LOCUS       sequence-396685-       11292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 11292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6B
FEATURES             Location/Qualifiers
     source          1..11292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             909..938
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(948..950,951..953,954..1664)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(948..950,951..953,954..1664)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(993..1014)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1254..1273)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1601..1622
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6883..6900)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6889..7113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7159..7587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7246..7265)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7456..7477
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7596..7616)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7601..7930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7781..7916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7997..8791
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8051..8070)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8661..8680
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8965..9086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9002..9021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9056..9075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9135..9151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9135..9151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9148..9170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9159..9175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9183..9189,9190..9207,9208..9213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9228..9249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9366..9383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9537..10125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9617..9636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10296..11087,11088..11156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10919..10938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11157..11261)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccatgga acaaaaactc atctcagaag aggatctgtc cggcggcatg gtgagcaagg
      961 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1021 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1081 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1141 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1201 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1261 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1321 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1381 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1441 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1501 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1561 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1621 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccggc ggcaagctta
     1681 tgagagagaa ggagcaagaa agggaagaac agttaatgga agacaagaaa aggaagaaag
     1741 aggataaaaa gaaaaaagaa gccactcaga aggtcacgga acaaaaaacc aaagtgcccg
     1801 aagtgacgaa accaagttta agccaaccaa cggccgccag cccaattggc agctctccat
     1861 cgccaccagt caatggtggc aacaatgcca aaagggtggc agtgccgaac ggacaaccgc
     1921 caagcgccgc ccgctacatg cctcgggagg tgccgccgcg attccgttgc cagcaggacc
     1981 acaaagtgtt actaaaacgt gggcagcccc ctccaccgtc ctgcatgctc cttgggggtg
     2041 gggcagggcc tcctccctgc acagcacctg gagcaaaccc aaacaacgca caagtgacag
     2101 gagcgctgct gcagagtgag agtgggactg cgccagactc aacccttgga ggtgctgctg
     2161 cttcaaatta tgcaaattcc acttggggct cgggagcctc ctccaacaac ggcacctccc
     2221 ccaacccaat tcacatctgg gacaaggtga ttgtagacgg gtctgacatg gaagagtggc
     2281 cttgtattgc cagcaaagac actgaatctt cttccgaaaa caccaccgat aacaacagtg
     2341 cctcgaaccc tggctctgag aagagcactc tgccaggaag caccactagt aacaaaggaa
     2401 aagggagcca gtgccagtct gcaagttctg ggaacgaatg taatcttggg gtctggaaat
     2461 ctgaccctaa ggctaaatct gttcaatctt ccaactctac tacagagaac aacaatggac
     2521 taggaaattg gaggaatgtg agtggtcagg atagaattgg acctggctct ggcttcagca
     2581 actttaaccc aaatagcaac ccatctgcct ggccagcact ggtccaagaa ggaacttcta
     2641 ggaaaggggc attggaaaca gataatagta attccagtgc acaggttagc acagtaggtc
     2701 agacatccag ggaacagcag tcaaagatgg aaaatgcggg tgttaatttt gttgtctctg
     2761 gcagagaaca ggctcaaatt cataacactg atggaccaaa aaatggaaac actaactcct
     2821 tgaacttaag ttcaccaaac cccatggaga ataagggaat gccctttgga atgggcttgg
