LOCUS       sequence-396670-        7651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for soluble EphB3 fused to IgG1 Fc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Narayanan KK, Amaya M, Tsang N, Yin R, Jays A, Broder CC, Shukla D, 
            Procko E
  TITLE     Sequence basis for selectivity of ephrin-B2 ligand for Eph receptors
            and pathogenic henipavirus G glycoproteins.
  JOURNAL   J Virol. 2023 Nov 30;97(11):e0062123. doi: 10.1128/jvi.00621-23. 
            Epub 2023 Oct 16.
  PUBMED    37931130
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2023-11-30"; volume: "97(11):e0062123. doi"; pages: " 
            10.1128/jvi.00621-23. Epub 2023 Oct 16"
COMMENT     Sequence Label: pcDNA3-EphB3-Fc
FEATURES             Location/Qualifiers
     source          1..7651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..49)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    38..262
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      308..736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(395..414)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     605..626
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(745..765)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        750..1079
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      930..1065
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     992..1011
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1146..1940
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1200..1219)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1810..1829
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2114..2235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2151..2170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2205..2224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2284..2300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2284..2300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2297..2319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2308..2324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2332..2338,2339..2356,2357..2362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2377..2398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2515..2532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2686..3271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2766..2785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3442..4233,4234..4302))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4065..4084
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4303..4407)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4482..4501)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4673..5052
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5053..5256
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5207..5227
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5301..5320
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5301..5319
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6142..6153
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
       61 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      121 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      181 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
      241 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
      301 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      361 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      421 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      481 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      541 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
      601 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
      661 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
      721 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      901 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      961 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     1021 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     1081 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     1141 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     1201 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     1261 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     1321 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     1381 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     1441 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     1501 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     1561 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     1621 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     1681 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     1741 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     1801 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     1861 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     1921 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     1981 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     2041 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     2101 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2161 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2221 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     2281 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2341 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2401 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2461 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2521 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2581 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2641 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2701 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2761 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2821 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2881 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2941 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3001 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3061 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3121 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3181 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca
     3241 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3301 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3361 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3421 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3481 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3541 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3601 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3661 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3721 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3781 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3841 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3901 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3961 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4021 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4081 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4141 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4201 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4261 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4321 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4381 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     4441 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     4501 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     4561 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     4621 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     4681 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4741 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4801 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4861 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4921 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4981 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     5041 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     5101 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     5161 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     5221 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     5281 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagcat
     5341 gccactgctg ctcttgctgc ctctgctttg ggctggagct ctggctctgg aagagaccct
     5401 catggacaca aaatgggtaa catctgagtt ggcgtggaca tctcatccag aaagtgggtg
     5461 ggaagaggtg agtggctacg atgaggccat gaatcccatc cgcacatacc aggtgtgtaa
     5521 tgtgcgcgag tcaagccaga acaactggct tcgcacgggg ttcatctggc ggcgggatgt
     5581 gcagcgggtc tacgtggagc tcaagttcac tgtgcgtgac tgcaacagca tccccaacat
     5641 ccccggctcc tgcaaggaga ccttcaacct cttctactac gaggctgaca gcgatgtggc
     5701 ctcagcctcc tcccccttct ggatggagaa cccctacgtg aaagtggaca ccattgcacc
     5761 cgatgagagc ttctcgcggc tggatgccgg ccgtgtcaac accaaggtgc gcagctttgg
     5821 gccactttcc aaggctggct tctacctggc cttccaggac cagggcgcct gcatgtcgct
     5881 catctccgtg cgcgccttct acaagaagtg tgcatccacc accgcaggct tcgcactctt
     5941 ccccgagacc ctcactgggg cggagcccac ctcgctggtc attgctcctg gcacctgcat
     6001 ccctaacgcc gtggaggtgt cggtgccact caagctctac tgcaacggcg atggggagtg
     6061 gatggtgcct gtgggtgcct gcacctgtgc caccggccat gagccagctg ccaaggagtc
     6121 ccagtgccgc ccctgtcccc ctgggagcta caaggcgaag cagggagagg ggccctgcct
     6181 cccatgtccc cccaacagcc gtaccacctc cccagccgcc agcatctgca cctgccacaa
     6241 taacttctac cgtgcagact cggactctgc ggacagtgcc tgtaccaccg tgccatctcc
     6301 accccgaggt gtgatctcca atgtgaacga aacctcactg atcctcgagt ggagtgagcc
     6361 ccgggacctg ggtggccggg atgacctcct gtacaatgtc atctgcaaga agtgccatgg
     6421 ggctggaggg gcctcagcct gctcacgctg tgatgacaac gtggagtttg tgcctcggca
     6481 gctgggcctg acggagcgcc gggtccacat cagccatctg ctggcccaca cgcgctacac
     6541 ctttgaggtg caggcggtca acggtgtctc gggcaagagc cctctgccgc ctcgttatgc
     6601 ggccgtgaat atcaccacaa accaggctgc cccgtctgaa gtgcccacac tacgcctgca
     6661 cagcagctca ggcagcagcc tcaccctatc ctgggcaccc ccagagcggc ccaacggagt
     6721 catcctggac tacgagatga agtactttga gaagagcgag ggcatcgcct ccacagtgac
     6781 cagccagatg aactccgtgc agctggacgg gcttcggcct gacgcccgct atgtggtcca
     6841 ggtccgtgcc cgcacagtag ctggctatgg gcagtacagc cgccctgccg agtttgagac
     6901 cacaagtgag agaggctctg gggcccagca gctccaggag cagcttcccc tcggatccga
     6961 caaaactcac acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt
     7021 cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg
     7081 cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg
     7141 cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
     7201 tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg
     7261 caaggtctcc aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg
     7321 gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggaggaga tgaccaagaa
     7381 ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg
     7441 ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga
     7501 cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
     7561 cgtcttctca tgctccgtga tgcacgaggc tctgcacaac cactacacgc agaagagcct
     7621 ctccctgtct ccgggtaaat gataactcga g
//
