LOCUS       sequence-396548-       11144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for lncRNA NORAD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Tichon A, Gil N, Lubelsky Y, Havkin Solomon T, Lemze D, Itzkovitz S,
            Stern-Ginossar N, Ulitsky I
  TITLE     A conserved abundant cytoplasmic long noncoding RNA modulates 
            repression by Pumilio proteins in human cells.
  JOURNAL   Nat Commun. 2016 Jul 13;7:12209. doi: 10.1038/ncomms12209.
  PUBMED    27406171
REFERENCE   2  (bases 1 to 11144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-13"; volume: "7:12209. doi"; pages: " 
            10.1038/ncomms12209"
COMMENT     Sequence Label: pcDNA3.1-NORAD
FEATURES             Location/Qualifiers
     source          1..11144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2962..2973
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6501..6518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6507..6731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6777..7205
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6864..6883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7074..7095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7214..7234)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7219..7548
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7399..7534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7615..8409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7669..7688)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8279..8298
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8583..8704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8620..8639)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8674..8693
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8753..8769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8753..8769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8766..8788)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8777..8793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8801..8807,8808..8825,8826..8831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8846..8867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8984..9001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9155..9743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9235..9254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9914..10705,10706..10774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10537..10556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10775..10879)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10954..10973)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gatctgcaga attcgccctt tgccagcgca gagaactgcc aagtcagttc cggtccggca
      721 gagatcgcgg agagacgcag aacgcagccc gctcctccag ggccctccag gccctccggc
      781 cccgggccgg cgggtgaact ggggggcccc gggacaggcc gagccctctg ccctgcagat
      841 aacggaggcc tctgctgtgg ctgcccactg gctgtgcccg cccactggct gtgcccagac
      901 cttgaagccg cagcgaacct ctctttccca ccccacctcg gtgactaatg gcggccgtgg
      961 cgtctcccag cccggacccc gccggcaccc gggtctcccg acccaagcct cgacgaaacc
     1021 cccgcagagc cgccgggacg cagcgccttt gggcggcgct gggcgtggtg ggccgggaag
     1081 tatggcggca gctcgaacgc cgcgcggcgg aggccattaa ggcgtggacg gcccgggaag
     1141 gcggcctagg gacgcaagca ggctcggccg cctctttagg ccacggagcc gcgcagatcc
     1201 ggttcccggg tgaccactct gtcgccattg ggcgagacct acctagtcct gacgacaacg
     1261 gacaaaggcc ttaaggggcc tggaaggtga gcgaagtccc gaacgacgac gggtggaacg
     1321 gttagcggcc atcgggcggt tggtcttcat tctaccagac tttgctgtcg gaagagagaa
     1381 atggtagaat gacaggccac gtttggcccg ttggaaatgc ccaccaccct ctgggaagat
     1441 ttactggccg tttatggaag gcctgtgtat ataatatgaa aaagctgctc tcaactccac
     1501 cccaaccttt taatagaaaa catttgtcac atctagccct tctagatgga aagaggttgc
     1561 cgacgtatga taaaatagag ttagaaagtt acacatcttg taaattctca tttgtttaaa
     1621 agaaatcata gaaaatacat gtcttctgga gatgactttt ggaaatggag ttgttaagac
     1681 ggcctctgga agcgatacgt ccacgtttgt taagtgggtt agatgacatg gagctggaag
     1741 acctgagaag gaagagaaga aggttctatg ctagactggt catatttaga agacattttc
     1801 atattctatc cattgttttg tgtgcatttt attcctcact actgtgtata tagttgacaa
     1861 tgctaagctt ttttgaaatg tctcttcttt ttagatgttc tgaagtgcct gatatgttaa