     2881 ggaacacctc caggagcact gatgcccctt cacaaagcac tggagatcga aagactggga
     2941 gtgttggatc ttggggtgca gctagggggc cttctggaac tgacacagtc tctggacaaa
     3001 gcaattctgg aaacaatggg aacaatggaa aagagagaga ggactcctgg aaaggagctt
     3061 ctgttcagaa atcaactggg tcaaaaaatg actcttggga caacaataac aggtctacgg
     3121 gtgggtcctg gaactttggc ccccaggact ctaatgacaa caaatggggt gaagggaaca
     3181 aaatgacatc tggggtctct cagggagaat ggaaacagcc gactgggtct gatgagttga
     3241 aaattggaga atggagtggt ccaaaccaac caaattctag cactggagca tgggacaatc
     3301 aaaagggcca ccccctccct gaaaaccaag gcaatgccca ggctccctgt tggggaagat
     3361 cttccagctc cacaggaagt gaagttggag gtcaaagcac tggaagcaac cacaaagcag
     3421 gaagtagtga cagtcataac tctggccgtc ggtcgtacag gcccacacat cctgattgtc
     3481 aggctgtctt gcagactctt ttgagccgaa ctgatttgga ccccagggtg ctctcaaaca
     3541 ctggctgggg ccaaactcaa attaagcagg acacagtgtg ggacattgaa gaggtgccaa
     3601 ggcctgaggg gaaatctgac aaaggaactg aggggtggga gagcgctgcc acacagacca
     3661 agaactcagg gggctgggga gatgcaccca gccaaagcaa tcaaatgaag tctggatggg
     3721 gggagctctc agcctctaca gagtggaaag accccaagaa cacaggaggc tggaatgact
     3781 acaagaacaa caactcttcc aactggggag gaggacgacc tgatgaaaag acaccttcct
     3841 cttggaatga gaatcccagc aaggatcagg ggtggggagg tggacgccag cccaatcaag
     3901 gatggtcttc tggaaagaat ggttgggggg aggaagtcga tcagacaaaa aacagcaatt
     3961 gggaaagttc tgcaagtaaa cctgtgtctg ggtggggtga aggagggcag aatgaaatcg
     4021 ggacttgggg taatggtggc aatgcaagcc tagcttcaaa aggtgggtgg gaggattgca
     4081 aaagatcccc agcatggaat gagacgggcc gacagcccaa ttcctggaat aaacaacacc
     4141 aacagcagca gcccccacag cagccgccgc caccacaacc agaggcttct ggttcgtggg
     4201 gaggcccacc cccaccacct ccaggcaacg ttcgaccttc caattccagc tggagcagcg
     4261 ggccacagcc tgcaacacct aaggatgagg aacccagtgg ttgggaagag ccatccccac
     4321 agtcaattag tcggaaaatg gacattgatg atggcacttc agcatgggga gaccctaaca
     4381 gttataacta caagaatgtg aatctgtggg ataagaattc ccaagggggc ccagcacctc
     4441 gagaaccaaa cctgcccacc ccaatgacca gtaaatcggc atcagattcc aaatctatgc
     4501 aagacggctg gggggagagt gacgggccag tcacaggagc tcgccatccc agctgggaag
     4561 aggaggagga tggaggagtc tggaacacca ctggctctca gggcagtgct tcctcccaca
     4621 actcagcaag ctggggacaa ggaggaaaga aacaaatgaa gtgctcactc aaaggaggaa
     4681 acaatgattc atggatgaat cctcttgcca aacagttttc aaatatggga ttgctgagtc
     4741 agactgaaga taatccaagc agcaaaatgg atttgtctgt aggaagcctt tcagataaaa
     4801 aatttgatgt ggacaagcga gcgatgaatc tcggggattt taatgatatc atgaggaagg
     4861 atcgatctgg gttccgtcca cctaattcca aagacatggg aaccacagat agtgggcctt
     4921 attttgagaa gggcggtagt catggtttgt ttggaaacag cacagcacaa tcgagaggtc
     4981 tgcacacacc cgtgcagcca ctaaattctt ctcccagtct ccgggcgcaa gtgcctcccc
     5041 agtttatttc cccccaggtt tctgcctcaa tgctcaagca gtttcccaac agtggcctga
     5101 gtccaggtct tttcaatgtg gggccccagt tatctcctca acaaattgcc atgctgagcc
     5161 agcttccaca aattccccag tttcagttgg catgtcagct tctcttgcag cagcagcaac
     5221 agcagcagtt gttacagaac cagagaaaga tttctcaagc tgtacgccaa cagcaagagc
     5281 agcagctggc tcgaatggtg agtgcactgc agcagcagca gcagcagcag cagaggcagc
     5341 caggcatgaa gcactcgccc tctcatcctg ttgggcccaa gccgcatctg gacaacatgg
     5401 tacccaacgc attgaatgtg gggctcccag accttcaaac caaagggcca atacctggat
     5461 atggttctgg cttcagctct ggcggcatgg actatggcat ggttggtggg aaggaggctg
     5521 gaaccgagtc tcgctttaaa cagtggacct ccatgatgga ggggctgccc tctgtagcca
     5581 cacaggaagc caatatgcac aaaaatggcg ctatagtggc ccctggtaaa acccggggag
     5641 ggtcaccgta caaccagttt gatatcatcc ctggtgacac actgggtggc catacgggtc
     5701 ctgctggtga tagctggtta cctgccaaat ctccaccaac aaataaaatc ggaagtaaat
     5761 ccagcaatgc cagttggcct ccagaattcc aaccaggagt gccatggaaa ggtatccaaa
     5821 acattgaccc tgaatctgac ccctatgtca ccccaggaag tgtgctgggg ggtacagcca
     5881 catctcccat tgtagatact gaccaccaac tgctgcggga taacaccaca gggtctaatt
     5941 cttccctcaa cacctcgctg ccttcacctg gtgcctggcc ctacagtgcc tctgacaact
     6001 cctttaccaa cgttcatagc acttcagcaa agttccctga ttacaaatca acatggtccc
     6061 cagatcccat aggacacaac cccactcatc tctccaacaa gatgtggaaa aaccatattt
     6121 cctccaggaa cactacaccg ctgccccgcc cacctcctgg tctgaccaac cccaaaccat
     6181 catctccctg gagcagcaca gcaccccgat cagtcagggg gtgggggaca caggactcac
     6241 ggctcgcctc ggcctctacc tggagtgatg gtggctcagt tcgtcctagt tactggctgg
     6301 ttcttcacaa tctcacccca cagattgatg ggtcaacctt gagaacgatc tgcatgcagc
     6361 atggcccact gctgacattc catctgaatc taacccaggg cactgccctg atccgataca
     6421 gcaccaaaca ggaggcggcc aaggcccaaa ctgcactgca catgtgtgtg ttgggaaaca
     6481 ctaccatcct tgctgagttt gccactgatg atgaagtcag ccgctttctg gcacaagctc
