LOCUS       sequence-396775-        9028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ADAR1 (p150 isoform) constitutively in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Gayet RV, Ilia K, Razavi S, Tippens ND, Lalwani MA, Zhang K, Chen 
            JX, Chen JC, Vargas-Asencio J, Collins JJ
  TITLE     Autocatalytic base editing for RNA-responsive translational control.
  JOURNAL   Nat Commun. 2023 Mar 11;14(1):1339. doi: 10.1038/s41467-023-36851-z.
  PUBMED    36906659
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-11"; volume: "14(1):1339. doi"; pages: " 
            10.1038/s41467-023-36851-z"
COMMENT     Sequence Label: pDV10_pCMV-ADAR1-p150
FEATURES             Location/Qualifiers
     source          1..9028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        162..541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        542..745
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     696..716
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     790..809
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        790..808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4546..4563)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4552..4776
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4822..5250
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4909..4928)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5119..5140
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5259..5279)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5264..5593
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5444..5579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5506..5525
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5660..6454
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5714..5733)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6324..6343
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6628..6749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6665..6684)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6719..6738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6798..6814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6798..6814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6811..6833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6822..6838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6846..6852,6853..6870,6871..6876))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6891..6912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7029..7046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7200..7788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7280..7299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7959..8750,8751..8819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8582..8601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8820..8924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8999..9018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
       61 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
      121 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
      181 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      541 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      721 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
      781 tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgcc accatgaatc
      841 cgcggcaggg gtattccctc agcggatact acacccatcc atttcaaggc tatgagcaca
      901 gacagctcag ataccagcag cctgggccag gatcttcccc cagtagtttc ctgcttaagc
      961 aaatagaatt tctcaagggg cagctcccag aagcaccggt gattggaaag cagacaccgt
     1021 cactgccacc ttccctccca ggactccggc caaggtttcc agtactactt gcctccagta
     1081 ccagaggcag gcaagtggac atcaggggtg tccccagggg cgtgcatctc ggaagtcagg
     1141 ggctccagag agggttccag catccttcac cacgtggcag gagtctgcca cagagaggtg
     1201 ttgattgcct ttcctcacat ttccaggaac tgagtatcta ccaagatcag gaacaaagga
     1261 tcttaaagtt cctggaagag cttggggaag ggaaggccac cacagcacat gatctgtctg
     1321 ggaaacttgg gactccgaag aaagaaatca atcgagtttt atactccctg gcaaagaagg
     1381 gcaagctaca gaaagaggca ggaacacccc ctttgtggaa aatcgcggtc tccactcagg
     1441 cttggaacca gcacagcgga gtggtaagac cagacggtca tagccaagga gccccaaact
     1501 cagacccgag tttggaaccg gaagacagaa actccacatc tgtctcagaa gatcttcttg
     1561 agccttttat tgcagtctca gctcaggctt ggaaccagca cagcggagtg gtaagaccag
     1621 acagtcatag ccaaggatcc ccaaactcag acccaggttt ggaacctgaa gacagcaact
     1681 ccacatctgc cttggaagat cctcttgagt ttttagacat ggccgagatc aaggagaaaa
     1741 tctgcgacta tctcttcaat gtgtctgact cctctgccct gaatttggct aaaaatattg
     1801 gccttaccaa ggcccgagat ataaatgctg tgctaattga catggaaagg cagggggatg
     1861 tctatagaca agggacaacc cctcccatat ggcatttgac agacaagaag cgagagagga
     1921 tgcaaatcaa gagaaatacg aacagtgttc ctgaaaccgc tccagctgca atccctgaga
     1981 ccaaaagaaa cgcagagttc ctcacctgta atatacccac atcaaatgcc tcaaataaca
     2041 tggtaaccac agaaaaagtg gagaatgggc aggaacctgt cataaagtta gaaaacaggc
     2101 aagaggccag accagaacca gcaagactga aaccacctgt tcattacaat ggcccctcaa
     2161 aagcagggta tgttgacttt gaaaatggcc agtgggccac agatgacatc ccagatgact
     2221 tgaatagtat ccgcgcagca ccaggtgagt ttcgagccat catggagatg ccctccttct
     2281 acagtcatgg cttgccacgg tgttcaccct acaagaaact gacagagtgc cagctgaaga
     2341 accccatcag cgggctgtta gaatatgccc agttcgctag tcaaacctgt gagttcaaca
     2401 tgatagagca gagtggacca ccccatgaac ctcgatttaa attccaggtt gtcatcaatg
     2461 gccgagagtt tcccccagct gaagctggaa gcaagaaagt ggccaagcag gatgcagcta
     2521 tgaaagccat gacaattctg ctagaggaag ccaaagccaa ggacagtgga aaatcagaag
     2581 aatcatccca ctattccaca gagaaagaat cagagaagac tgcagagtcc cagaccccca
     2641 ccccttcagc cacatccttc ttttctggga agagccccgt caccacactg cttgagtgta
     2701 tgcacaaatt ggggaactcc tgcgaattcc gtctcctgtc caaagaaggc cctgcccatg
     2761 aacccaagtt ccaatactgt gttgcagtgg gagcccaaac tttccccagt gtgagtgctc
     2821 ccagcaagaa agtggcaaag cagatggccg cagaggaagc catgaaggcc ctgcatgggg
     2881 aggcgaccaa ctccatggct tctgataacc agcctgaagg tatgatctca gagtcacttg
     2941 ataacttgga atccatgatg cccaacaagg tcaggaagat tggcgagctc gtgagatacc
     3001 tgaacaccaa ccctgtgggt ggccttttgg agtacgcccg ctcccatggc tttgctgctg
