LOCUS       sequence-396493-        1197 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Arabidopsis chloroplastic malate 
            dehydrogenase; Use in CURES (integration of research into 
            undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1197)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET24a MDHNP_ARATH
FEATURES             Location/Qualifiers
     source          1..1197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1177..1194
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtgttccg tttcccagaa cagccaagct ccagttgcag ttcaagagaa cggtctggtt
       61 aagaccaaga aagagtgcta tggcgtgttc tgcctgacct acgacctgaa agcggaagaa
      121 gaaacccgtt cttggaagaa actgatcaac attgccgttt ccggtgctgc tggtatgatt
      181 tctaaccacc tgctgttcaa gctggcttct ggtgaagtgt ttggtccgga ccagccaatc
      241 gcactgaaac tgctgggctc cgaacgttcc attcaagctc tggaaggcgt ggcgatggaa
      301 ctggaagatt ccctgttccc actgctgcgt gaggttgata tcggcactga cccgaacgaa
      361 gtgttccagg acgttgaatg ggcaatcctg atcggtgcga aaccacgtgg tccaggtatg
      421 gaacgtgcag acctgctgga catcaacggt caaatcttcg ctgaacaggg caaagcgctg
      481 aacaaagcag ccagcccaaa cgttaaggtt ctggtggtgg gcaacccgtg caatactaac
      541 gcactgattt gtctgaagaa cgcaccaaac atcccagcca agaacttcca tgcactgacc
      601 cgtctggacg agaaccgtgc gaaatgccaa ctggcactga aagcaggcgt gttctatgac
      661 aaagttagca acatgaccat ttggggtaat catagcacca cccaggtgcc agactttctg
      721 aacgctcgta tcaacggtct gccagtaaag gaagtaatca ccgatcacaa atggctggaa
      781 gaaggcttta ccgaatctgt tcagaaacgt ggtggcctgc tgatccagaa atggggtcgc
      841 tcttctgctg cgagcaccgc agtgagcatt gtagatgcta tcaaatctct ggttactcca
      901 actccggagg gtgactggtt tagcactggt gtttacaccg acggcaaccc gtatggcatc
      961 gaagaaggtc tggtgttctc tatgccgtgc cgtagcaaag gtgacggtga ctacgagctg
     1021 gtgaaagacg ttgaaatcga cgactacctg cgtcaacgta tcgctaagtc cgaagcagaa
     1081 ctgctggcag agaaacgctg tgttgctcac ctgactggtg aaggcatcgc gtattgcgac
     1141 ctgggtccgg tagataccat gctgccaggt gaagtgcatc atcaccacca tcactaa
//
