LOCUS       sequence-396583-        1723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 4a part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK128
FEATURES             Location/Qualifiers
     source          1..1723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(88..122)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(193..852)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     767..786
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1034..1622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1523..1542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccatggttt atattggtat cgctattttt ttgtttttgg ttggcctttt
       61 tatgaaatga tggctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg
      121 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      181 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
      241 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
      301 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
      361 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac
      421 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
      481 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
      541 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
      601 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
      661 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
      721 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
      781 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
      841 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
      901 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca
      961 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1021 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1081 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1141 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1201 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1261 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1321 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1381 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1441 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1501 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1561 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1621 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1681 tgttctttcc tgcgttatcc cctgattctg tggataaccg tag
//
