LOCUS       sequence-396499-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of H. marismortui (Dead Sea halophilic red 
            archaeon) cytosolic malate dehydrogenase; Use in CURES (integration 
            of research into undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET24a MDH_HALMA
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             913..933
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgaccaagg ttagcgtagt aggtgcagca ggtactgtgg gtgctgcagc tggttacaac
       61 attgccctgc gtgacattgc ggacgaagtt gtattcgttg acatcccaga caaagaagac
      121 gataccgtgg gtcaggctgc agataccaac cacggcattg cgtatgatag caacacccgt
      181 gttcgccagg gtggctacga agataccgca ggttctgacg ttgtagtgat cactgctggc
      241 atcccacgtc agccaggcca gacccgtatc gatctggcag gcgataacgc accgatcatg
      301 gaagacatcc agtcctccct ggacgaacac aacgatgatt acatctccct gactacttcc
      361 aatccggttg atctgctgaa ccgtcatctg tacgaggcag gtgaccgctc ccgtgagcaa
      421 gtaatcggct tcggtggtcg tctggactct gctcgtttcc gttatgttct gtccgaagag
      481 ttcgacgctc cggtgcagaa cgtggagggc accatcctgg gtgaacacgg tgacgctcaa
      541 gttccagtat tctccaaagt tcgcgtggac ggcaccgatc cggagttcag cggtgacgag
      601 aaagaacaac tgctgggtga cctgcaagaa tctgctatgg atgtaatcga acgtaaaggt
      661 gctactgaat ggggcccagc gcgtggtgta gctcacatgg tggaagcgat tctgcacgac
      721 actggcgaag ttctgccagc gtccgttaag ctggagggtg agttcggtca cgaagacact
      781 gccttcggtg ttccggtacg tctgggtagc aacggtgtgg aagaaatcgt tgaatgggac
      841 ctggatgatt acgaacagga cctgatggca gatgctgcgg agaagctgag cgaccagtac
      901 gacaagatca gcgagaacct gtacttccaa ggtcatcatc accaccatca
//
