LOCUS       sequence-396781-       12421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pVS1-based T-DNA binary vector and destination vector for Gateway 
            cloning with NPTII as a plant selectable marker gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Lee K, Kang M, Ji Q, Grosic S, Wang K
  TITLE     New T-DNA binary vectors with NptII selection and RUBY reporter for 
            efficient maize transformation and targeted mutagenesis.
  JOURNAL   Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231.
  PUBMED    37070560
REFERENCE   2  (bases 1 to 12421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231."
COMMENT     Sequence Label: pCBL101gw
FEATURES             Location/Qualifiers
     source          1..12421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    183..204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(219..224,225..242,243..249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     262..284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     281..297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    327..451
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(476..481,482..499,500..506)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             560..1240
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(626..645)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1560..1865
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1744..1763
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1906..2030)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2098..2114)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2178..4172
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    4201..4248
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4268..5062
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4322..4341)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4932..4951
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5629..5646)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5629..5645)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5638..5660)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5860..5884
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6405..7196
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7442..8030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7931..7950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8184..8201
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8216..8356
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8282..8301)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8442..8464
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8700..8894
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8960..10033)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10462..11091)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      301 attacgaatt cgcgccaagc tatcaaacaa gtttgtacaa aaaagctgaa cgagaaacgt
      361 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
      421 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac
      481 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
      541 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      601 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      661 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      721 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
      781 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      841 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      901 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      961 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     1021 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     1081 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     1141 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     1201 gtggcaggcg gggcgtaatc tagaggatcc ggcttactaa aagccagata acagtatgcg
     1261 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     1321 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     1381 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     1441 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     1501 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     1561 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     1621 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     1681 tgctgtcaga taaagtcccc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     1741 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     1801 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     1861 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     1921 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     1981 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tcgataattc
     2041 ttaattaact agttctagag cggccgccac cgcggtggag ctcgaattcg taatcatgtc
     2101 atagctgttt cctgtgtgga tcctctagag tcgacctgca ggcatgcaat agttccggac
     2161 ttaccggcgc cgctagcctg cagtgcagcg tgacccggtc gtgcccctct ctagagataa
     2221 tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt
     2281 gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga ataatataat
     2341 ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg
     2401 gtctaaagga caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg
     2461 catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat
     2521 tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat
     2581 ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt
     2641 atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc
     2701 ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc
     2761 ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc
     2821 gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag
     2881 ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg
     2941 cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg
     3001 gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca
     3061 ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca
     3121 gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc
     3181 ccccccctct ctaccttctc tagatcggcg ttccggtcca tgcatggtta gggcccggta
     3241 gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctgctagc
     3301 gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt
     3361 tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat
     3421 tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg
     3481 tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg
     3541 tctggttggg cggtcgttct agatcggagt agaattctgt ttcaaactac ctggtggatt
     3601 tattaatttt ggatctgtat gtgtgtgcca tacatattca tagttacgaa ttgaagatga
     3661 tggatggaaa tatcgatcta ggataggtat acatgttgat gcgggtttta ctgatgcata
     3721 tacagagatg ctttttgttc gcttggttgt gatgatgtgg tgtggttggg cggtcgttca
     3781 ttcgttctag atcggagtag aatactgttt caaactacct ggtgtattta ttaattttgg
     3841 aactgtatgt gtgtgtcata catcttcata gttacgagtt taagatggat ggaaatatcg
     3901 atctaggata ggtatacatg ttgatgtggg ttttactgat gcatatacat gatggcatat
     3961 gcagcatcta ttcatatgct ctaaccttga gtacctatct attataataa acaagtatgt
     4021 tttataatta ttttgatctt gatatacttg gatgatggca tatgcagcag ctatatgtgg
     4081 atttttttag ccctgccttc atacgctatt tatttgcttg gtactgtttc ttttgtcgat
     4141 gctcaccctg ttgtttggtg ttacttctgc aggtcgactc tagaggatca attcgctagc
     4201 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag atccaccggg
     4261 atccaccatg gttgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4321 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4381 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4441 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4501 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4561 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4621 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4681 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4741 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     4801 gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4861 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4921 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4981 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5041 ccttcttgac gagttcttct gaggatccac catggttaac ctagacttgt ccatcttctg
     5101 gattggccaa cttaattaat gtatgaaata aaaggatgca cacatagtga catgctaatc
     5161 actataatgt gggcatcaaa gttgtgtgtt atgtgtaatt actagttatc tgaataaaag
     5221 agaaagagat catccatatt tcttatccta aatgaatgtc acgtgtcttt ataattcttt
     5281 gatgaaccag atgcatttca ttaaccaaat ccatatacat ataaatatta atcatatata
     5341 attaatatca attgggttag caaaacaaat ctagtctagg tgtgttttgc aagcttgcat
     5401 gcctgcagtc gtctattgga ccgacagttg ctattaatat aatgggccac catagtagac
     5461 tgacaaataa attacctgac aacatcgttt cacaaaaaaa caaacacaaa aagggagtgc
     5521 attttccagg gcatttttgt aataaaaaac agttaaaagg gagtgcaata gaaatatagg
     5581 ggtgtggaaa tagtgatttg agcacgtctt gaagcgaatt agcttggcac tggccgtcgt
     5641 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     5701 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     5761 gttgcgcagc ctgaatggcg aatgctagag caattcggcg ttaattcagt acattaaaaa
     5821 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     5881 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     5941 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     6001 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     6061 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     6121 caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     6181 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     6241 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     6301 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     6361 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
     6421 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     6481 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     6541 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     6601 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     6661 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     6721 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     6781 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     6841 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     6901 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     6961 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     7021 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     7081 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     7141 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     7201 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     7261 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     7321 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc
     7381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7561 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8101 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8161 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     8221 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     8281 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     8341 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     8401 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     8461 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     8521 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     8581 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     8641 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     8701 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     8761 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     8821 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     8881 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     8941 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     9001 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     9061 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     9121 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     9181 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     9241 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     9301 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     9361 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     9421 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     9481 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     9541 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     9601 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     9661 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     9721 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     9781 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     9841 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     9901 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     9961 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
    10021 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
    10081 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
    10141 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
    10201 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
    10261 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
    10321 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
    10381 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
    10441 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
    10501 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
    10561 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
    10621 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
    10681 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
    10741 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    10801 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    10861 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    10921 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    10981 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    11041 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    11101 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    11161 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    11221 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    11281 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    11341 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    11401 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    11461 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    11521 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    11581 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    11641 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    11701 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    11761 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    11821 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    11881 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    11941 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    12001 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    12061 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    12121 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    12181 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    12241 ctgcactata gcagcggagg ggttggatca aagtacttta aagtacttta aagtacttta
    12301 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
    12361 ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta
    12421 g
//
