LOCUS       sequence-396503-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of I. islandicus malate dehydrogenase; Use in 
            CURES (integration of research into undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET24a MDH_9CREN
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             931..948
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggcacgta ttccgtacaa ggtagctgta atcggtactg gtcgtgttgg tgcaactttc
       61 gcgtacacta tggcagttgt tccaggtatt gctcgtatga ccctggttga tgttgttcca
      121 ggcctggcga aaggcgtaat ggaagatatc aagcacgctg ctgctgtatt ccgtcgctct
      181 atcaccgtag aagcgtttga agacgtatct aaagttgaga atgcagacgc gattgttatc
      241 accgcaggta aaccacgtaa agcagacatg tctcgtcgtg atctggcaaa tgtgaacgca
      301 cagatcatcc gtgacattgg cgataagctg cgtgatcgta acccaggtgc tctgtacgtt
      361 gttgttacta acccagttga cgtaatgact atggtgctgg atgatgttat cggttccaag
      421 ggtactgtta tcggcactgg tactagcctg gacacctttc gctttcgtgc tgcggtatct
      481 gaactgctga atgttccgat cgttgccgtg gacggctatg tggttggtga acacggtgaa
      541 gaggctttcg tggcgtggag cactgttact atcaaaggta tccacattga ccagtacatc
      601 aaggagcgta acatcaacat cagccgtgaa cagatcgaga agtacgtgaa ggacgttgca
      661 gcttccatca tcgcttctca gggtgccacc atttggggtc cggcagcaac tttccaggaa
      721 atcgttgtgt ctcacctggc aaacgagtcc aagatcatcc cgatctccct gccacagaac
      781 attgaaggcg ttggtcgtgt ggctgtttct gttccgacca tcatctctgg tcgtctgaaa
      841 ccgctggtgc aactgctgaa cgaagaagaa caggagcgtc tgaagcgtgc agcgaaagcc
      901 atccgcaatg tttacgaaag cattctgacc catcatcacc accatcac
//
