LOCUS       sequence-396584-        2302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 4a part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK113
FEATURES             Location/Qualifiers
     source          1..2302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(667..701)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(772..1431)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1346..1365
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1613..2201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2102..2121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tcctctaaag gtgaagaatt gtttactggt gttgttccaa ttttggttga
       61 attggatggt gatgttaatg gtcataaatt ttctgttaga ggtgaaggtg aaggtgatgc
      121 tactattggt aaattgactt tgaaatttat ttgtactact ggtaaattgc cagttccatg
      181 gccaactttg gttactactt tgacttatgg tgttcaatgt ttttctagat atccagatca
      241 tatgaaacaa catgattttt ttaaatctgc tatgccagaa ggttatgttc aagaaagaac
      301 tatttctttt aaagatgatg gtaaatataa aactagagct gttgttaaat ttgaaggtga
      361 tactttggtt aatagaattg aattgaaagg tactgatttt aaagaagatg gtaatatttt
      421 gggtcataaa ttggaatata attttaattc tcataatgtt tatattactg ctgataaaca
      481 aaaaaatggt attaaagcta attttactgt tagacataat gttgaagatg gttctgttca
      541 attggctgat cattatcaac aaaatactcc aattggtgat ggtccagttt tgttgccaga
      601 taatcattat ttgtctactc aaactgtttt gtctaaagat ccaaattaat ggctgagacc
      661 agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
      721 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
      781 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
      841 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      901 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
      961 atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa
     1021 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     1081 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     1141 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     1201 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     1261 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     1321 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     1381 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     1441 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     1501 atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg
     1561 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1621 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1681 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1741 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1801 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1861 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1921 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1981 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2041 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2101 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2161 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2221 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2281 ctgattctgt ggataaccgt ag
//
