LOCUS       sequence-396578-        2373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 3b part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK116
FEATURES             Location/Qualifiers
     source          1..2373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      266..275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      complement(738..772)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(843..1502)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1417..1436
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1684..2272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2173..2192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctgactcta agggtgaaga agataacatg gcttctttgc cagccaccca
       61 cgagttgcac atcttcggtt ccattaacgg tgtcgacttc gatatggtcg gtcaaggtac
      121 cggtaaccca aacgacggtt acgaagaatt gaacttgaag tctaccaagg gcgacttgca
      181 gttctcccct tggattttgg ttccacacat cggttacggt ttccaccaat acttgccata
      241 cccagacggt atgtccccat tccaagccgc catggttgac ggttctggtt accaagtcca
      301 cagaaccatg caattcgaag acggtgcttc cttgaccgtg aactacagat acacctacga
      361 aggttctcac atcaagggcg aagcccaagt taagggtacc ggtttccctg ctgacggtcc
      421 agtcatgact aactccttga ccgctgctga ctggtgtcgt tccaagaaga cctacccaaa
      481 cgataagacc atcatttcca ctttcaagtg gtcctacacc accggtaacg gcaagagata
      541 ccgctctacc gctcgtacca cctacacctt cgccaagcca atggctgcca actacttgaa
      601 gaaccagcct atgtacgtct tccgtaagac cgagctgaag cattccaaga ctgaattgaa
      661 cttcaaggaa tggcaaaagg ctttcaccga cgtcatgggt atggacgaat tgtacaaggg
      721 atcctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      781 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      841 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      901 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      961 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     1021 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
     1081 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1141 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1201 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1261 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1321 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1381 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1441 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     1501 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1561 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     1621 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1681 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1741 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1801 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1861 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1921 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1981 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2041 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2101 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2161 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2221 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2281 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2341 tgcgttatcc cctgattctg tggataaccg tag
//
