LOCUS       sequence-396724-        4675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-SV40-Galpha-13 ONE-GO
FEATURES             Location/Qualifiers
     source          1..4675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1107..1126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1454..1475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2292..2866,2867..2869,2870..2878)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2459..2476)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2686..2705
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2889..2909
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3558..4073
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4087..4675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4140..4160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4558..4569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caaaattttc gggtttatta cagggacagc agagatccag tttatcgatg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaactcg agactagttc tagagcggcc gcggatccat ggcggacttc ctgccgtcgc
      421 ggtccgtgct gtccgtgtgc ttccccggct gcctgctgac gagtggcgag gccgagcagc
      481 aacgcaagtc caaggagatc gacaaatgcc tgtctcggga aaagacctat gtgaagcggc
      541 tggtgaagat cctgctgctg ggcgcgggcg agagcggcaa gtccaccttc ctgaagcaga
      601 tgcggatcat ccacgggcag gacttcgacc agcgcgcgcg cgaggagttc cgccccacca
      661 tctacagcaa cgtgatcaaa ggtatgaggg tgctggttga tgctcgagag aagcttcata
      721 ttccctgggg agacaactca aaccaacaac atggagataa gatgatgtcg tttgataccc
      781 gggccccgcg gggatcaggc atggtttcaa agggagaaga gttgttcaca ggggttgtac
      841 cgattcttgt agaacttgac ggagatgtca acgggcacaa gtttagtgta agtggagaag
      901 gcgagggcga tgcgacctat ggaaaactga cccttaaatt tatttgtact acgggtaaat
      961 tgcccgttcc ctggccgaca ttggtaacta cattcggata tggtttgatg tgctttgcaa
     1021 ggtatccgga tcacatgaag cagcatgatt tcttcaagtc cgccatgccc gaaggctacg
     1081 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1141 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1201 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1261 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1321 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1381 tgctgctgcc cgacaaccac tacctgagct accagtccgc catgagcaaa gaccccaacg
     1441 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1501 tggacgagct gtacaaggga tcaggcccgc ggatggcagc ccaaggaatg gtggaaacaa
     1561 gggttttctt acaatatctt cctgctataa gagcattatg ggcagacagc ggcatacaga
     1621 atgcctatga ccggcgtcga gaatttcaac tgggtgaatc tgtaaaatat ttcctggata
     1681 acttggataa acttggagaa ccagagtata tgccaacaga acaagatatt ctgcttgcca
     1741 gaagacccac caaaggcatc catgaatacg actttgaaat aaaaaatgtt cctttcaaaa
     1801 tggttgatgt aggtggtcag agatcagaaa ggaaacgttg gtttgaatgt ttcgacagtg
     1861 tgacatcaat acttttcctt gtttcctcaa gtgaatttga ccaggtgctt atggaagatc
     1921 gactgaccaa tcgccttaca gagtctctga acatttttga aacaatcgtc aataaccggg
     1981 ttttcagcaa tgtctccata attctgttct taaacaagac agacttgctt gaggagaagg
     2041 tgcaaattgt gagcatcaaa gactatttcc tagaatttga aggggatccc cactgcttaa
     2101 gagacgtcca aaaattcctg gtggaatgtt tccggaacaa acgccgggac cagcaacaga
     2161 agcccttata ccaccacttc accactgcta tcaacacgga gaacatccgc cttgttttcc
     2221 gtgacgtgaa ggatactatt ctgcatgaca acctcaagca gcttatgcta cagtgatcta
     2281 ggcggccgcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     2341 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     2401 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     2461 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     2521 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     2581 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     2641 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     2701 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     2761 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     2821 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg
     2881 taccaaagat ggggagtagc aagagcaaga cgtcgaattc cggaatcaag cttctgatta
     2941 ttggcccaga ggaagattat gacccaggtt atttcaacaa tgagagtgac atcatcttcc
     3001 aagatcttga aaaactgaag tcacatccag cttacttggt agtttttcta cgttacatcc
     3061 tctctcaggc agaccctggc cccctgcttt tttatttgtg ttcagaagtt tatcaacaga
     3121 caaatcccaa agattcccga agtctgggga aagacatctg gaacattttc ctggagaaaa
     3181 atgcgcctct cagagtgaag atccctgaga tgttgcaggc tgaaattgac ctacgcctgc
     3241 ggaacaatga ggaccctcgc aatgtgctct gtgaagctca ggaggcagtc atgctggaaa
     3301 tccaggagca gatcaacgac tacagatcca agcgtactct gggcctgggc agcctctatg
     3361 gtgaaaatga cctgctaggc ctggatgggg accctcttcg agaacgccaa atggctgaga
     3421 agcagctggc tgcccttgga gatatcttgt ccaaatatga ggaagatcgg agtgccccca
     3481 tggactttgc tgttaatacc tttatgagcc acgctgggat ccgtcttcgg gagtctcgag
     3541 gcggatcagg cggatccatg gtcttcacac tcgaagattt cgttggggac tggcgacaga
     3601 cagccggcta caacctggac caagtccttg aacagggagg tgtgtccagt ttgtttcaga
     3661 atctcggggt gtccgtaact ccgatccaaa ggattgtcct gagcggtgaa aatgggctga
     3721 agatcgacat ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa atgggccaga
     3781 tcgaaaaaat ttttaaggtg gtgtaccctg tggatgatca tcactttaag gtgatcctgc
     3841 actatggcac actggtaatc gacggggtta cgccgaacat gatcgactat ttcggacggc
     3901 cgtatgaagg catcgccgtg ttcgacggca aaaagatcac tgtaacaggg accctgtgga
     3961 acggcaacaa aattatcgac gagcgcctga tcaaccccga cggctccctg ctgttccgag
     4021 taaccatcaa cggagtgacc ggctggcggc tgtgcgaacg cattctggcg taaacgcgtc
     4081 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4141 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4201 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4261 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4321 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4381 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4441 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     4501 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4561 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4621 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgc
//
