LOCUS       sequence-396501-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of C. parvum (green sulfur photosynthetic 
            bacterium) malate dehydrogenase; Use in CURES (integration of 
            research into undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET24a MDH_CHLP8
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             931..951
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             952..969
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaagatca ctgttatcgg tgctggtaac gtgggtgcaa ctactgcatt ccgtatcgca
       61 gacaagaaac tggctcgcga actggtgctg ctggacgtgg tggagggtat tccgcagggc
      121 aaaggtctgg atatgtatga aactggtccg gttggcctgt tcgataccaa gatcactggc
      181 agcaacgact acgcagacac tgcagattct gacattgtta tcattactgc tggcctgcca
      241 cgcaaaccag gtatgacccg tgaagacctg ctgatgaaga acgctggtat cgttaaggaa
      301 gtaaccgata acatcatgaa acactccaag aatccgatca tcatcgtagt atctaaccca
      361 ctggacatca tgacccatgt tgcttgggtt cgttccggtc tgccgaaaga acgcgttatc
      421 ggtatggctg gtgtgctgga tgcagcacgt ttccgtagct tcatcgctat ggagctgggt
      481 gttagcatgc aggacatcaa cgcgtgtgtg ctgggtggtc atggtgacgc tatggtgcca
      541 gttgtgaagt ataccactgt tgctggtatc ccgatctccg atctgctgcc agctgaaacc
      601 atcgacaaac tggttgaacg tacccgtaac ggtggtgcag agatcgttga acatctgaag
      661 cagggttctg cgttctacgc tccagcatcc tctgttgttg agatggtaga atctattgtg
      721 ctggatcgta aacgcgtact gccgtgtgcc gttggtctgg aaggtcagta cggtatcgac
      781 aagacctttg tgggtgttcc agtgaaactg ggtcgcaatg gcgtagaaca aatctacgaa
      841 atcaacctgg atcaggctga cctggatctg ctgcagaagt ctgcgaagat cgtggatgag
      901 aactgtaaga tgctggagag caccatcggt gagaacctgt acttccaagg tcatcatcac
      961 caccatcac
//
