LOCUS       sequence-396491-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Arabidopsis cytosolic malate dehydrogenase; 
            Use in CURES (integration of research into undergraduate teaching 
            labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET24a MDHC1_ARATH
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             997..1014
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctaaag aaccagttcg tgtactggta actggtgcag caggtcagat cggctatgcg
       61 ctggttccga tgattgcacg tggtatcatg ctgggtgcag accagccagt gatcctgcac
      121 atgctggaca tcccaccagc agcggaagcg ctgaacggtg ttaagatgga actgatcgac
      181 gcagctttcc cactgctgaa aggtgttgtt gctaccaccg acgctgtaga aggttgtact
      241 ggtgtaaacg ttgcggttat ggttggtggc tttccacgta aagagggcat ggaacgtaaa
      301 gatgtaatgt ccaagaacgt tagcatctac aaatctcagg cagcagcact ggagaaacat
      361 gcagcaccaa actgtaaagt gctggtagtg gcgaatccag caaacaccaa cgcactgatt
      421 ctgaaagagt tcgcaccgtc cattccggag aagaacatct cctgcctgac tcgtctggac
      481 cacaaccgtg ccctgggtca gatcagcgaa cgtctgtccg ttccggtgtc tgatgtaaag
      541 aacgttatca tctggggtaa tcactccagc tctcagtatc cggatgtgaa ccacgctaaa
      601 gtgcaaacct cttctggtga gaaaccagtt cgtgaactgg ttaaggacga tgcatggctg
      661 gatggtgagt tcattagcac cgttcagcag cgtggtgctg ccatcatcaa agcacgtaag
      721 ctgtccagcg ctctgtctgc tgcctcctct gcatgtgatc acattcgtga ctgggttctg
      781 ggcactccag agggtacttt cgtttctatg ggtgtttaca gcgacggctc ctactctgta
      841 ccgagcggtc tgatctactc ctttccggtg acttgtcgca atggcgactg gtccatcgtg
      901 caaggtctgc caattgacga agtatctcgc aagaagatgg acctgactgc cgaagaactg
      961 aaagaagaga aagacctggc atacagctgc ctgtctcatc atcaccacca tcactaa
//