     1921 aattagaggt agcaaaatca cattttgtaa ataccttttt gttacaattc ataggaaata
     1981 tttttggggg ggaatggcca aatcacctgt tgagtaatac tcattgtgtt tgtgcagtgg
     2041 ttcaggggag gagagaggag ggggaggtgc agagagctct atgccatcct gtttacagcg
     2101 aggcaagatg aatcattatg tctgtgcatt ttgttttact tatctgtgta tatagtgtac
     2161 ataaaggaca gacgagtcct aattgacaac atctagtctt tctggatgtt aaagaggttg
     2221 ccagtgtatg acaaaagtag agttagtaaa ctaatatatt ttgtacattt tgttttacaa
     2281 gtcctaggaa agattgtctt ctgaaaattt gatgtcttct gggttgatgg agatgggaag
     2341 ggttctaggc cagaatgttc acatttggaa gactctttca aattataact gttgttacat
     2401 gtttgcagtt tattcaagac tgctgtatac atagtagaca aattaactcc ttacttgaaa
     2461 catctagtct atctagatgt ttagaagtgc ccgatgtatg ttaaatgtat aggtagtaaa
     2521 ataccacttt gtaaatatct ttttgctaaa attcatagga aatgcttttg gaaattgaat
     2581 tgtgaagcca cctttgtgaa cagtatagta atgtctatac ttgttcaata gtttagagga
     2641 ggtaggaggg aagaaattgc aaaaggtaat attactagtg tgttcatact tggacatttt
     2701 cagacaccat ttttctatat gttttgtgca ttttgttttg ctctgtatat agtatatata
     2761 atggacaaat agtcctaatt tttcaacatc tagtctctag atgttaaaga ggttgccagt
     2821 gtatgacaaa ggagtaaaat tagcatattt tgtacacttt gtgttgaaat tcgtaggaaa
     2881 acttgtcttc tgtaaagact tttgcatagg aatttgtttg accatctcta agcattacac
     2941 gtgcctgtac ttgtccactg gattgaaggc agagaaggaa gggaggaggg aatgattcaa
     3001 ggccaaaatg gccacattta gaagatacct cagatgataa ccattgttat gtgtgtgcaa
     3061 ttttatttaa cagtgctgtg tatgtggtgg aaacgttata tgaaatatct agtctttcta
     3121 gatatttgga agtgcttgat gtatttaaaa gtggtagtag aataacactt tgtaaatagc
     3181 ttttaaaaac tgatgggaaa tgctgtttgg aagtggaatt gttgaaccac ctgggaggtg
     3241 ggagggaaga aattgcaaat ggtgttttgc cattgtttat tagaaaattt cagcttaatc
     3301 cattgtgtat atgttacatg catttcattt aactttgcta tactgtatat attgtatata
     3361 taacggacaa attagtcccg attttataat atctagtctc tagatattaa agaggttgcc
     3421 aatgtatgac agaagtagag ttagtaaact aacacatttt gtacactttg ttaaaatttg
     3481 tagaaaggct gtcttctgaa aaggactttt ggaagtgaga taacatcagc tctaagtgac
     3541 acgtgcctat atccatcagg ttggtggtgg agaggagttg gaaggaatga agggttctag
     3601 accagaatgt tcgtatttag aagacactat cagatataac cattgttaca tgtgtgtagt
     3661 ttattcaacc ctactgtgta tatagcggac aaacttaagt ccttatttga aacatctagt
     3721 ctttctagat gtttagaagt gcacaaagta tgttaaaagt agaggtagta aataacacat
     3781 tttgtagcta tccttttgat atgaaatatt gtcttggaaa ttgatcaatt ctctgagcag
     3841 tacccatttt gatatttgtg ctggttcagg gggaaggagg agcacaaagt gcaaagggct
     3901 ttctaccagt gtccagtgtg tttatgagga ggcacattga ccattgtccc ttatgtctgc
     3961 attttcattt actgtgctgt gtatatagtg tatataagcg gacataggag tcctaattta
     4021 cgtctagtcg atgttaaaaa ggttgccagt atatgacaaa agtagaatta gtaaactact
     4081 acattgagta cactttgtgt taaaattcat agggaagact tcttaaaaac aagtgaaatt
     4141 gttaaaaccc cccctaagca ttacagatgg cttatagctg tccacggggt tggtagaggt
     4201 gggaaaggga agggttctag gccagaatgt tcctatttag aagacactca aattacagtc
     4261 tgtgttatgt atgtatacca tttattcaat gctactgtgt atataatgga aaacttaagt
     4321 ccagtttgaa acatctagtc tttctaggtg tttaaaagtg tacaacgttc tgtcgcagtg
     4381 gcgcatgcct gtaatcccag cactttggga ggccgaggca ggcggatcac gaggtcaaga
     4441 gatcaggacc atcttggcca acatggtgaa accccatctt tactaaaaat acaaaaatta
     4501 gctggtcgtg gtggtgccca cctgtagccc cagttactcg agaggctgag gcaggagaat
     4561 cgcttgaact tgggaggcgg aagttgcagt gagccaagat cgcaccactg cactccagcc
     4621 tggcgacaga gcgaggctcc gtttcaaaaa aaaagtgcac aatgtaggtt aacagtagag
     4681 ggcttaagta acacccctct aagcatttgt tttcagtact tcctaggagt ggttgcattt
     4741 gggaatggaa ttgttaaaac ttgatgctta ggagcgaatg cagactattc attgggtgtt
     4801 tggggtgggg gaaggggggg tgggcagagg aggtatgcag ggagaggggt tctgtgctcc
     4861 tgagattagt tcagatggtc taaccattgt tctatatgtg cattttagtt aatattgtgt
     4921 attaaaggat aagtcttaat gctcaaagta tgttaaaaat agatgtagta aatcagtccc
     4981 tttgtgaatg tccttttgtt agtttttagg aaggcctgtc ctctgggagt gacctttatt
     5041 agtccacccc ttggagctag acatcctgta cttagtcacg gggatggtgg aagagggaga
     5101 agaggaaggg tgaagggaag ggctctttgc tagtatctcc atatctagac gatggtttta
     5161 gatgataacc acaggtctac aagagcgttt ttagtaaagt gcctgtgttc attgtggaca
