LOCUS       sequence-396495-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of A. arcticum malate dehydrogenase; Use in 
            CURES (integration of research into undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET24a MDH_AQUAR
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             988..1008
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atggcgaaga ctccgatgcg tgtagcggtt actggtgcag caggtcaaat ctgctactcc
       61 ctgctgtttc gtattgcaaa cggtgacatg ctgggcaaag accagccagt gatcctgcag
      121 ctgctggaga tcccgaacga gaaagcgcag aaagcgctgc aaggcgtgat gatggagatc
      181 gatgattgcg ctttcccact gctggctggt atgactgctc atgccgaccc aatgaccgct
      241 ttcaaggatg ctgatgtggc tctgctggtt ggtgcacgtc cacgtggtcc aggtatggaa
      301 cgtaaagacc tgctggaagc gaacgctcaa atcttcactg ttcaaggtaa agcgattgat
      361 gcggtagcgt cccgtaacat caaggttctg gtagtgggta acccagcgaa caccaacgcc
      421 tacatcgcta tgaaatctgc accgtctctg ccagcgaaga acttcaccgc tatgctgcgt
      481 ctggaccaca atcgtgccct gtcccaaatc gcagcaaaga ctggtaaacc agtgtcctct
      541 atcgagaaac tgttcgtttg gggtaaccat tctccgacca tgtatgccga ttaccgctat
      601 gcacagatcg acggtgcttc tgttaaggat atgatcaacg atgatgcttg gaatcgcgac
      661 accttcctgc cgaccgtagg caaacgtggt gctgctatca tcgatgcacg tggcgtttcc
      721 tccgctgcgt ctgctgcaaa tgcagctatc gaccatatcc acgactgggt tctgggcacc
      781 gctggcaaat ggaccactat gggcatcccg agcgatggca gctacggcat tccggaaggt
      841 gttatcttcg gcttcccagt aaccactgag aacggtgagt acaagatcgt gcagggtctg
      901 tccatcgacg cattctctca ggagcgcatc aacgttaccc tgaacgaact gctggaagaa
      961 cagaacggtg tacagcacct gctgggtgag aacctgtact tccaaggtca tcatcaccac
     1021 catca
//
