LOCUS       sequence-396497-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of S. avermitilis malate dehydrogenase; Use in 
            CURES (integration of research into undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET24a MDH_STRAW
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             988..1008
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1009..1026
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgactcgta ctccggttaa cgttactgtt actggtgctg ctggtcagat cggctacgca
       61 ctgctgttcc gcattgcgag cggtcaactg ctgggtgcgg atgttccggt aaagctgcgt
      121 ctgctggaaa tcactccagc tctgaaagca gcagaaggca ccgctatgga actggatgac
      181 tgtgcgttcc cactgctgca aggcatcgac atcactgacg acccgaacgt tgccttcgac
      241 ggcaccaacg taggtctgct ggttggtgca cgtccacgta ctaaaggcat ggagcgtggc
      301 gacctgctgt ccgctaacgg tggcatcttc aaaccgcagg gcaaagctat caatgacaac
      361 gctgcagatg acgtgaagat tctggtggtg ggcaatccag caaacactaa tgccctgatt
      421 gctcaggcag ctgcaccgga tgttccagcg gaacgtttca ctgcaatgac tcgtctggat
      481 cacaatcgtg ctctgaccca gctggctaag aagactggta gcactgttgc ggacatcaaa
      541 cgcctgacca tttggggtaa ccacagcgca acccagtatc cggacatctt ccacgcatcc
      601 gttgcaggta agaacgcagc agaagttgtt aacgacgaga agtggctggc tgaagacttc
      661 attccaaccg tggctaaacg tggtgctgct atcattgaag ctcgtggtgc ttctagcgca
      721 gcctctgctg ctaacgctgc gattgaccac gtttacacct gggtgaacgg caccgcagac
      781 ggtgactgga cctctatggg tattccgagc gatggctctt acggtgttcc ggaaggcctg
      841 attagctcct ttccggtaac caccaaggat ggtcgttacg aaatcgtaca gggtctggag
      901 atcaacgagt tctcccgtgc tcgtattgac gcgagcgtta aggaactgga agaggaacgc
      961 gaagcggtgc gtgcgctggg tctgatcgag aacctgtact tccaaggtca tcatcaccac
     1021 catcac
//