     5221 aagttattat tttgcaacat ctaagcttta cgaatggggt gacaacttat gataaaaact
     5281 agagctagtg aattagccta tttgtaaata cctttgttat aattgatagg atacatcttg
     5341 gacatggaat tgttaagcca cctctgagca gtgtatgtca ggacttgttc attaggttgg
     5401 cagcagaggg gcagaaggaa ttatacaggt agagatgtat gcagatgtgt ccatatatgt
     5461 ccatattaac gttttgatag ccattgatgt atgcatctct tggctgtact ataagaacac
     5521 attaattcaa tggaaataca ctttgctaat attttaatgg tatagatctg ctaatgaatt
     5581 ctcttaaaaa catactgtat tctgttgctg tgtgtttcat tttaaattga gcattaaggg
     5641 aatgcagcat ttaaatcaga actctgccaa tgcttttatc tagaggcgtg ttgccatttt
     5701 tgtcttatat gaaatttctg tcccaagaaa ggcaggatta catctttttt tttttttttt
     5761 agcagtttga gttggtgtag tgtattcttg gttatcagaa tactcatata gctttgggat
     5821 tttgaattgg taaatattca tgatgtgtga aaaatcatga tacatactgt acagtctcag
     5881 tcccataaaa ttggatgttg tgcctacaca caggatctag aagaatatgt caaactataa
     5941 actgcttgtg attgtgaatg actttgttct ttgcttgtgt ttttcaattt cctataatgc
     6001 acatactaac ttttaaaaaa taaaggttat tttaaaagcc tgtattaagc cctcgttgct
     6061 tgtagaatag agttagtata cgctacagaa gcacaggttc atgctccttc atgcttgggc
     6121 tctgaggtat ctggaatttc tcatctttga ctcctccctc cattgcctgg gtgttagcca
     6181 ctgccaccca ggtgctctgc tgttcaggat aaccttgcac tttacaggtt ctgtggtttg
     6241 ctcaagccat tccttctgcc tgtaatgccc tttccacagt ttcacttggc aaaatcattc
     6301 ttcagagccc aacttaaatt cagtatctcc acaaagtctt gcctgtaaga acctgacact
     6361 cccgagtgcc aagggcgaat tccagcacac tggcggccgt tactagtgga tccactagtc
     6421 cagtgtggtg gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     6481 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6541 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6601 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6661 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6721 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     6781 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6841 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6901 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6961 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7021 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7081 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7141 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7201 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7261 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7321 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7381 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7441 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     7501 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     7561 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     7621 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7681 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7741 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7801 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7861 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7921 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7981 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8041 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8101 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8161 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8221 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8281 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8341 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8401 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     8461 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     8521 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     8581 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8701 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8761 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8821 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8881 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    10921 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    10981 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    11041 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
    11101 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