     3061 aattcaagtt ggtcgaccag tccggacctc ctcacgagcc caagttcgtt taccaagcaa
     3121 aagttggggg tcgctggttc ccagccgtct gcgcacacag caagaagcaa ggcaagcagg
     3181 aagcagcaga tgcggctctc cgtgtcttga ttggggagaa cgagaaggca gaacgcatgg
     3241 gtttcacaga ggtaacccca gtgacagggg ccagtctcag aagaactatg ctcctcctct
     3301 caaggtcccc agaagcacag ccaaagacac tccctctcac tggcagcacc ttccatgacc
     3361 agatagccat gctgagccac cggtgcttca acactctgac taacagcttc cagccctcct
     3421 tgctcggccg caagattctg gccgccatca ttatgaaaaa agactctgag gacatgggtg
     3481 tcgtcgtcag cttgggaaca gggaatcgct gtgtaaaagg agattctctc agcctaaaag
     3541 gagaaactgt caatgactgc catgcagaaa taatctcccg gagaggcttc atcaggtttc
     3601 tctacagtga gttaatgaaa tacaactccc agactgcgaa ggatagtata tttgaacctg
     3661 ctaagggagg agaaaagctc caaataaaaa agactgtgtc attccatctg tatatcagca
     3721 ctgctccgtg tggagatggc gccctctttg acaagtcctg cagcgaccgt gctatggaaa
     3781 gcacagaatc ccgccactac cctgtcttcg agaatcccaa acaaggaaag ctccgcacca
     3841 aggtggagaa cggagaaggc acaatccctg tggaatccag tgacattgtg cctacgtggg
     3901 atggcattcg gctcggggag agactccgta ccatgtcctg tagtgacaaa atcctacgct
     3961 ggaacgtgct gggcctgcaa ggggcactgt tgacccactt cctgcagccc atttatctca
     4021 aatctgtcac attgggttac cttttcagcc aagggcatct gacccgtgct atttgctgtc
     4081 gtgtgacaag agatgggagt gcatttgagg atggactacg acatcccttt attgtcaacc
     4141 accccaaggt tggcagagtc agcatatatg attccaaaag gcaatccggg aagactaagg
     4201 agacaagcgt caactggtgt ctggctgatg gctatgacct ggagatcctg gacggtacca
     4261 gaggcactgt ggatgggcca cggaatgaat tgtcccgggt ctccaaaaag aacatttttc
     4321 ttctatttaa gaagctctgc tccttccgtt accgcaggga tctactgaga ctctcctatg
     4381 gtgaggccaa gaaagctgcc cgtgactacg agacggccaa gaactacttc aaaaaaggcc
     4441 tgaaggatat gggctatggg aactggatta gcaaacccca ggaggaaaag aacttttatc
     4501 tctgcccagt atagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     4561 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4621 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4681 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4741 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     4801 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4861 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4921 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4981 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5041 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5101 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5161 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5221 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     5281 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5341 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5461 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5521 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5581 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     5641 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5701 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5761 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5821 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5881 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5941 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6001 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6061 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6121 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6181 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6241 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6301 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6361 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6421 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     6481 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     6541 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6601 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     6661 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6721 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6781 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6841 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6901 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6961 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7021 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7081 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7141 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7201 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7261 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7321 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7381 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7441 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7501 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7561 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7621 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7681 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7741 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7801 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7861 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7921 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7981 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8041 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8101 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8161 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8221 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8281 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8341 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8401 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8461 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8521 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8581 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8641 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8701 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8761 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8821 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8881 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8941 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     9001 caatctgctc tgatgccgca tagttaag
//
