LOCUS       sequence-396681-       13204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for myc-tagged EphB6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Narayanan KK, Amaya M, Tsang N, Yin R, Jays A, Broder CC, Shukla D, 
            Procko E
  TITLE     Sequence basis for selectivity of ephrin-B2 ligand for Eph receptors
            and pathogenic henipavirus G glycoproteins.
  JOURNAL   J Virol. 2023 Nov 30;97(11):e0062123. doi: 10.1128/jvi.00621-23. 
            Epub 2023 Oct 16.
  PUBMED    37931130
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2023-11-30"; volume: "97(11):e0062123. doi"; pages: " 
            10.1128/jvi.00621-23. Epub 2023 Oct 16"
COMMENT     Sequence Label: pCEP4-myc-EphB6
FEATURES             Location/Qualifiers
     source          1..13204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        197..576
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        577..780
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     731..751
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     777..801
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             883..912
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3881..4015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3905..3924)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3959..3978
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4158..4180)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4740..6529
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(6831..8756)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGXGXGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
                     GGGGGGGGGGGGGGGGGGGGGGXGGGXGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(9191..9209)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9277..9381
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9382..9450,9451..10242)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9600..9619)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10413..11001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10902..10921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11432..11577
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             11620..12657
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(12655..12674)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12699..12747
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat
       61 caatattggc aattagccat attagtcatt ggttatatag cataaatcaa tattggctat
      121 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
      181 atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      241 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      301 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      361 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      421 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
      481 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
      541 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
      601 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
      721 gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      781 cgtttagtga accgtcagat ctctagaagc tgggtaccag ctgctagcgc caccatgaag
      841 accatcatcg ccctgagcta catcttctgc ctggtgttcg ccgagcaaaa gctgatctca
      901 gaggaggacc tgggcagccc tggcggtgcc ggctggctca cctacccacc aggggggtgg
      961 gacgaggtga gtgttctgga cgaccagcga cgcctgactc ggacctttga ggcatgtcat
     1021 gtggcagggg cccctccagg caccgggcag gacaattggt tgcagacaca ctttgtggag
     1081 cggcgcgggg cccagagggc gcacattcga ctccacttct ctgtgcgggc atgctccagc
     1141 ctgggtgtga gcggcggcac ctgccgggag accttcaccc tttactaccg tcaggctgag
     1201 gagcccgaca gccctgacag cgtttcctcc tggcacctca aacgctggac caaggtggac
     1261 acaattgcag cagacgagag ctttccctcc tcctcctcct cctcctcctc ttcttcctct
     1321 gcagcgtggg ctgtgggacc ccacggggct gggcagcggg ctggactgca actgaacgtc
     1381 aaagagcgga gctttgggcc tctcacccaa cgcggcttct acgtggcctt ccaggacacg
     1441 ggggcctgcc tggccctggt cgctgtcagg ctcttctcct acacctgccc tgccgtgctc
     1501 cgatcctttg cttcctttcc agagacgcag gccagtgggg ctgggggggc ctccctggtg
     1561 gcagctgtgg gcacctgtgt ggctcatgca gagccagagg aggatggagt agggggccag
     1621 gcaggaggca gcccccccag gctgcactgc aacggggagg gcaagtggat ggtagctgtc
     1681 gggggctgtc gctgccagcc tggataccaa ccagcacgag gagacaaggc ctgccaagcc
     1741 tgcccacggg ggctctataa gtcttctgct gggaatgctc cctgctcacc atgccctgcc
     1801 cgcagtcacg ctcccaaccc agcagccccc gtttgcccct gcctggaggg cttctaccgg
     1861 gccagttccg acccaccaga ggccccctgc actggtcctc catcggctcc ccaggagctt
     1921 tggtttgagg tgcaaggctc agcactcatg ctacactggc gcctgcctcg ggagctgggg
     1981 ggtcgagggg acctgctctt caatgtcgtg tgcaaggagt gtgaaggccg ccaggaacct
     2041 gccagcggtg gtgggggcac ttgtcaccgc tgcagggatg aggtccactt cgaccctcgc
     2101 cagagaggcc tgactgagag ccgagtgtta gtggggggac tccgggcaca cgtaccctac
     2161 atcttagagg tgcaggctgt taatggggtg tctgagctca gccctgaccc tcctcaggct
     2221 gcagccatca atgtcagcac cagccatgaa gtgccctctg ctgtccctgt ggtgcaccag
     2281 gtgagccggg catccaacag catcacggtg tcctggccgc agcccgacca gaccaatggg
     2341 aacatcctgg actatcagct ccgctactat gaccaggcag aagacgaatc ccactccttc
     2401 accctgacca gcgagaccaa cactgccacc gtgacacagc tgagccctgg ccacatctat
     2461 ggtttccagg tgcgggcccg gactgctgcc ggccacggcc cctacggggg caaagtctat
     2521 ttccagacac ttcctcaagg ggagctgtct tcccagcttc cggaaagact ctccttggtg
     2581 atcggctcca tcctgggggc tttggccttc ctcctgctgg cagccatcac cgtgctggcg
     2641 gtcgtcttcc agcggaagcg gcgtgggact ggctacacgg agcagctgca gcaatacagc
     2701 agcccaggac tcggggtgaa gtattacatc gacccctcca cctacgagga cccctgtcag
     2761 gccatccgag aacttgcccg ggaagtcgat cctgcttata tcaagattga ggaggtcatt
     2821 gggacaggct cttttggaga agtgcgccag ggccgcctgc agccacgggg acggagggag
     2881 cagactgtgg ccatccaggc cctgtgggcc gggggcgccg aaagcctgca gatgaccttc
     2941 ctgggccggg ccgcagtgct gggtcagttc cagcacccca acatcctgcg gctggagggc
     3001 gtggtcacca agagccgacc cctcatggtg ctgacggagt tcatggagct tggccccctg
     3061 gacagcttcc tcaggcagcg ggagggccag ttcagcagcc tgcagctggt ggccatgcag
     3121 cggggagtgg ctgctgccat gcagtacctg tccagctttg ccttcgtcca tcgctcgctg
     3181 tctgcccaca gcgtgctggt gaatagccac ttggtgtgca aggtggcccg tcttggccac
     3241 agtcctcagg gcccaagttg tttgcttcgc tgggcagccc cagaggtcat tgcacatgga
     3301 aagcatacaa catccagtga tgtctggagc tttgggatac tcatgtggga agtgatgagt
     3361 tatggagaac ggccttactg ggacatgagt gagcaggagg tactaaatgc aatagagcag
     3421 gagttccggc tgcccccgcc tccaggctgt cctcctggat tacatctact tatgttggac
     3481 acttggcaga aggaccgtgc ccggcggcct cattttgacc agctggtggc tgcatttgac
     3541 aagatgatcc gcaagccaga taccctgcag gctggcgggg acccagggga aaggccttcc
     3601 caggcccttc tgacccctgt ggccctggac tttccttgtc tggactcacc ccaggcctgg
     3661 ctttcagcca ttggactgga gtgctaccag gacaacttct ccaagtttgg cctctgtacc
     3721 ttcagtgatg tggctcagct cagcctagaa gacctgcctg ccctgggcat caccctggct
     3781 ggccaccaga agaagctgct gcaccacatc cagctccttc agcaacacct gaggcagcag
     3841 ggctcagtgg aggtctgata actcgaggcc ggcaaggccg gatccagaca tgataagata
     3901 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3961 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4021 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     4081 caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc
     4141 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     4201 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     4261 cggggcgcag ccatgaggtc gactctagag gatcgatccc cgccccggac gaactaaacc
