LOCUS       sequence-396729-        5101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-hSyn-Galpha-13 ONE-GO
FEATURES             Location/Qualifiers
     source          1..5101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    188..303
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        345..792
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(1533..1552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1880..1901
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2718..3292,3293..3295,3296..3304)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2885..2902)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3112..3131
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3315..3335
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3984..4499
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4513..5101
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4566..4586)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4984..4995)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
       61 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
      121 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
      181 tatcgccttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
      241 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
      301 ttttcgggtt tattacaggg acagcagaga tccagtttat cgatagtgca agtgggtttt
      361 aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac
      421 aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac
      481 aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc
      541 ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc
      601 ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca
      661 gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc
      721 ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc
      781 ctgagagcgc agctcgagac tagttctaga gcggccgcgg atccatggcg gacttcctgc
      841 cgtcgcggtc cgtgctgtcc gtgtgcttcc ccggctgcct gctgacgagt ggcgaggccg
      901 agcagcaacg caagtccaag gagatcgaca aatgcctgtc tcgggaaaag acctatgtga
      961 agcggctggt gaagatcctg ctgctgggcg cgggcgagag cggcaagtcc accttcctga
     1021 agcagatgcg gatcatccac gggcaggact tcgaccagcg cgcgcgcgag gagttccgcc
     1081 ccaccatcta cagcaacgtg atcaaaggta tgagggtgct ggttgatgct cgagagaagc
     1141 ttcatattcc ctggggagac aactcaaacc aacaacatgg agataagatg atgtcgtttg
     1201 atacccgggc cccgcgggga tcaggcatgg tttcaaaggg agaagagttg ttcacagggg
     1261 ttgtaccgat tcttgtagaa cttgacggag atgtcaacgg gcacaagttt agtgtaagtg
     1321 gagaaggcga gggcgatgcg acctatggaa aactgaccct taaatttatt tgtactacgg
     1381 gtaaattgcc cgttccctgg ccgacattgg taactacatt cggatatggt ttgatgtgct
     1441 ttgcaaggta tccggatcac atgaagcagc atgatttctt caagtccgcc atgcccgaag
     1501 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1561 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1621 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1681 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1741 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1801 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccatg agcaaagacc
     1861 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1921 tcggcatgga cgagctgtac aagggatcag gcccgcggat ggcagcccaa ggaatggtgg
     1981 aaacaagggt tttcttacaa tatcttcctg ctataagagc attatgggca gacagcggca
     2041 tacagaatgc ctatgaccgg cgtcgagaat ttcaactggg tgaatctgta aaatatttcc
     2101 tggataactt ggataaactt ggagaaccag agtatatgcc aacagaacaa gatattctgc
     2161 ttgccagaag acccaccaaa ggcatccatg aatacgactt tgaaataaaa aatgttcctt
     2221 tcaaaatggt tgatgtaggt ggtcagagat cagaaaggaa acgttggttt gaatgtttcg
     2281 acagtgtgac atcaatactt ttccttgttt cctcaagtga atttgaccag gtgcttatgg
     2341 aagatcgact gaccaatcgc cttacagagt ctctgaacat ttttgaaaca atcgtcaata
     2401 accgggtttt cagcaatgtc tccataattc tgttcttaaa caagacagac ttgcttgagg
     2461 agaaggtgca aattgtgagc atcaaagact atttcctaga atttgaaggg gatccccact
     2521 gcttaagaga cgtccaaaaa ttcctggtgg aatgtttccg gaacaaacgc cgggaccagc
     2581 aacagaagcc cttataccac cacttcacca ctgctatcaa cacggagaac atccgccttg
     2641 ttttccgtga cgtgaaggat actattctgc atgacaacct caagcagctt atgctacagt
     2701 gatctaggcg gccgccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     2761 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     2821 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     2881 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     2941 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     3001 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     3061 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     3121 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     3181 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     3241 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac
     3301 aacccgtacc aaagatgggg agtagcaaga gcaagacgtc gaattccgga atcaagcttc
     3361 tgattattgg cccagaggaa gattatgacc caggttattt caacaatgag agtgacatca
     3421 tcttccaaga tcttgaaaaa ctgaagtcac atccagctta cttggtagtt tttctacgtt
     3481 acatcctctc tcaggcagac cctggccccc tgctttttta tttgtgttca gaagtttatc
     3541 aacagacaaa tcccaaagat tcccgaagtc tggggaaaga catctggaac attttcctgg
     3601 agaaaaatgc gcctctcaga gtgaagatcc ctgagatgtt gcaggctgaa attgacctac
     3661 gcctgcggaa caatgaggac cctcgcaatg tgctctgtga agctcaggag gcagtcatgc
     3721 tggaaatcca ggagcagatc aacgactaca gatccaagcg tactctgggc ctgggcagcc
     3781 tctatggtga aaatgacctg ctaggcctgg atggggaccc tcttcgagaa cgccaaatgg
     3841 ctgagaagca gctggctgcc cttggagata tcttgtccaa atatgaggaa gatcggagtg
     3901 cccccatgga ctttgctgtt aataccttta tgagccacgc tgggatccgt cttcgggagt
     3961 ctcgaggcgg atcaggcgga tccatggtct tcacactcga agatttcgtt ggggactggc
     4021 gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt
     4081 ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg
     4141 ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg
     4201 gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga
     4261 tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg
     4321 gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc
     4381 tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt
     4441 tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaaa
     4501 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4561 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4621 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4681 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4741 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4801 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4861 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     4921 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4981 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5041 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5101 c
//
