LOCUS       sequence-396541-        9691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1
            Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive 
            selection and HSV-TK for negative selection. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Wan Y, Cohen J, Szenk M, Farquhar KS, Coraci D, Krzyszton R, Azukas 
            J, Van Nest N, Smashnov A, Chern YJ, De Martino D, Nguyen LC, Bien 
            H, Bravo-Cordero JJ, Chan CH, Rosner MR, Balazsi G
  TITLE     Nonmonotone invasion landscape by noise-aware control of metastasis 
            activator levels.
  JOURNAL   Nat Chem Biol. 2023 Jul;19(7):887-899. doi: 
            10.1038/s41589-023-01344-z. Epub 2023 May 25.
  PUBMED    37231268
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01344-z"; journalName: "Nat Chem Biol"; date: 
            "2023-07"; volume: "19"; issue: "7"; pages: "887-899"
COMMENT     Sequence Label: pUt-mPF-BACH1 Cas9-Resistant_AmpR
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(11..13,14..16,17..727)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11..13,14..16,17..727)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(56..77)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(317..336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(3030..3050)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(3403..3420)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3409..3633
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3768..4162
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      4598..4732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4602..4899)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     4897..4917
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     protein_bind    complement(4945..4978)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        5045..5149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5150..5218,5219..6010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5368..5387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        6043..6542
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6078..6101)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6475..6494
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     5'UTR           6584..6623
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             6641..7771
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(7769..7788)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7813..7861
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7885..8473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8374..8393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(8480..8513)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        8573..8887
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    8581..8599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8617..8635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8652..8670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8688..8706
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8724..8742
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8759..8777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8795..8813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8860..8884
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(8892..9509,9510..9515,9516..9551,9552..9554,9555..9590,
                     9591..9593,9594..9629,9630..9635)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             join(9645..9691,1..10)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ccctggacct atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
       61 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      121 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      181 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
      241 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      301 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      361 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      421 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
      481 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
      541 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      601 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
      661 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
      721 gtacaagggt gacggtgctg gtttaattaa catgtctctg agtgagaact cggtttttgc
      781 ctatgaatct tctgtgcata gcaccaatgt tttactcagc cttaatgacc agcggaagaa
      841 agatgtgctg tgcgatgtca ccatctttgt ggagggacag cggttccgcg ctcaccggtc
      901 cgtgctggcg gcatgcagca gttactttca ctcaagaatc gtaggccagg ctgatggaga
      961 gctgaacatt actcttccag aagaggtgac agttaaagga tttgaacctt taattcagtt
     1021 tgcctacact gctaaactga ttttaagtaa agagaatgtg gatgaagtgt gcaaatgtgt
     1081 ggagttttta agtgtacata atattgagga atcctgcttt cagtttctga aatttaagtt
