LOCUS       sequence-396701-       13180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for myc-tagged EphB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Narayanan KK, Amaya M, Tsang N, Yin R, Jays A, Broder CC, Shukla D, 
            Procko E
  TITLE     Sequence basis for selectivity of ephrin-B2 ligand for Eph receptors
            and pathogenic henipavirus G glycoproteins.
  JOURNAL   J Virol. 2023 Nov 30;97(11):e0062123. doi: 10.1128/jvi.00621-23. 
            Epub 2023 Oct 16.
  PUBMED    37931130
REFERENCE   2  (bases 1 to 13180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2023-11-30"; volume: "97(11):e0062123. doi"; pages: " 
            10.1128/jvi.00621-23. Epub 2023 Oct 16"
COMMENT     Sequence Label: pCEP4-myc-EphB1
FEATURES             Location/Qualifiers
     source          1..13180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2716..2850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2740..2759)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2794..2813
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2993..3015)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3575..5364
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5666..7591)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGXGGXGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGXGXGGGAGGAGXGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGXGGAGGAGXGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(8026..8044)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8112..8216
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8217..8285,8286..9077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8435..8454)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9248..9836
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9737..9756
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10267..10412
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10455..11492
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11490..11509)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11534..11582
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12236..12615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12616..12819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12770..12790
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12816..12840
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             12922..12951
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agatgcgctt cactgtgaga gactgcagca gcctccctaa tgtcccagga tcctgcaagg
       61 agaccttcaa cttgtattac tatgagactg actctgtcat tgccaccaag aagtcagcct
      121 tctggtctga ggccccctac ctcaaagtag acaccattgc tgcagatgag agcttctccc
      181 aggtggactt tgggggaagg ctgatgaagg taaacacaga agtcaggagc tttgggcctc
      241 ttactcggaa tggtttttac ctcgcttttc aggattatgg agcctgtatg tctcttcttt
      301 ctgtccgtgt cttcttcaaa aagtgtccca gcattgtgca aaattttgca gtgtttccag
      361 agactatgac aggggcagag agcacatctc tggtgattgc tcggggcaca tgcatcccca
      421 acgcagagga agtggacgtg cccatcaaac tctactgcaa cggggatggg gaatggatgg
      481 tgcctattgg gcgatgcacc tgcaagcctg gctatgagcc tgagaacagc gtggcatgca
      541 aggcttgccc tgcagggaca ttcaaggcca gccaggaagc tgaaggctgc tcccactgcc
      601 cctccaacag ccgctcccct gcagaggcgt ctcccatctg cacctgtcgg accggttatt
      661 accgagcgga ctttgaccct ccagaagtgg catgcactag cgtcccatca ggtccccgca
      721 atgttatctc catcgtcaat gagacgtcca tcattctgga gtggcaccct ccaagggaga
      781 caggtgggcg ggatgatgtg acctacaaca tcatctgcaa aaagtgccgg gcagaccgcc
      841 ggagctgctc ccgctgtgac gacaatgtgg agtttgtgcc caggcagctg ggcctgacgg
      901 agtgccgcgt ctccatcagc agcctgtggg cccacacccc ctacaccttt gacatccagg
      961 ccatcaatgg agtctccagc aagagtccct tccccccaca gcacgtctct gtcaacatca
     1021 ccacaaacca agccgccccc tccaccgttc ccatcatgca ccaagtcagt gccactatga
     1081 ggagcatcac cttgtcatgg ccacagccgg agcagcccaa tggcatcatc ctggactatg
     1141 agatccggta ctatgagaag gaacacaatg agttcaactc ctccatggcc aggagtcaga
     1201 ccaacacagc aaggattgat gggctgcggc ctggcatggt atatgtggta caggtgcgtg
     1261 cccgcactgt tgctggctac ggcaagttca gtggcaagat gtgcttccag actctgactg
     1321 acgatgatta caagtcagag ctgagggagc agctgcccct gattgctggc tcggcagcgg
     1381 ccggggtcgt gttcgttgtg tccttggtgg ccatctctat cgtctgtagc aggaaacggg
     1441 cttatagcaa agaggctgtg tacagcgata agctccagca ttacagcaca ggccgaggct
     1501 ccccagggat gaagatctac attgacccct tcacttatga ggatcccaac gaagctgtcc
     1561 gggagtttgc caaggagatt gatgtatctt ttgtgaaaat tgaagaggtc atcggagcag
     1621 gggagtttgg agaagtgtac aaggggcgtt tgaaactgcc aggcaagagg gaaatctacg
     1681 tggccatcaa gaccctgaag gcagggtact cggagaagca gcgtcgggac tttctgagtg
     1741 aggcgagcat catgggccag ttcgaccatc ctaacatcat tcgcctggag ggtgtggtca
     1801 ccaagagtcg gcctgtcatg atcatcacag agttcatgga gaatggtgca ttggattctt
     1861 tcctcaggca aaatgacggg cagttcaccg tgatccagct tgtgggtatg ctcaggggca
     1921 tcgctgctgg catgaagtac ctggctgaga tgaattatgt gcatcgggac ctggctgcta
     1981 ggaacattct ggtcaacagt aacctggtgt gcaaggtgtc cgactttggc ctctcccgct
     2041 acctccagga tgacacctca gatcccacct acaccagctc cttgggaggg aagatccctg
     2101 tgagatggac agctccagag gccatcgcct accgcaagtt cacttcagcc agcgacgttt
     2161 ggagctatgg gatcgtcatg tgggaagtca tgtcatttgg agagagaccc tattgggata
     2221 tgtccaacca agatgtcatc aatgccatcg agcaggacta ccggctgccc ccacccatgg
     2281 actgtccagc tgctctacac cagctcatgc tggactgttg gcagaaggac cggaacagcc
     2341 ggccccggtt tgcggagatt gtcaacaccc tagataagat gatccggaac ccggcaagtc
     2401 tcaagactgt ggcaaccatc accgccgtgc cttcccagcc cctgctcgac cgctccatcc
     2461 cagacttcac ggcctttacc accgtggatg actggctcag cgccatcaaa atggtccagt
     2521 acagggacag cttcctcact gctggcttca cctccctcca gctggtcacc cagatgacat
     2581 cagaagacct cctgagaata ggcatcacct tggcaggcca tcagaagaag atcctgaaca
     2641 gcattcattc tatgagggtc cagataagtc agtcaccaac ggcaatggca tgataactcg
