc atgcaggtgt gaccatcctg ccactgttta gccaggcgac tccgcaggct
      601 aacctgtctt ccgacatcct gactgccctg accaaacgta ctcaggacgg tggcactgaa
      661 gtggtggaag cgaaagctgg taaaggctcc gcaaccctga gcatggctta tgcaggtgct
      721 ctgttcgcag atgcgtgcct gaaaggcctg aacggtgtac cggacgtgat cgaatgctcc
      781 tatgtacagt ctaccatcac tgaactgccg ttcttcgcat ctaaagttcg cctgggcaag
      841 aacggtgtgg aagaagtgct ggacctgggt ccactgagcg acttcgagaa agaaggcctg
      901 gaagcgctga aaccggaact gaaatctagc atcgagaaag gtgtgaagtt cgcaaaccag
      961 catcatcacc accatcac
//
