LOCUS       sequence-396579-        2374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 4a part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK117
FEATURES             Location/Qualifiers
     source          1..2374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      266..275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      complement(739..773)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(844..1503)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1418..1437
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1685..2273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2174..2193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccgactcta agggtgaaga agataacatg gcttctttgc cagccaccca
       61 cgagttgcac atcttcggtt ccattaacgg tgtcgacttc gatatggtcg gtcaaggtac
      121 cggtaaccca aacgacggtt acgaagaatt gaacttgaag tctaccaagg gcgacttgca
      181 gttctcccct tggattttgg ttccacacat cggttacggt ttccaccaat acttgccata
      241 cccagacggt atgtccccat tccaagccgc catggttgac ggttctggtt accaagtcca
      301 cagaaccatg caattcgaag acggtgcttc cttgaccgtg aactacagat acacctacga
      361 aggttctcac atcaagggcg aagcccaagt taagggtacc ggtttccctg ctgacggtcc
      421 agtcatgact aactccttga ccgctgctga ctggtgtcgt tccaagaaga cctacccaaa
      481 cgataagacc atcatttcca ctttcaagtg gtcctacacc accggtaacg gcaagagata
      541 ccgctctacc gctcgtacca cctacacctt cgccaagcca atggctgcca actacttgaa
      601 gaaccagcct atgtacgtct tccgtaagac cgagctgaag cattccaaga ctgaattgaa
      661 cttcaaggaa tggcaaaagg ctttcaccga cgtcatgggt atggacgaat tgtacaagta
      721 atggctgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      781 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
      841 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      901 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      961 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     1021 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat
     1081 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     1141 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     1201 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     1261 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     1321 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     1381 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     1441 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     1501 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     1561 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa
     1621 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1681 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1741 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1801 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1861 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1921 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1981 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2041 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2101 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2161 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2221 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2281 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2341 ctgcgttatc ccctgattct gtggataacc gtag
//