     1141 tttggactcc actgcagacc agcaagaatg cccaagaaaa aaatgctttt catcacactg
     1201 tcagaaaaca gaccttaaac tttcactttt ggaccagagg gatctagaaa ctgatgaagt
     1261 ggaggaattt ctggaaaata aaaatgttca gactcctcag tgtaaactcc gcaggtatca
     1321 aggaaatgca aaagcctcac ctcctctaca agacagtgcc agtcagacat atgagtccat
     1381 gtgcttagag aaggatgctg ctctggcctt gccttcttta tgccccaaat acagaaaatt
     1441 ccaaaaagca tttggaactg acagagtccg tactggggaa tctagtgtca aagacattca
     1501 tgcttctgtt cagccaaatg aaaggtctga aaatgaatgc ctgggaggag tcccggagtg
     1561 tagagatttg caggtgatgt taaaatgtga cgaaagtaaa ttagcaatgg aacctgaaga
     1621 aacgaagaaa gatcctgctt ctcagtgccc aactgaaaaa tcagaagtga ctcctttccc
     1681 ccacaattct tccatagacc ctcatggact ttattctttg tctcttttac acacatatga
     1741 ccaatatggt gacttgaatt ttgctggtat gcaaaacaca acagtgttaa cagaaaagcc
     1801 tttgtcaggt acagacgtcc aagaaaaaac atttggtgaa agtcaggatt tacctttgaa
     1861 atccgacttg ggcaccaggg aagatagtag tgttgcatct agtgatagga gtagtgtgga
     1921 gcgagaagtg gcagaacacc tagcaaaagg cttctggagt gacatttgca gcacggacac
     1981 tccttgccaa atgcagttat cacctgctgt ggccaaagat ggctcagaac agatctcaca
     2041 gaaacggtct gagtgtccgt ggttaggtat caggattagt gagagcccag aaccaggtca
     2101 aaggactttc acaacattaa gttctgtcaa ctgccctttt ataagtactc tgagtactga
     2161 aggctgttca agcaatttgg aaattggaaa cgatgattat gtttcagaac cccagcaaga
     2221 accttgccca tatgcttgtg tcattagctt gggagacgac tctgagacgg acaccgaagg
     2281 agacagtgaa tcctgttcag ccagagaaca agaatgtgag gtaaaactgc cattcaatgc
     2341 acaacggata atttcactgt ctcgaaatga ttttcagtcc ttgttgaaaa tgcacaagct
     2401 tactccagaa cagctggatt gtatccatga tattcgaaga agaagtaaaa acagaattgc
     2461 tgcacagcgc tgtcgcaaga gaaaacttga ctgtatacag aatcttgaat cagaaattga
     2521 gaagctgcaa agtgaaaagg agagcttgtt gaaggaaaga gatcacattt tgtcaactct
     2581 gggtgagaca aagcagaacc taactggact ttgccagaaa gtttgtaaag aagcagctct
     2641 gagtcaagaa caaatacaga tactcgccaa gtactcagct gcagattgcc cactttcatt
     2701 tttaatttct gaaaaagata aaagtactcc tgatggtgaa ctggcgttac catcaatttt
     2761 cagtttatct gaccggcctc cagcagtgct gcctccctgt gccagaggaa acagtgagcc
     2821 tggctacgcg cgagggcagg agtcccagca gatgtccaca gccacctctg agcaagctgg
     2881 gcctgcggaa cagtgtcgtc agagtggtgg gatctcagat ttctgtcagc agatgactga
     2941 taaatgtact actgatgagt aaaccggtgc ggccgcaatc aacctctgga ttacaaaatt
     3001 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3061 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3121 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3181 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3241 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3361 ttgtcctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     3421 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3481 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3541 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3601 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     3661 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3721 ttacgcgcta aagcaagtaa aacctctaca aatgtggtat ggaattctaa aatacagcat
     3781 agcaaaactt taacctccaa atcaagcctc tacttgaatc cttttctgag ggatgaataa
     3841 ggcataggca tcaggggctg ttgccaatgt gcattagctg tttgcagcct caccttcttt
     3901 catggagttt aagatatagt gtattttccc aaggtttgaa ctagctcttc atttctttat
     3961 gttttaaatg cactgacctc ccacattccc tttttagtaa aatattcaga aataatttaa
     4021 atacatcatt gcaatgaaaa taaatgtttt ttattaggca gaatccagat gctcaaggcc
     4081 cttcataata tcccccagtt tagtagttgg acttagggaa caaaggaacc tttaatagaa
     4141 attggacagc aagaaagcga gcttctagct ttagttcctg gtgtacttga gggggatgag
     4201 ttcctcaatg gtggttttga ccagcttgcc attcatctca atgagcacaa agcagtcagg
     4261 agcatagtca gagatgagct ctctgcacat gccacagggg ctgaccaccc tgatggatct
     4321 gtccacctca tcagagtagg ggtgcctgac agccacaatg gtgtcaaagt ccttctgccc
     4381 gttgctcaca gcagacccaa tggcaatggc ttcagcacag acagtgaccc tgccaatgta
     4441 ggcctcaatg tggacagcag agatgatctc cccagtcttg gtcctgatgg ccgccccgac
     4501 atggtgcttg ttgtcctcat agagcatggt gatcttctca gtggcgacct ccaccagctc
     4561 cagatcctgc tgagagatgt tgaaggtctt catggtagcc tccaaaaaag cctcctcact
     4621 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     4681 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     4741 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     4801 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     4861 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     4921 gctgcgaaga gaagaggtca ggccgaagtt cctatactat ttgaagaata ggaacttcaa
     4981 gcttgcccca ctgtggggtg gaggggacag ataaaagtac cctggcactt ttcggggaaa