     2701 aggccggcaa ggccggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     2761 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     2821 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2881 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     2941 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     3001 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     3061 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc
     3121 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     3181 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     3241 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     3301 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     3361 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     3421 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     3481 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     3541 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     3601 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     3661 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     3721 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     3781 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     3841 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     3901 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     3961 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     4021 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     4081 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     4141 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     4201 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     4261 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     4321 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     4381 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     4441 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     4501 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     4561 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     4621 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     4681 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     4741 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4801 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4861 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     4921 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     4981 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     5041 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     5101 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     5161 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     5221 ctaatctgta tccgggtagc atatgctatc ctcatgcata tacagtcagc atatgatacc
     5281 cagtagtaga gtgggagtgc tatcctttgc atatgccgcc acctcccaag ggggcgtgaa
     5341 ttttcgctgc ttgtcctttt cctgctggtt gctcccattc ttaggtgaat ttaaggaggc
     5401 caggctaaag ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa tgttttccaa
     5461 cgcgagaagg tgttgagcgc ggagctgagt gacgtgacaa catgggtatg cccaattgcc
     5521 ccatgttggg aggacgaaaa tggtgacaag acagatggcc agaaatacac caacagcacg
     5581 catgatgtct actggggatt tattctttag tgcgggggaa tacacggctt ttaatacgat
     5641 tgagggcgtc tcctaacaag ttacatcact cctgcccttc ctcaccctca tctccatcac
     5701 ctccttcatc tccgtcatct ccgtcatcac cctccgcggc agccccttcc accataggtg
     5761 gaaaccaggg aggcaaatct actccatcgt caaagctgca cacagtcacc ctgatattgc
     5821 aggtaggagc gggctttgtc ataacaaggt ccttaatcgc atccttcaaa acctcagcaa
     5881 atatatgagt ttgtaaaaag accatgaaat aacagacaat ggactccctt agcgggccag
     5941 gttgtgggcc gggtccaggg gccattccaa aggggagacg actcaatggt gtaagacgac
     6001 attgtggaat agcaagggca gttcctcgcc ttaggttgta aagggaggtc ttactacctc
     6061 catatacgaa cacaccggcg acccaagttc cttcgtcggt agtcctttct acgtgactcc
     6121 tagccaggag agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt
     6181 gaccacgatg ctttccaaac caccctcctt ttttgcgcct gcctccatca ccctgacccc
     6241 ggggtccagt gcttgggcct tctcctgggt catctgcggg gccctgctct atcgctcccg
     6301 ggggcacgtc aggctcacca tctgggccac cttcttggtg gtattcaaaa taatcggctt
     6361 cccctacagg gtggaaaaat ggccttctac ctggaggggg cctgcgcggt ggagacccgg
     6421 atgatgatga ctgactactg ggactcctgg gcctcttttc tccacgtcca cgacctctcc
     6481 ccctggctct ttcacgactt ccccccctgg ctctttcacg tcctctaccc cggcggcctc
     6541 cactacctcc tcgaccccgg cctccactac ctcctcgacc ccggcctcca ctgcctcctc
     6601 gaccccggcc tccacctcct gctcctgccc ctcctgctcc tgcccctcct cctgctcctg
     6661 cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc
     6721 ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctccts
     6781 ctcctgcccc tcctgcccct cctsctcctg cccctcctgc ccctcctgct cctgcccctc
     6841 ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc
     6901 ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc
     6961 ctgcccctcc tgcccctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     7021 ctgcccctcc tcctsctcct gcccctcctg cccctcctcc tsctcctscc cctcctcctg
     7081 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc
     7141 ctgctcctgc ccctcctcct gctcctsccc ctcctscccc tcctgcccct cctcctgctc
     7201 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctgcc cctcctcctg
     7261 ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc ccctcccgct cctgctcctg
     7321 ctcctgttcc accgtgggtc cctttgcagc caatgcaact tggacgtttt tggggtctcc
     7381 ggacaccatc tctatgtctt ggccctgatc ctgagccgcc cggggctcct ggtcttccgc
     7441 ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt atcaccccct cttctttgag
     7501 gtccactgcc gccggagcct tctggtccag atgtgtctcc cttctctcct aggccatttc
     7561 caggtcctgt acctggcccc tcgtcagaca tgattcacac taaaagagat caatagacat
     7621 ctttattaga cgacgctcag tgaatacagg gagtgcagac tcctgccccc tccaacagcc
     7681 cccccaccct catccccttc atggtcgctg tcagacagat ccaggtctga aaattcccca
     7741 tcctccgaac catcctcgtc ctcatcacca attactcgca gcccggaaaa ctcccgctga
