LOCUS       sequence-396723-        4409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-SV40-Galpha-q ONE-GO
FEATURES             Location/Qualifiers
     source          1..4409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(789..791,792..794,795..1505)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(834..855)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1095..1114)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1442..1463
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2242..2816,2817..2819,2820..2828)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2409..2426)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2636..2655
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2839..2859
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3292..3807
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    3821..4409
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3874..3894)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4292..4303)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caaaattttc gggtttatta cagggacagc agagatccag tttatcgatg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaactcg agactagttc tagagcggcc gcggatccat gactctggag tccatcatgg
      421 cgtgctgcct gagcgaggag gccaaggaag cccggaggat caacgacgag atcgagcggc
      481 agctgcgcag ggacaagcgc gacgcccgcc gggaactcaa gctgctgctg ctggggacag
      541 gggagagtgg caagagcacc ttcatcaagc agatgaggat catccacggg tcgggctact
      601 ctgacgaaga caagcgcggc ttcaccaagc tggtgtatca gaacatcttc acggccatgc
      661 aggccatgat cagagcgatg gacacactca agatcccata caagtatgaa cacaataagg
      721 ctcatgcaca attggttcga gaggttgatg tggagaaggt gtctgctttt tcaggaggtg
      781 gcggatccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      841 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      901 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct
      961 ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc
     1021 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1081 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1141 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1201 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc
     1261 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
     1321 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1381 acaaccacta cctgagctac cagtccaaac tgagcaaaga ccccaacgag aagcgcgatc
     1441 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1501 acaagtctgg cggtggagga tccgagaatc catatgtaga tgcaataaag agcttgtgga
     1561 atgatcctgg aatccaggag tgctacgaca gacgacggga atatcagtta tctgactcta
     1621 ccaaatacta tctgaatgac ttggaccgtg tagccgaccc tgagtacatg ccgacagagc
     1681 aagacgtgct tagagttcga gtccccacta cagggatcat cgaatacccc tttgacttac
     1741 aaagtgtcat tttcagaatg gtcgatgtag ggggccaaag gtcagagaga agaaaatgga
     1801 tacactgctt tgaaaatgtc acctccatca tgtttctagt agcgcttagc gaatatgatc
     1861 aagttcttgt ggagtcagac aatgagaacc gcatggagga gagcaaagca ctctttagaa
     1921 caattatcac ctacccctgg ttccagaact cctctgtgat tctgttctta aacaagaaag
     1981 atcttctaga ggagaaaatc atgtattccc acctagtcga ctacttccca gaatatgatg
     2041 gaccccagag agatgcccag gcagctcgag aattcatcct gaaaatgttc gtggacctga
     2101 accccgacag tgacaaaatc atctactccc acttcacgtg cgccacagat accgagaaca
     2161 tccgcttcgt ctttgcagcc gtcaaggaca ccatcctgca gctgaacctg aaggagtaca
     2221 atctggtcta agcggccgcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     2281 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     2341 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     2401 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     2461 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     2521 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     2581 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     2641 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     2701 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     2761 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg
     2821 ccacaacccg taccaaagat ggggagtagc aagagcaaga cgtcgaattc cggaatcaag
     2881 cttaagtacc tggaggaccg gggcgaggtg acttttgaga agatcttctc ccagaagctg
     2941 gggtacctgc ttttccgaga cttctgcctg aagcacctgg aggaggccaa gcccttggta
     3001 gagttctacg aggagatcaa gaaatacgag aagctggaga cagaggagga gcgcctggtc
     3061 tgcagccgag agatcttcga cacgtacatc atgaaggagc tgctggcctg ctcacatcct
     3121 ttctcgaaga gcgccattga gcacgtccag ggccatctgg tgaagaagca ggtgcctccg
     3181 gatctcttcc agccatatat tgaagaaatt tgccagaacc tccgaggaga cgtgttccag
     3241 aaattcatcg agagcgataa attcacacgg ttttgccagt ggaagggatc catggtcttc
     3301 acactcgaag atttcgttgg ggactggcga cagacagccg gctacaacct ggaccaagtc
     3361 cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt aactccgatc
     3421 caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt catcatcccg
     3481 tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa ggtggtgtac
     3541 cctgtggatg atcatcactt taaggtgatc ctgcactatg gcacactggt aatcgacggg
     3601 gttacgccga acatgatcga ctatttcgga cggccgtatg aaggcatcgc cgtgttcgac
     3661 ggcaaaaaga tcactgtaac agggaccctg tggaacggca acaaaattat cgacgagcgc
     3721 ctgatcaacc ccgacggctc cctgctgttc cgagtaacca tcaacggagt gaccggctgg
     3781 cggctgtgcg aacgcattct ggcgtaaacg cgtctggaac aatcaacctc tggattacaa
     3841 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3901 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3961 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     4021 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4081 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     4141 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4201 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     4261 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4321 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4381 tcggatctcc ctttgggccg cctccccgc
//
