LOCUS       sequence-396639-       12024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the 
            clonal raider ant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Hart T, Frank DD, Lopes LE, Olivos-Cisneros L, Lacy KD, Trible W, 
            Ritger A, Valdes-Rodriguez S, Kronauer DJC
  TITLE     Sparse and stereotyped encoding implicates a core glomerulus for ant
            alarm behavior.
  JOURNAL   Cell. 2023 Jul 6;186(14):3079-3094.e17. doi: 
            10.1016/j.cell.2023.05.025. Epub 2023 Jun 14.
  PUBMED    37321218
REFERENCE   2  (bases 1 to 12024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.05.025"; journalName: "Cell"; date: "2023-07-6-
            6"; volume: "186"; issue: "14"; pages: "3079-3094.e17"
COMMENT     Sequence Label: pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..12024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1725..1963
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1910..1931
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             join(2036..2044,2045..2062,2063..2065,2066..2098,2099..2101,
                     2102..2125,2126..2152,2153..2209,2210..2215,2216..2485,
                     2486..2503,2504..2938,2939..2944,2945..3388)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      2132..2141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2422..2443
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2549..2570)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2810..2829)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    3510..3631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3601..3620
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3918..3952
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4443..4459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4443..4459)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4456..4478)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4467..4483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4491..4497,4498..4515,4516..4521))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4536..4557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4674..4691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4845..5433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4925..4944)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5604..6395,6396..6464))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6227..6246
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6465..6569)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6595..7050)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6732..6753)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6944..6963
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7177..7199
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7191..7208
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7192..7208
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   7338..7400
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        7631..8113
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        8117..8708
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             8726..9403
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(8906..8926)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     9318..9341
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    9524..9605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttccatttgc tcgcagacat atgatgcaac atgccaccca agcgcaaaac gcttaacgct
       61 gcggctgagg ctaacgctca tgccgacgga cacgccgacg gaaacgccga cggacacgtg
      121 gccaatacgg ccgcgtcctc gaataatgcg aggttcgctg atctcactaa catcgatact
      181 ccgggtctgg gacccacaac tacgaccctg ctcgtggaac cagcacgctc aaagcgtcaa
      241 cgagtgtccc gcgcatgcga ccagtgccgt gcagcccgag agaaatgcga cggaatacag
      301 cctgcgtgtt tcccgtgcgt ttcccaggga aggtcctgca cttatcaggc ttcgccgaaa
      361 aagaggggag ttcaaaccgg ttatattcgt acgctggagc tcgccctcgc ctggatgttt
      421 gaaaatgtcg cgcgttccga agatgccttg cataacctcc tcgtccgtga cgccggacaa
      481 ggatcagctc tgctcgttgg taaagattcg ccggctgccg agcgactcca tgcccgttgg
      541 gctactagcc gtgtcaataa gagcattacc cgcctcctcc gtcagttgga gctccctcct
      601 accgccacgg ctacggcctc gataatgccg cacgtgatgg agcagcctct cagtaccagc
      661 attaaccccg tcaacgaccg cttcaacggt attcccaacc ccactccgta taactccgat
      721 gcagctctcg atgctatcac tcagaccaac gattatggaa gcgtaaatac acatggtatc
      781 ctctctactt acccgccacc ggctacgcac cttaatgaag cttccgtcgc tctcgctccc
      841 ggtggcgccc ccccccgacc gcctcctccg tatgttgaca gcacgaccaa tcacccgccg
      901 taccactcga atctggttcc aatggcgaac tttggttact cgaccgttga ttacgatgcc
      961 atggttgacg atttggctag cattgaatac acggacgctg tggatgtcga cccacagttt
     1021 atgaccaatc tgggattcgt tcctggatgt aacttctccg acattaatac atacgaacag
     1081 tgatgacgtc tatcgatacc gtcgactaaa gccaaataga aaattattca gttcctggct
     1141 taagttttta aaagtgatat tatttatttg gttgtaacca accaaaagaa tgtaaataac
     1201 taatacataa ttatgttagt tttaagttag caacaaattg attttagcta tattagctac
     1261 ttggttaata aatagaatat atttatttaa agataattgc gtttttattg tcagggagtg
     1321 agtttgctta aaaactcgtt tagatccagc gctcgcggtc acgtgtcact gggtcagtgg
     1381 gtaatcgctt atcctcggat aaacaattat cctcacgggt aatcgcttat ccgctcgggt
     1441 aatcgcttat cctcgggtaa tcgcttatcc ttgggtaatc gcttatcctc ggataaacaa
     1501 ttatcctcac gggtaatcgc ttatccgctc gggtaatcgc ttatcctcgg gtaatcgctt
     1561 atccttgggt aatcgcttat cctcggataa acaattatcc tcacgggtaa tcgcttatcc
     1621 gctcgggtaa tcgcttatcc tcgggtaatc gcttatcctt tcacgttggg actcagtgag
     1681 gaggacctga attcctgcag cccgagcgga gactctagcg agcgccggag tataaataga
     1741 ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag
     1801 cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc
     1861 aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga
     1921 aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg aattgggaat