     6541 agccccctac acctgcagca accccaagtg cgccagctgc ggggtggcag tcgctggaga
     6601 ccggccagaa ccagtcagat cccgtgggac ctgctctgaa tctttttggt gggtccactg
     6661 ggctcgggca gtggagcagc agcgctggtg gcagcagcgg ggccgatctt gctggcgctt
     6721 cattgtgggg gcccccaaac tattcttcta gcttatgggg agtcccaacg gtggaagatc
     6781 cccataggat gggcagccct gctcctttac tacctggtga ccttctggga ggagggtcgg
     6841 attcaatctg atctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     6901 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6961 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7021 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7081 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7141 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7201 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7261 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7321 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7381 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7441 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7501 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7561 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     7621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7861 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7921 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     7981 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     8041 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     8101 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     8161 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     8221 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     8281 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     8341 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     8401 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     8461 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     8521 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     8581 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     8641 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     8701 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     8761 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     8821 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8881 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8941 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     9001 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9061 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     9121 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9181 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9241 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9301 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9361 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9421 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9481 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9541 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9601 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9661 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9721 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9781 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9841 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9901 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9961 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10021 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10081 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10141 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10201 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10261 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10321 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10381 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10441 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10501 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10561 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10621 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10681 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10741 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10801 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10861 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10921 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10981 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11041 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11101 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11161 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11221 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11281 ccacctgacg tc
//
