LOCUS       sequence-396505-        1707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a fusion of watermelon glyoxysomal malate 
            dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration 
            of research into undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1707)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pQE60 MGH
FEATURES             Location/Qualifiers
     source          1..1707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             452..463
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(1000..1028)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1623..1643
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1690..1707
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctaaag gcggagctcc cgggttcaaa gtcgcaatac ttggcgctgc cggtggcatt
       61 ggccagcccc ttgcgatgtt gatgaagatg aatcctctgg tttctgttct acatctatat
      121 gatgtagtca atgcccctgg tgtcaccgct gatattagcc acatggacac gggtgctgtg
      181 gtgcgtggat tcttggggca gcagcagctg gaggctgcgc ttactggcat ggatcttatt
      241 atagtccctg caggtgttcc tcgaaaacca ggaatgacga gggatgatct gttcaaaata
      301 aacgcaggaa ttgtcaagac tctgtgtgaa gggattgcaa agtgttgtcc aagagccatt
      361 gtcaacctga tcagtaatcc tgtgaactcc accgtgccca tcgcagctga agttttcaag
      421 aaggctggaa cttatgatcc aaagcgactt ctgggagtta caatgctcga cgtagtcaga
      481 gccaatacct ttgtggcaga agtattgggt cttgatcctc gggatgttga tgttccagtt
      541 gttggcggtc atgctggtgt aaccattttg ccccttctat ctcaggtgaa gcctccaagt
      601 tctttcacac aagaagagat tagttacctg actgatagga ttcaaaatgg tggaacagaa
      661 gttgtcgagg ccaaagcagg agctggttca gcaactctct caatggctta tgctgccgtt
      721 aagtttgcag atgcatgcct caggggctta agaggagatg ctggtgtcat tgaatgcgcg
      781 tttgtgtctt ctcaggtgac tgaacttcca ttctttgcat caaaagtacg acttggtcgc
      841 aatggtatcg aagaagtata ctcccttggc ccgctaaatg agtatgagag gattggattg
      901 gagaaagcga agaaagagtt ggcaggaagc attgagaagg gagtttcctt catcagaagc
      961 agatctatgg ctagcaaagg agaagaactc ttcactggag ttgtcccaat tcttgttgaa
     1021 ttagatggtg atgttaacgg ccacaagttc tctgtcagtg gagagggtga aggtgatgca
     1081 acatacggaa aacttaccct gaagttcatc tgcactactg gcaaactgcc tgttccatgg
     1141 ccaacactag tcactactct gtgctatggt gttcaatgct tttcaagata cccggatcat
     1201 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaaggacc
     1261 atcttcttca aagatgacgg caactacaag acacgtgctg aagtcaagtt tgaaggtgat
     1321 acccttgtta atagaatcga gttaaaaggt attgacttca aggaagatgg caacattctg
     1381 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     1441 aagaatggaa tcaaagtgaa cttcaagacc cgccacaaca ttgaagatgg aagcgttcaa
     1501 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1561 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1621 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactgtac
     1681 aacggatctc atcaccatca ccatcac
//