     4321 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     4381 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     4441 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     4501 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     4561 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     4621 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     4681 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     4741 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     4801 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     4861 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     4921 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     4981 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     5041 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     5101 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     5161 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     5221 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     5281 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     5341 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     5401 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     5461 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     5521 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     5581 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     5641 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     5701 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     5761 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     5821 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     5881 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     5941 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     6001 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     6061 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     6121 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     6181 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     6241 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     6301 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     6361 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     6421 gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc tttgcatatg
     6481 ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc tggttgctcc
     6541 cattcttagg tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca
     6601 ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt
     6661 gacaacatgg gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga
     6721 tggccagaaa tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg
     6781 gggaatacac ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc
     6841 ccttcctcac cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc
     6901 gcggcagccc cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag
     6961 ctgcacacag tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta
     7021 atcgcatcct tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag
     7081 acaatggact cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg
     7141 agacgactca atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg
     7201 ttgtaaaggg aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg
     7261 tcggtagtcc tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc
     7321 tcaaatttcg ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg
     7381 cgcctgcctc catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct
     7441 gcggggccct gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct
     7501 tggtggtatt caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga
     7561 gggggcctgc gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc
     7621 ttttctccac gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt
     7681 tcacgtcctc taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct
     7741 cgaccccggc ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct
     7801 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     7861 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctcctgct
     7921 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     7981 cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgctcctgcc
     8041 cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     8101 cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     8161 cctgctcctg cccctcctgc ccctcctscc cctcctccts ctcctccccc tcctccycct
     8221 cctccycctc ctcccccycc tccyccyccy cccccyccyc ccccyccycc cccycctcct
     8281 cctcctcccc ctcctccycc tccycctcct cctccycctc ctcctcctcc tcctcctcct
     8341 ccycctcctc cccctcctcc tsctcctscc cctcctcctg ctcctgcccc tcctgcccct
     8401 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct
     8461 cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg
     8521 caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag
     8581 ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca
     8641 tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg
     8701 tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt
     8761 cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg
     8821 cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga
     8881 cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac
     8941 tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca
     9001 ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc
     9061 ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct
     9121 gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga
     9181 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     9241 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9301 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9361 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9421 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9481 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     9541 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     9601 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     9661 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     9721 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     9781 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     9841 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     9901 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     9961 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10021 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10081 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10141 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10201 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10261 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    10321 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10381 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10441 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10501 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
    10561 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10621 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10681 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10741 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    10801 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    10861 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    10921 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    10981 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11041 tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
    11101 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
    11161 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg
    11221 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
    11281 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
    11341 ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
    11401 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
    11461 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca
    11521 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag
    11581 cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
    11641 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
    11701 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
    11761 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
    11821 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
    11881 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
    11941 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
    12001 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
    12061 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
    12121 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
    12181 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
    12241 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
    12301 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
    12361 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
    12421 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
    12481 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
    12541 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
    12601 agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac
    12661 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
    12721 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
    12781 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
    12841 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
    12901 gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt
    12961 atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga
    13021 gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac
    13081 gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt
    13141 tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg
    13201 ggca
//