     1981 tcgttaacag atcgggggat caattcgtta acagatctgt ttaaacgctt gttcgatggg
     2041 ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
     2101 ggatctgtac gacgatgacg ataaggatct cgccaccatg gtcgactcat cacgtcgtaa
     2161 gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcac tcgagaacgt
     2221 ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttccaca tccgccacaa
     2281 catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga
     2341 cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac tttcgaaaga
     2401 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     2461 tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga gcaagggcga
     2521 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2581 caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc tgaccctgaa
     2641 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2701 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2761 gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg acgacggcaa
     2821 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2881 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacct
     2941 gccggaccaa ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga
     3001 caaggacggg gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtctctggg
     3061 gcagaacccc acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga
     3121 cggcacaatc gacttccctg agttcctgac aatgatggca agaaaaatga aatacaggga
     3181 cacggaagaa gaaattagag aagcgttcgg tgtgtttgat aaggatggca atggctacat
     3241 cagtgcagca gagcttcgcc acgtgatgac aaaccttgga gagaagttaa cagatgaaga
     3301 ggttgatgaa atgatcaggg aagcagacat cgatggggat ggtcaggtaa actacgaaga
     3361 gtttgtacaa atgatgacag cgaagtgagc ggccgcgact ctagatcata atcagccata
     3421 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3481 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3541 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3601 gtggtttgtc caaactcatc aatgtatctt agggctacgg cgcgccatct ataacaagaa
     3661 aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta
     3721 tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc
     3781 gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca
     3841 agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga
     3901 gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatcta
     3961 gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg
     4021 ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg
     4081 gaaagagttt gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac
     4141 catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc
     4201 cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa
     4261 gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat
     4321 taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat
     4381 gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgctt ggcgtaatca
     4441 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4501 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     4561 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4621 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4681 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4741 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4801 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4861 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4921 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4981 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5041 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5101 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5161 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5221 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5281 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5341 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5401 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5461 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5521 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5581 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5641 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5701 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5761 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5821 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5881 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5941 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6001 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6061 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6121 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6181 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6241 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6301 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6361 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6421 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6481 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6541 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     6601 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     6661 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     6721 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     6781 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     6841 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     6901 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     6961 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     7021 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     7081 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     7141 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     7201 cggccagtga gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac gggcagactc
     7261 gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa gatgctcgac acgctgcaga
     7321 acacgcagct agattaaccc tagaaagata atcatattgt gacgtacgtt aaagataatc
