LOCUS       sequence-396551-       11029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified Tobacco rattle virus (TRV) RNA2 vector for editing of 
            Nicotiana benthamiana Phytoene desaturase (PDS) gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 11029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: SPDK3860
FEATURES             Location/Qualifiers
     source          1..11029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..37
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        566..911
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     854..874
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2484..2559
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3098..3115
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3150..3402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3424..3448
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4749..5378
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5807..6880
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6946..7140
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7376..7398)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7484..7624
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7539..7558
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7639..7656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7810..8398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7890..7909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9823..10617)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
       61 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc ctaggccacc
      121 atgttgggcc cggcgcgcca agcttgcatg cctgcaggtc aacatggtgg agcacgacac
      181 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac
      241 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
      301 cttcatcgaa aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa
      361 aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc
      421 cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
      481 atgtgatggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
      541 cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
      601 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
      661 atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctcta
      721 ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa aaagaagacg
      781 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      841 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      901 tggagaggat aaaacattgc acctatggtg ttgccctggc tggggtatgt cagtgatcgc
      961 agtagaatgt actaattgac aagttggaga atacggtaga acgtccttat ccaacacagc
     1021 ctttatccct ctccctgacg aggtttttgt cagtgtaata tttctttttg aactatccag
     1081 cttagtaccg tacgggaaag tgactggtgt gcttatcttt gaaatgttac tttgggtttc
     1141 ggttctttag gttagtaaga aagcacttgt cttctcatac aaaggaaaac ctgagacgta
     1201 tcgcttacga aagtagcaat gaaagaaagg tggtggtttt aatcgctacc gcaaaaacga
     1261 tggggtcgtt ttaattaact tctcctacgc aagcgtctaa acggacgttg gggttttgct
     1321 agtttcttta gagaaaacta gctaagtctt taatgttatc attagagatg gcataaatat
     1381 aatacttgtg tctgctgata agatcatttt aatttggacg attagacttg ttgaactaca
     1441 ggttactgaa tcacttgcgc taatcaacat gggagatatg tacgatgaat catttgacaa
     1501 gtcgggcggt cctgctgact tgatggacga ttcttgggtg gaatcagttt cgtggaaaga
     1561 tctgttgaag aagttacaca gcataaaatt tgcactacag tctggtagag atgagatcac
     1621 tgggttacta gcggcactga atagacagtg tccttattca ccatatgagc agtttccaga
     1681 taagaaggtg tatttccttt tagactcacg ggctaacagt gctcttggtg tgattcagaa
     1741 cgcttcagcg ttcaagagac gagctgatga gaagaatgca gtggcgggtg ttacaaatat
     1801 tcctgcgaat ccaaacacaa cggttacgac gaaccaaggg agtactacta ctaccaaggc
     1861 gaacactggc tcgactttgg aagaagactt gtacacttat tacaaattcg atgatgcctc
     1921 tacagctttc cacaaatctc taacttcgtt agagaacatg gagttgaaga gttattaccg
     1981 aaggaacttt gagaaagtat tcgggattaa gtttggtgga gcagctgcta gttcatctgc
     2041 accgcctcca gcgagtggag gtccgatacg tcctaatccc tagggattta aggacgtgaa
     2101 ctctgttgag atctctgtga aattcagagg gtgggtgata ccatattcac tgatgccatt
     2161 agcgacatct aaatagggct aattgtgact aatttgaggg aatttccttt accattgacg
     2221 tcgcacacaa ggttaaaaac gctgtagtaa tacatgcgca agaacaggct gagcatcttg
     2281 ttctggggtt tcacactatc tttagagaaa gtgttaagtt aattaagtta tcttaattaa
     2341 gagcataatt atactgattt gtctctcgtt gatagagtct atcattctgt tattaaaaat
     2401 ttgacaactc ggtttgctga cctactggtc actgtatcac ttacccgagt taacgagtct
     2461 agattggtag tagcgactcc atggttttag agctagaaat agcaagttaa aataaggcta
     2521 gtccgttatc aacttgaaaa agtggcaccg agtcggtgcg agctcccgta gctcagttgg
     2581 ttagagcgtt ggtcttatga gccgaaggtc gcgggttcga gccccgccgg aagcacccgg
     2641 gcatgtcccg aagacattaa actacggttc tttaagtaga tccgtgtctg aagttttagg
     2701 ttcaatttaa acctacgaga ttgacattct cgactgatct tgattgatcg gtaagtcttt
     2761 tgtaatttaa ttttcttttt gattttattt taaattgtta tctgtttctg tgtatagact
     2821 gtttgagatc ggcgtttggc cgactcattg tcttaccata ggggaacgga ctttgtttgt
     2881 gttgttattt tatttgtatt ttattaaaat tctcaacgat ctgaaaaagc ctcgcggcta
     2941 agagattgtt ggggggtgag taagtacttt taaagtgatg atggttacaa aggcaaaagg
     3001 ggtaaaaccc ctcgcctacg taagcgttat tacgcccgtc tgtacttata tcagtacact
     3061 gacgagtccc taaaggacga aacgggagaa cgctagccac caccaccacc accacgtgtg
     3121 aattacaggt gaccagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
     3181 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3241 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3301 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3361 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta aactatcagt
     3421 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     3481 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     3541 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     3601 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     3661 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3721 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3781 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3841 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3901 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     3961 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     4021 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     4081 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     4141 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     4201 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     4261 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     4321 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     4381 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     4441 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     4501 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     4561 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     4621 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     