LOCUS       sequence-396787-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for ADF neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH76 - [ADFp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1905..1921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1905..1921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1918..1940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2093..2681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2173..2192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2798..2822)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2922..3713,3714..3782))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3545..3564
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3783..3887)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3986..4008
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4000..4017
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4001..4017
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4641..4663,1..11))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tacgaagtta tgatatcaaa aatgattacg ccaagctgta agtttaaaca tgatcttact
       61 aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca ctcacaacga
      121 tggatacgct aatgcctcac tggtatatct aacatttttt gttttcaggg tgttatatac
      181 attataagat cagtagtata ttaactttaa accactgaat ttcttctagt tttttgtttc
      241 cagctcaagt gctcctgtac gcaaaacatt aggcttcttt gttcaaaaaa acattttctc
      301 accacctcat gacatttttt ttctcatttt tcttattacg aatgtagcct tattagcata
      361 tagcatagag gtacccaaaa ctagtttttt tttgttttga aatatatatt tgcacctctt
      421 ttttcttttt gcaaaaagag accaaaaatg gggccctcta agggcgaaga attgttcacg
      481 ggagtagtcc caatcttagt cgagcttgac ggaggtgagt tttattatcg attttcggga
      541 ttttctactt gtaattacct tttgttttta ataagttgct tttttccaga tgttaatggc
      601 cacaagtttt ccgtgtctgg agaaggagaa ggcgacgcta cctacggtaa gctgaccctg
      661 aagttcattt gcactactgg caagttacca gtaccatggc cgaccctggt gacaacattt
      721 tgctacgggg ttcaatgctt ctccagatac ccaggtaggt tttcagtttc aatttttgtt
      781 gcaactacct caactctatg ttttcagatc atatgaaaag acacgacttt tttaaatccg
      841 ccatgccaga gggttacgtt caagaacgta ccattttttt taaggacgat ggtaactaca
      901 agacacgtgc ggaggttaaa tttgaaggcg atactctcgt gaaccgtatt gagctcaagg
      961 taaatttttc aagcttctaa attccaaaat aaaccatttt aatttcttaa ttccagggaa
     1021 tcgatttcaa ggaagatgga aacattttgg gccacaagct tgaatataat tataatagtc
     1081 ataatgtcta cattatggcc gacaaacaaa aaaacggtat taaggttaac ttcaagattc
     1141 gccacaatat cgaagatgga tctgtccaac ttgctgacca ctaccaacaa aatactccaa
     1201 ttggagatgg accggttttg cttccagata accactacct ttccactcag tctgccctct
     1261 ctaaggatcc gaatgaaaaa cgtgaccata tggttctttt ggagttcgtt accgcagcag
     1321 gaattactca cggaatggat gaactgtaca agcccgggtg atggtctctg agctatgcaa
     1381 aatcctttca agcattccct tcttctctat cactcttctt tctttttgtc aaaaaattct
     1441 ctcgctaatt tatttgcttt tttaatgtta ttattttatg actttttata gtcactgaaa
     1501 agtttgcatc tgagtgaagt gaatgctatc aaaatgtgat tctgatatcc aggaaacgga
     1561 gaatctgtgc ggcaatgtct tatcatttga aacgcgccgt atcgatgctg acatcatgcg
     1621 gctcccgtgt tagtcgagaa gatgggtacg ggcctttatt catccaacac tctacggctt
     1681 cttcaagagc tagtagggca ccctgtagtt ggaaagggat tatttcgtat ggcgagccca
     1741 taccgtcttt cttgcggaag cttaacacga taggaagttg gatagtttcg aacgatggtt
     1801 ccatacggaa cgctgtctca aggatgagtc tgacggagtg taactcgaag gctaggtgga
     1861 ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata gctgtttcct
     1921 gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc gacaatctcc
     1981 aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag aacatgtgag
     2041 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2101 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2161 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2221 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2281 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2341 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2401 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2461 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2521 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2581 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2641 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2701 ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg
     2761 tagggggctt caaatcgtcc tcgtgatacc aattcggagc ctgctttttt gtacaaactt
     2821 gttgataatg gcaattcaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2881 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2941 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3001 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3061 cgagagccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3121 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3181 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3241 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3301 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3361 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3421 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3481 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3541 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3601 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3661 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3721 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3781 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3841 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3901 aaagtgccag atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     3961 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     4021 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgaccta agtgcttttc
     4081 gagatcgcca aaccggagaa cgatcaggag aatgatgagt cgccacagga gtcggcaaga
     4141 tacgtgcgtt atagatttgc tccaaacttt ttgaaactca aaacggtcgg agcaactgtg
     4201 gaattcaaag taggagacat cccaatccat catttccgaa tgatcgaacg tcacttcttc
     4261 aaagatcgcc ttctgaagtg ttttgacttt gaattcggat tctgtattcc gaattcacga
     4321 aacaactgtg aacatatcta tgagttccct caactctctc aacaactcag tgagtcatta
     4381 ttctaaaaag tacaattcaa aagactaatc tctttttcag tggacgacat gatcaacaat
     4441 ccaaacgaga ctcgttctga cagcttctat ttcgtcgata acaaactcgt catgcacaac
     4501 aaagccgact actcatatga tgcataaatc ttcattgttg agtttatctt gttgattttt
     4561 gaataaatta tcaactcttt actttttaat gggttatgaa ataaataaac attgaaaact
     4621 gataaacaac gttcatctct ataacttcgt atagcataca tta
//