     7381 atgtgtaaaa ttgacgcatg tgttttatcg gtctgtatat cgaggtttat ttattaattt
     7441 gaatagatat taagttttat tatatttaca cttacatact aataataaat tcaacaaaca
     7501 atttatttat gtttatttat ttattaaaaa aaacaaaaac tcaaaatttc ttctataaag
     7561 taacaaaact tttatcgaat tcctgcagcc cgggggatcc aactagttgt tcgccgagct
     7621 cttacgcgcc cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca
     7681 cacggctcat aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg
     7741 gatgatgtca tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac
     7801 tcgctttacg agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg
     7861 caatatgata tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt
     7921 agaattctac ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac
     7981 tcgctttacg agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat
     8041 aaaaataaaa gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt
     8101 agaattctac gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata
     8161 aaatgaacgg atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt
     8221 ccattgtccg tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt
     8281 ttgcgcacag acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat
     8341 cccaacggcg cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga
     8401 atgcagctga tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga
     8461 ttaatgttta tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca
     8521 ttgtatgtcg gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt
     8581 ttagagggca taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg
     8641 acgttcatgt tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa
     8701 caaccaagtg acctgttcgc cgcggatggc ctcctccgag aacgtcatca ccgagttcat
     8761 gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga
     8821 gggcgagggc cgcccctacg agggccacaa caccgtgaag ctgaaggtga ccaagggcgg
     8881 ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta
     8941 cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa
     9001 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc aggactcctc
     9061 cctgcaggac ggctgcttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga
     9121 cggccccgtg atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     9181 ccgcgacggc gtgctgaagg gcgagaccca caaggccctg aagctgaagg acggcggcca
     9241 ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta
     9301 ctactacgtg gacgccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     9361 gcagtacgag cgcaccgagg gccgccacca cctgttcctg tagcggccgc gactctagat
     9421 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     9481 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     9541 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9601 actgcattct agttgtggtt tgttgttcgc acatatgtca cgtaatcagc ttttgacgag
     9661 aaacgtggaa gtgcgaggct aatgcgattc tctggagttc aattccttaa agttcaatta
     9721 aaaagttcct atttaattgt catgtgaaaa gacactttca cctcattttc tcctcaagaa
     9781 acaactaaat tacatatcgt cgatcaacaa gattggttgc ccgaacgacg gatcaaccgc
     9841 gaatcgcaat gatcgaagtt tctgattcca tttaccgcgt aatgcaaaaa aatattttca
     9901 agaattacgt acctcaacaa tgatttaaat tgtttagttt attctaatgg agtgaaacaa
     9961 gaggagtgtt tttccatttg aaataaaaaa tgatttaaat gtaattcgca agttaaatat
    10021 ttcttcgatt gaaacattcc gttccaattt ctctattaca tagttaagaa tgtccatttc
    10081 gcagtaacta tcattagtgt tccattatac cgatcgccat gtcaaagtaa ggactgccgg
    10141 cctaaataat gaaggattcg ttactatgta cacgattcat gcctgtaagc tacagcctga
    10201 cccctgtttt aagtcgatat gtgacatatt taaccgtctc gcctcctaca taggtcacac
    10261 acaacggcga gctacgctct cgaccgattt acactatttg ttttacgtca ttcgttgcat
    10321 attgcgttct atggtacgaa tcatttattt ttatcgacca agtctaactt acgtatgtga
    10381 tgatagtaca ccaaccgcga atcattcgcg aaaattcgca cggactttat ttaataaaga
    10441 acctttcatg tttccgcctc ttccgaaaaa gaagaaaaac gagataagaa ttattcaaac
    10501 tctatttttt tccgaaaaat cagttctgtc cttacatata taaaaaaaat tggtcgttac
    10561 aatttagctc aggggtgctc agagccattg cacattgcac agcaaagctt aacaaagtat
    10621 tccggacaaa ttctattcgc aagatagata tctcgtatat gtcatataca tagttttctc
    10681 gactctttat caagtatcca ttttttaatt attactgtaa ttaatttttc taaaagctat
    10741 aatcttgtta gcgatctcgc aaacgctcga tcaccaagag ctccggatta atttcaggga
    10801 aaggagtgct ttgcgtacat caatgcaacg atcctagcgg aagcttcgtt tcaccagttg
    10861 ctaaccccga ctctaggcat cttggaactt taaattgttt tcttcagcga aactttcttt
    10921 acgctgaaaa ctgcatagag cgcacgacga cgctggcggc ctgaattatt aaggggacta
    10981 tagaatcccc ctctttgtgc actcacgggg tgatgcagaa agcgacatgg cgttggcaga
    11041 gtattgcgca gcgcaacaaa gcagtctgcg cctggttacc gggccccgat tggtccacgt
    11101 ttggtaccta ccctgttgga agggggtttt ccttcgacgt gggaggtggc ggtcaggtca
    11161 caaccagggg gttgaagaag cgcaaagacc agagggtacc tgggcttcgc ccggagagac
    11221 aatcgctcaa gacaccaaag acgggtcgca aaacgactga tggcaggccg gatagttagt
    11281 ttgctcttct tcgttcttct ggtatacttg gcacgtttgc tgcaacgtaa tcgcgaggcg
    11341 ctgaaggcca catcgtaatt ccaactgcgg gattaacaaa tttctcgttg gtgatcttgg
    11401 ataaccgaag tcaccaaggt aggctctcgt tctgtcaacc ctgtttcaac agaagcactt
    11461 gccaaatgaa ataaacattc aaaggacgtt tgttaaatat tttttcgaag aaaaggatac
    11521 ttttgatcat gtgtttcaac ttcaaacact tattacaccc ttatattatc tctgaataca
    11581 taagtataac gcaacagata tctaaaagat gtgtgaagat tatttggcac atcagaaagc
    11641 cttaactgag tgtaaacatt gcactgtctg tcaacaagct aacaagcagt tgtctttgaa
    11701 agacaagact ttctgaggaa caaaagaatc tttaagtctt tgagacattg tgttagacat
    11761 ttgctcgcga atcaagatac acaatattaa ttagcgaata tgccttacgc gaagaaagac
    11821 ctatttcgga aagtttcacc ctgcaccgtt atatgcacac cttatgacac tgtggcagat
    11881 gatgcacgaa tcgcagcgcg ctttggatag cgattacgtt tacgattacg tttacgtaat
    11941 cgctatccgt aatgtcgttg acgaattagt ttgacaacct cgtacaacag agttgatggt
    12001 cgtgacagag taatgctcag cccg
//
