LOCUS       sequence-396567-        3701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-Integrase13-9
FEATURES             Location/Qualifiers
     source          1..3701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             97..117
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1581..1599)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1667..1771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1993..2784
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2955..3543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3444..3463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3697..3701,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atggccccaa agaaaaagag gaaagttatg
      121 gccgtcggaa tatacatacg tgtttccact caagaacagg caagtgaagg acatagcatt
      181 gagagtcaaa agaaaaaact ggcttcctac tgcgaaatcc aaggatggga tgactaccgt
      241 ttctatattg aagaggggat tagcggtaag aatacaaata gaccaaaact aaaactacta
      301 atggaacata tagaaaaggg caaaatcaac attttattgg tttaccgact tgaccgatta
      361 accagaagcg ttatcgatct tcataagctc ctgaacttcc ttcaggaaca cgggtgtgct
      421 ttcaagagtg caacagaaac ttatgacacc acaactgcaa atgggcgtat gtcaatgggg
      481 atcgtaagcc tactagctca gtgggaaact gaaaacatgt ccgagagaat caaactaaat
      541 ttagagcaca aggttctggt ggagggtgaa cgagtgggag caatacctta tggcttcgat
      601 ctcagcgacg atgagaagct cgtcaagaac gaaaaatccg ccattctcct agatatggtc
      661 gagcgagtcg aaaacggctg gtctgttaat cgaatagtga actacttaaa cctcactaat
      721 aatgacagaa actggagtcc caatggagtt ctccgattgc ttagaaaccc tgccctatac
      781 ggggcaacca gatggaatga taaaattgcc gagaataccc atgagggaat aatttctaaa
      841 gaacgattca accgacttca gcaaatcttg gctgatcgat caatccacca ccgaagagat
      901 gtgaagggta cttacatctt ccagggggtc ctgagatgcc ccgtatgcga ccagacactc
      961 agcgtcaacc gattcatcaa aaagcgtaag gatggcaccg agtactgtgg ggtattatat
     1021 agatgccagc catgtattaa acaaaacaag tacaatttag caatcgggga agcacgtttt
     1081 ctgaaagctc taaacgagta tatgtctaca gtcgaatttc aaacagtgga agatgaggta
     1141 attcctaaga agagtgaacg tgaaatgctt gaatcacagt tacaacagat cgctcgaaaa
     1201 cgagaaaaat atcaaaaggc atgggcctct gaccttatga gcgacgacga atttgagaaa
     1261 ttaatggttg aaactcgtga aacttacgac gagtgtaaac aaaagcttga atcctgcgag
     1321 gaccctatca agatcgatga aacctaccta aaagaaattg tatacatgtt tcaccaaaca
     1381 tttaatgacc tggaaagtga gaagcaaaag gaattcatat ccaagtttat tagaacaatt
     1441 agatatacag tcaaggagca gcagcctatc cgacctgaca aatccaaaac aggcaagggc
     1501 aaacagaagg tcatcatcac tgaagtcgag ttttatcaat agctttgaga ccacgaagtg
     1561 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1621 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1681 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1741 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
     1801 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1861 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1921 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1981 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     2041 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2101 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     2161 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     2221 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2281 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     2341 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     2401 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     2461 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     2521 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     2581 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     2641 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     2701 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     2761 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     2821 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3601 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3661 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg a
//
