LOCUS       sequence-396625-        9277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0769
FEATURES             Location/Qualifiers
     source          1..9277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1316..1325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2215..2803
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2268..2288)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2686..2697)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2875..3108
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3186..3307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3223..3242)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3277..3296
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3347..3482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3502..3521)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3503..3521)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3531..3548)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3531..3547)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3540..3562)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3689..4144
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3776..3795)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3986..4007
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4170..4274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4275..4343,4344..5135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4493..4512)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5306..5894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5795..5814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6048..6065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6182..6203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6218..6223,6224..6241,6242..6248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6256..6272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6261..6283
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6280..6296
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6280..6296
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6315..6335
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6317..6335
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6363..6589
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6590..6770
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6817..6942
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7435..7668
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7853..7897
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8128..8338
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8150..8346
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8197..8332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8378..8980
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8378..8397)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8874..8894
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9023..9140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 cccggctgct gacatgactt ctttgccact cggtgtcaaa gtggaggact ccgccttcgg
     1261 caagccggcg gggggaggcg cgggccaggc ccccagcgcc gccgcggcca cggcagccgc
     1321 catgggcgcg gacgaggagg gggccaagcc caaagtgtcc ccttcgctcc tgcccttcag
     1381 cgtggaggcg ctcatggccg accacaggaa gccgggggcc aaggagagcg ccctggcgcc
     1441 ctccgagggc gtgcaggcgg cgggtggctc ggcgcagcca ctgggcgtcc cgccggggtc
     1501 gctgggagcc ccggacgcgc cctcttcgcc gcggccgctc ggccatttct cggtgggggg
     1561 actcctcaag ctgccagaag atgcgctcgt caaagccgag agccccgaga agcccgagag
     1621 gaccccgtgg atgcagagcc cccgcttctc cccgccgccg gccaggcggc tgagcccccc
     1681 agcctgcacc ctccgcaaac acaagacgaa ccgtaagccg cggacgccct tcaccaccgc
     1741 gcagctgctg gcgctggagc gcaagttccg ccagaagcag tacctgtcca tcgccgagcg
     1801 cgcggagttc tccagctcgc tcagcctcac tgagacgcag gtgaagatat ggttccagaa
     1861 ccgccgcgcc aaggcaaaga gactacaaga ggcagagctg gagaagctga agatggccgc
     1921 caagcccatg ctgccaccgg ctgccttcgg cctctccttc cctctcggcg gccccgcagc
     1981 tgtagcggcc gcggcgggtg cctcgctcta cggtgcctct ggccccttcc agcgcgccgc
     2041 gctgcctgtg gcgcccgtgg gactctacac ggcccatgtg ggctacagca tgtaccacct
     2101 gacatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     2161 ttgtggaaag gacgagccgc gacctgtgcc tgtgcggaaa cgcgttaagt cgacaatcaa
     2221 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2281 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2341 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2401 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2461 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2521 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2581 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     2641 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     2701 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     2761 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     2821 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     2881 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     2941 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3001 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3061 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     3121 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3181 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3241 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3301 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     3361 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3421 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3481 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     3541 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     3601 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3661 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     3721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3901 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3961 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4021 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4081 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4141 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4201 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4261 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4321 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4381 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4441 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4501 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4561 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4621 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4681 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4741 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4801 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4861 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4921 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4981 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5041 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5101 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5161 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5221 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5941 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6001 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6061 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6121 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6181 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6241 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6301 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6361 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     6421 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     6481 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     6541 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     6601 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6661 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6721 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     6781 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     6841 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     6901 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     6961 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7021 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7081 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7141 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7201 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7261 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7321 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7381 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7441 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7501 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     7561 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7621 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7681 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7741 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     7801 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     7861 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     7921 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     7981 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8041 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     8101 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     8161 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8221 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8281 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8341 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     8401 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     8461 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     8521 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     8581 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     8641 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     8701 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     8761 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     8821 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     8881 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     8941 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     9001 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9061 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9121 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     9181 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
     9241 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