4681 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     4741 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     4801 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     4861 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     4921 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     4981 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     5041 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     5101 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     5161 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     5221 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     5281 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     5341 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     5401 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     5461 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     5521 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     5581 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     5641 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     5701 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     5761 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     5821 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     5881 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     5941 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     6001 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     6061 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     6121 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     6181 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     6241 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     6301 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     6361 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     6421 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     6481 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     6541 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     6601 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     6661 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     6721 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     6781 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     6841 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     6901 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
     6961 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     7021 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     7081 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     7141 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     7201 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     7261 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     7321 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     7381 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     7441 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     7501 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     7561 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     7621 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7681 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7741 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7801 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7861 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7921 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7981 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8041 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8101 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8161 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8221 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8281 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8341 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8401 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8461 gctgatgaat cccctaatga tttttatcaa aatcattaag ttaaggtaga tacacatctt
     8521 gtcatatgat caaatggttt cgccaaaaat caataatcag acaacaaaat gtgcgaactc
     8581 gatattttac acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac
     8641 agcttaacgt tggcttgcca cgccttactt gactgtaaaa ctctcactct taccgaactt
     8701 ggccgtaacc tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg
     8761 ttaggtaatc gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt
     8821 atctgttcgg gcaatacgat gcccattgta cttgttgact ggtctgatat ccgtgagcaa
     8881 aaacggctta tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat
     8941 gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc
     9001 gaccttgcga gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt
     9061 aaagtgccat ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga
     9121 ggaaaagtac aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat
     9181 gatatgtcat ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca
     9241 atctcatgcc aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg
     9301 acacggactc attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca
     9361 tggattctag caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc
     9421 tattcgaagc gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga
     9481 ctaggcctac gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc
     9541 gccctgatgc ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg
     9601 gacaagcact tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta
     9661 ggcatggaag ttttgcggca ttctggctac acaataacaa gggaagactc actcgtggct
     9721 gcaaccctgc ttactcaaaa tctattcaca catggttacg ttttggggaa attatgaggg
     9781 gatctctcag cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
     9841 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     9901 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     9961 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    10021 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    10081 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    10141 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    10201 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    10261 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    10321 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    10381 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    10441 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    10501 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    10561 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    10621 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    10681 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    10741 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    10801 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    10861 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    10921 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    10981 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctg
//