     5041 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5101 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5161 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5221 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5281 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5341 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5401 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5461 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5521 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5581 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5641 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ccgtagcaat
     5701 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5761 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5821 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5881 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5941 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6001 gcattggtaa ctgtcagacc aagtttattc tacggttcta ccgggtaggg gaggcgcttt
     6061 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     6121 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     6181 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     6241 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     6301 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     6361 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     6421 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     6481 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     6541 cgacctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatcttg gtggcgtgaa
     6601 actcccgcac ctcttcggca agcgccttgt agaagcgcgt atggcttcgt acccctgcca
     6661 tcaacacgcg tctgcgttcg accaggctgc gcgttctcgc ggccatagca accgacgtac
     6721 ggcgttgcgc cctcgccggc agcaagaagc cacggaagtc cgcctggagc agaaaatgcc
     6781 cacgctactg cgggtttata tagacggtcc tcacgggatg gggaaaacca ccaccacgca
     6841 actgctggtg gccctgggtt cgcgcgacga tatcgtctac gtacccgagc cgatgactta
     6901 ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc tacaccacac aacaccgcct
     6961 cgaccagggt gagatatcgg ccggggacgc ggcggtggta atgacaagcg cccagataac
     7021 aatgggcatg ccttatgccg tgaccgacgc cgttctggct cctcatatcg ggggggaggc
     7081 tgggagctca catgccccgc ccccggccct caccctcatc ttcgaccgcc atcccatcgc
     7141 cgccctcctg tgctacccgg ccgcgcgata ccttatgggc agcatgaccc cccaggccgt
     7201 gctggcgttc gtggccctca tcccgccgac cttgcccggc acaaacatcg tgttgggggc
     7261 ccttccggag gacagacaca tcgaccgcct ggccaaacgc cagcgccccg gcgagcggct
     7321 tgacctggct atgctggccg cgattcgccg cgtttacggg ctgcttgcca atacggtgcg
     7381 gtatctgcag ggcggcgggt cgtggcggga ggattgggga cagctttctg ggacggccgt
     7441 gccgccccag ggtgccgagc cccagagcaa cgcgggccca cgaccccata tcggggacac
     7501 gttatttacc ctgtttcggg cccccgagtt gctggccccc aacggcgacc tgtacaacgt
     7561 gtttgcctgg gccttggacg tcttggccaa acgcctccgt cccatgcacg tctttatcct
     7621 ggattacgac caatcgcccg ccggctgccg ggacgccctg ctgcaactta cctccgggat
     7681 ggtccagacc cacgtcacca cccccggctc cataccgacg atctgcgacc tggcgcgcac
     7741 gtttgcccgg gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     7801 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
     7861 cataaacgcg ctcataggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7921 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7981 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8041 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8101 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8161 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8221 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8281 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8341 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8401 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8461 gagcctatgg aaaaacgccg aagttcctat actttctaga gaataggaac ttcacgggcc
     8521 agatatacgc gacgggccag atatacgcgt cgaggccctt tcgtcttcac tcgagtttac
     8581 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
     8641 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     8701 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
     8761 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     8821 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     8881 gatcgcctgg aatgtctaga ctggacaaga gcaaagtcat aaacggcgct ctggaattac
     8941 tcaatggagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     9001 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
     9061 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     9121 actttctgcg gaacaacgcc aagtcattcc gctgtgctct cctctcacat cgcgacgggg
     9181 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     9241 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     9301 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
     9361 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     9421 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     9481 tggagaaaca gctaaagtgc gaaagcggcg ggccggccga cgcccttgac gattttgact
     9541 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     9601 ctcttgacga ttttgacctt gacatgctcc ccgggggaag cggagctact aacttcagcc
     9661 tgctgaagca ggctggagac gtggaggaga a
//
