LOCUS       sequence-396552-       11056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with 
            AtPDS3 target for editing Arabidopsis PDS3 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Nagalakshmi U, Meier N, Liu JY, Voytas DF, Dinesh-Kumar SP
  TITLE     High-efficiency multiplex biallelic heritable editing in Arabidopsis
            using an RNA virus.
  JOURNAL   Plant Physiol. 2022 Jun 27;189(3):1241-1245. doi: 
            10.1093/plphys/kiac159.
  PUBMED    35389493
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Physiol"; date: "2022-06-27- 27"; volume: "189"; issue: "3"; 
            pages: "1241-1245"
COMMENT     Sequence Label: SPDK3959
FEATURES             Location/Qualifiers
     source          1..11056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2372..2447
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2986..3003
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3038..3290
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3312..3336
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4637..5266
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5695..6768
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6834..7028
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7264..7286)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7372..7512
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7427..7446
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7527..7544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7698..8286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7778..7797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9711..10505)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10930..10954
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagtctag aggacttttg ccagccatgg tgttttagag ctagaaatag caagttaaaa
     2401 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcgag ctcccgtagc
     2461 tcagttggtt agagcgttgg tcttatgagc cgaaggtcgc gggttcgagc cccgccggaa
     2521 gcacccgggc atgtcccgaa gacattaaac tacggttctt taagtagatc cgtgtctgaa
     2581 gttttaggtt caatttaaac ctacgagatt gacattctcg actgatcttg attgatcggt
     2641 aagtcttttg taatttaatt ttctttttga ttttatttta aattgttatc tgtttctgtg
     2701 tatagactgt ttgagatcgg cgtttggccg actcattgtc ttaccatagg ggaacggact
     2761 ttgtttgtgt tgttatttta tttgtatttt attaaaattc tcaacgatct gaaaaagcct
     2821 cgcggctaag agattgttgg ggggtgagta agtactttta aagtgatgat ggttacaaag
     2881 gcaaaagggg taaaacccct cgcctacgta agcgttatta cgcccgtctg tacttatatc
     2941 agtacactga cgagtcccta aaggacgaaa cgggagaacg ctagccacca ccaccaccac
     3001 cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat cgttcaaaca tttggcaata
     3061 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     3121 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     3181 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     3241 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattaaa
     3301 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     3361 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     3421 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     3481 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     3541 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     3601 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     3661 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     3721 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     3781 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     3841 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     3901 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     3961 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     4021 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     4081 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     4141 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     4201 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     4261 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     4321 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     4381 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     4441 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     4501 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     4561 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     4621 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     4681 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     4741 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     4801 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     4861 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     4921 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     4981 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     5041 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     5101 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     5161 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     5221 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     5281 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     5341 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     5401 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     5461 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     5521 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     5581 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     5641 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     5701 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     5761 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     5821 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     5881 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     5941 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     6001 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     6061 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     6121 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     6181 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     6241 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     6301 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     6361 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     6421 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     6481 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     6541 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     6601 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     6661 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     6721 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     6781 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     6841 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     6901 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     6961 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     7021 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     7081 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     7141 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     7201 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     7261 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     7321 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     7381 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     7441 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     7501 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7561 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7621 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7681 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7741 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7801 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7861 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7921 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7981 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8041 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8101 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8161 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8221 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8281 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8341 cgttaagggc tgatgaatcc cctaatgatt tttatcaaaa tcattaagtt aaggtagata
     8401 cacatcttgt catatgatca aatggtttcg ccaaaaatca ataatcagac aacaaaatgt
     8461 gcgaactcga tattttacac gactctcttt accaattctg ccccgaatta cacttaaaac
     8521 gactcaacag cttaacgttg gcttgccacg ccttacttga ctgtaaaact ctcactctta
     8581 ccgaacttgg ccgtaacctg ccaaccaaag cgagaacaaa acataacatc aaacgaatcg
     8641 accgattgtt aggtaatcgt cacctccaca aagagcgact cgctgtatac cgttggcatg
     8701 ctagctttat ctgttcgggc aatacgatgc ccattgtact tgttgactgg tctgatatcc
     8761 gtgagcaaaa acggcttatg gtattgcgag cttcagtcgc actacacggt cgttctgtta
     8821 ctctttatga gaaagcgttc ccgctttcag agcaatgttc aaagaaagct catgaccaat
     8881 ttctagccga ccttgcgagc attctaccga gtaacaccac accgctcatt gtcagtgatg
     8941 ctggctttaa agtgccatgg tataaatccg ttgagaagct gggttggtac tggttaagtc
     9001 gagtaagagg aaaagtacaa tatgcagacc taggagcgga aaactggaaa cctatcagca
     9061 acttacatga tatgtcatct agtcactcaa agactttagg ctataagagg ctgactaaaa
     9121 gcaatccaat ctcatgccaa attctattgt ataaatctcg ctctaaaggc cgaaaaaatc
     9181 agcgctcgac acggactcat tgtcaccacc cgtcacctaa aatctactca gcgtcggcaa
     9241 aggagccatg gattctagca actaacttac ctgttgaaat tcgaacaccc aaacaacttg
     9301 ttaatatcta ttcgaagcga atgcagattg aagaaacctt ccgagacttg aaaagtcctg
     9361 cctacggact aggcctacgc catagccgaa cgagcagctc agagcgtttt gatatcatgc
     9421 tgctaatcgc cctgatgctt caactaacat gttggcttgc gggcgttcat gctcagaaac
     9481 aaggttggga caagcacttc caggctaaca cagtcagaaa tcgaaacgta ctctcaacag
     9541 ttcgcttagg catggaagtt ttgcggcatt ctggctacac aataacaagg gaagactcac
     9601 tcgtggctgc aaccctgctt actcaaaatc tattcacaca tggttacgtt ttggggaaat
     9661 tatgagggga tctctcagcg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
     9721 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
     9781 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
     9841 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
     9901 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
     9961 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
    10021 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
    10081 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
    10141 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
    10201 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
    10261 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
    10321 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
    10381 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
    10441 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
    10501 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
    10561 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
    10621 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
    10681 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
    10741 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
    10801 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
    10861 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
    10921 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
    10981 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattccta ggccaccatg
    11041 ttgggcccgg cgcgcc
//