     7801 acatcctcaa gatttgcgtc ctgagcctca agccaggcct caaattcctc gtcccccttt
     7861 ttgctggacg gtagggatgg ggattctcgg gacccctcct cttcctcttc aaggtcacca
     7921 gacagagatg ctactggggc aacggaagaa aagctgggtg cggcctgtga ggatcagctt
     7981 atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc
     8041 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     8101 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8161 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8221 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8281 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8341 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8401 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     8461 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8521 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8581 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8641 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8701 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8761 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8821 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8881 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8941 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9001 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9061 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9121 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9181 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9241 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9301 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9361 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     9421 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9481 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9541 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9601 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9661 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9721 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9781 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9841 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttgaagc tgtccctgat
     9901 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg
     9961 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga
    10021 cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc
    10081 gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt
    10141 ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt catccccgtg
    10201 gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt
    10261 tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg
    10321 cagtcggggc ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg tgtggcctcg
    10381 aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatcccg
    10441 gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
    10501 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
    10561 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
    10621 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
    10681 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
    10741 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
    10801 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
    10861 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
    10921 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
    10981 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
    11041 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
    11101 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
    11161 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
    11221 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
    11281 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
    11341 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
    11401 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
    11461 gtccggatcg ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga
    11521 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
    11581 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
    11641 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
    11701 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt
    11761 gggttaggga cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg
    11821 ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga cagacccatg gtttttggat
    11881 ggcctgggca tggaccgcat gtactggcgc gacacgaaca ccgggcgtct gtggctgcca
    11941 aacacccccg acccccaaaa accaccgcgc ggatttctgg cgtgccaagc tagtcgacca
    12001 attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag
    12061 gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca attagccata
    12121 ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
    12181 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat
    12241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
    12301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
    12361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
    12421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
    12481 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    12541 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
    12601 atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt
    12661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
    12721 caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc
    12781 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
    12841 tctagaagct gggtaccagc tgctagcgcc accatgaaga ccatcatcgc cctgagctac
    12901 atcttctgcc tggtgttcgc cgagcaaaag ctgatctcag aggaggacct gggcagccct
    12961 ggcggtgcca tggaagaaac gttaatggac accagaacgg ctactgcaga gctgggctgg
    13021 acggccaatc ctgcgtccgg gtgggaagaa gtcagtggct acgatgaaaa cctgaacacc
    13081 atccgcacct accaggtgtg caatgtcttc gagcccaacc agaacaattg gctgctcacc
    13141 accttcatca accggcgggg ggcccatcgc atctacacag
//
