LOCUS       sequence-396608-       15826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15826)
  AUTHORS   Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis JC, 
            Christiansen JR, Mermet-Joret N, Niculescu D, Lauritsen J, Iannielli
            A, Klaestrup IH, Jensen UB, Qvist P, Nabavi S, Broccoli V, Nykjaer 
            A, Romero-Ramos M, Denham M
  TITLE     Enhanced production of mesencephalic dopaminergic neurons from 
            lineage-restricted human undifferentiated stem cells.
  JOURNAL   Nat Commun. 2023 Dec 5;14(1):7871. doi: 10.1038/s41467-023-43471-0.
  PUBMED    38052784
REFERENCE   2  (bases 1 to 15826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-12-5"; volume: "14(1):7871. doi"; pages: " 
            10.1038/s41467-023-43471-0"
COMMENT     Sequence Label: pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..15826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2365..2398)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2403..2716
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2635..2655
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        2739..2814
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(2844..2919)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(2941..3189)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2999..3018)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3169..3189)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3219..3433
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     3354..3373
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        3463..3538
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(3568..3643)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3940..5151
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4988..5007
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5180..5189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5189..5254
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5261..5281
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5291..9394
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5291..9394
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5291..9394
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9407..9427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9434..9487
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(9494..9496,9497..10207)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9494..9496,9497..10207)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9536..9557)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9797..9816)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10144..10165
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(10223..10256)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    10281..10869
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10334..10354)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10752..10763)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10872..10888)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10873..10889)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11394..11574
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11600..11617)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11606..11830
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11876..12304
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11963..11982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12173..12194
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12313..12333)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12318..12647
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12498..12633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12560..12579
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12695..12742
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12761..13135
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13265..13386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13302..13321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13356..13375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13435..13451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13435..13451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13448..13470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13459..13475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13483..13489,13490..13507,13508..13513))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13528..13549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13666..13683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13837..14425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13917..13936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14596..15387,15388..15456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15219..15238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15457..15561)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15636..15655)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaataact tcgtatagca tacattatac gaagttatga
     2401 tagatccgac gccgccatct ctaggcccgc gccggccccc tcgcacagac ttgtgggaga
     2461 agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt aactatagag
     2521 gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta cattttacat
     2581 gataggcttg gatttctata agagatacaa atactaaatt attattttaa aaaacagcac
     2641 aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata aatatccctt
     2701 ggagaaaagc cttgtttgct acaccgacca ccaacgccgt tttagagcta gaaatagcaa
     2761 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     2821 tgtacgctca gcggagaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
     2881 actagcctta ttttaacttg ctatttctag ctctaaaacg tctctcggaa aggcccaggc
     2941 ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac
     3001 ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa
     3061 ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat
     3121 tccaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa
     3181 taggccctct tcctgcccga ccttggatcg atccgcctga acgctgacgt catcaacccg
     3241 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg
     3301 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat
     3361 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc
     3421 tgtatgagac cactctttcc caaaaggtgg aagacgaccc gagttttaga gctagaaata
     3481 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     3541 tttttggtca tcagtccgag aaaaaaagca ccgactcggt gccacttttt caagttgata
     3601 acggactagc cttattttaa cttgctattt ctagctctaa aacgccgctg gtggtccgtg
     3661 taccgaggta cccaagcggc gcacaagcta tataaacctg aaggaagtct caactttaca
     3721 cttaggtcaa gttgcttatc gtactagagc ttcagcagga aatttaacta aaatctaatt
     3781 taaccagcat agcaaatatc atttattccc aaaatgctaa agtttgagat aaacggactt
     3841 gatttccggc tgttttgaca ctatccagaa tgccttgcag atgggtgggg catgctaaat
     3901 actgcagaca attaattaac ccgtgtcggc tccagatctg gcctccgcgc cgggttttgg
     3961 cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca
     4021 gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact
     4081 cggccttaga accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta
     4141 gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg
     4201 attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg
     4261 gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc
     4321 gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc
     4381 cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc
     4441 gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc
     4501 cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg
     4561 ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt
     4621 attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac
     4681 tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag
     4741 tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg
     4801 gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc
     4861 aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg
     4921 tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt
     4981 aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa
     5041 gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag
     5101 actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg
     5161 gctgcaggtc gactctagag ccaccatgga ctacaaagac catgacggtg attataaaga
     5221 tcatgacatc gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt
     5281 gggccgcgga atggacaaga agtactccat tgggctcgat atcggcacaa acagcgtcgg
     5341 ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa
     5401 taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga
     5461 aaccgccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa
     5521 tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt
     5581 cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc
     5641 aatctttggc aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca
     5701 tctgaggaag aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc
     5761 gctggcgcat atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga
     5821 caacagcgat gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga
     5881 agagaacccg atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc
     5941 caaatcccgg cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct
     6001 gtttggtaat cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga
     6061 cctggccgaa gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa
     6121 tctgctggcc cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc
     6181 agacgccatt ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct
     6241 gagcgctagt atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc
     6301 ccttgtcaga cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa
     6361 tggctacgcc ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa
     6421 gcccatcttg gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga
     6481 tctgttgcgc aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg
     6541 cgaactgcac gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag
     6601 ggaaaagatt gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg
     6661 gggaaattcc agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa
     6721 cttcgaggaa gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa
     6781 ctttgataaa aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta
     6841 cttcacagtt tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc
     6901 agcattcctg tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg
     6961 gaaagttacc gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc
     7021 tgttgaaatc agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct
     7081 cctgaaaatc attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga
     7141 ggacattgtc ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa
     7201 aacttacgct catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac
     7261 aggatggggg cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa
     7321 gacaatcctg gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat
     7381 ccatgatgac tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg
     7441 ggacagtctt cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat
     7501 actgcagacc gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga
     7561 gaatatcgtt atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag
     7621 tagggaaagg atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa
     7681 ggaacaccca gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca
     7741 gaacggcagg gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga
     7801 cgtggatcat atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt
     7861 gacaagatcc gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa
     7921 gaaaatgaaa aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt
     7981 cgataatctg actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat
     8041 caaaaggcag cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc
     8101 acgcatgaac accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac
     8161 tctgaagtct aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga
     8221 gatcaacaat taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact
     8281 tatcaaaaaa tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga
     8341 tgttaggaaa atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt
     8401 cttttacagc aatattatga attttttcaa gaccgagatt acactggcca atggagagat
     8461 tcggaagcga ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg
     8521 tagggatttc gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa
     8581 gaccgaagta cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga
     8641 caagctgatc gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc
     8701 tacagtcgct tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact
     8761 caaaagcgtc aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa
     8821 ccccatcgac tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa
     8881 gcttcccaag tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc
     8941 gggcgagctg cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta
     9001 tctggccagc cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct
     9061 gttcgtggaa caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc
     9121 caaaagagtg atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca
     9181 cagggataag cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa
     9241 cttgggcgcg cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac
     9301 ctctacaaag gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga
     9361 aacaagaatc gacctctctc agctcggtgg agacagcagg gctgacccca agaagaagag
     9421 gaaggtggct agcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     9481 cggccctggt accgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     9541 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     9601 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     9661 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     9721 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     9781 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     9841 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     9901 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     9961 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
    10021 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
    10081 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
    10141 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
    10201 gtacaagacc ggttgataat agataacttc gtatagcata cattatacga agttatgaat
    10261 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
    10321 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    10381 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
    10441 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
    10501 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
    10561 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
    10621 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
    10681 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
    10741 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
    10801 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
    10861 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
    10921 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
    10981 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
    11041 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
    11101 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
    11161 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
    11221 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
    11281 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
    11341 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
    11401 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    11461 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    11521 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
    11581 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    11641 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    11701 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    11761 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    11821 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
    11881 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    11941 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    12001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    12061 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    12121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    12181 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    12241 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    12301 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    12361 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    12421 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    12481 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    12541 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
    12601 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
    12661 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
    12721 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
    12781 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
    12841 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
    12901 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
    12961 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
    13021 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
    13081 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
    13141 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
    13201 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
    13261 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    13321 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    13381 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
    13441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    13501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    13561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    13621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    13681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    13741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    13801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    13861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    13921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    13981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    14041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    14101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    14161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    14221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    14281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    14341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    14401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    14461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    14521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    14581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    14641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    14701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    14761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    14821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    14881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    14941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    15001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    15061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    15121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    15181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    15241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    15301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    15361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    15421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    15481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    15541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    15601 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    15661 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    15721 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    15781 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
