LOCUS       sequence-396791-        8143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ADAR1 (p110 isoform) constitutively in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Gayet RV, Ilia K, Razavi S, Tippens ND, Lalwani MA, Zhang K, Chen 
            JX, Chen JC, Vargas-Asencio J, Collins JJ
  TITLE     Autocatalytic base editing for RNA-responsive translational control.
  JOURNAL   Nat Commun. 2023 Mar 11;14(1):1339. doi: 10.1038/s41467-023-36851-z.
  PUBMED    36906659
REFERENCE   2  (bases 1 to 8143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-11"; volume: "14(1):1339. doi"; pages: " 
            10.1038/s41467-023-36851-z"
COMMENT     Sequence Label: pDV09_pCMV-ADAR1-p110
FEATURES             Location/Qualifiers
     source          1..8143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        162..541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        542..745
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     696..716
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     790..809
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        790..808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      828..837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3661..3678)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3667..3891
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3937..4365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4024..4043)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4234..4255
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4374..4394)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4379..4708
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4559..4694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4621..4640
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4775..5569
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4829..4848)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5439..5458
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5743..5864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5780..5799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5834..5853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5913..5929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5913..5929)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5926..5948)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5937..5953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5961..5967,5968..5985,5986..5991))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6006..6027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6144..6161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6315..6903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6395..6414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7074..7865,7866..7934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7697..7716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7935..8039)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8114..8133)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
       61 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
      121 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
      181 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      541 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      721 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
      781 tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgcc accatggccg
      841 agatcaagga gaaaatctgc gactatctct tcaatgtgtc tgactcctct gccctgaatt
      901 tggctaaaaa tattggcctt accaaggccc gagatataaa tgctgtgcta attgacatgg
      961 aaaggcaggg ggatgtctat agacaaggga caacccctcc catatggcat ttgacagaca
     1021 agaagcgaga gaggatgcaa atcaagagaa atacgaacag tgttcctgaa accgctccag
     1081 ctgcaatccc tgagaccaaa agaaacgcag agttcctcac ctgtaatata cccacatcaa
     1141 atgcctcaaa taacatggta accacagaaa aagtggagaa tgggcaggaa cctgtcataa
     1201 agttagaaaa caggcaagag gccagaccag aaccagcaag actgaaacca cctgttcatt
     1261 acaatggccc ctcaaaagca gggtatgttg actttgaaaa tggccagtgg gccacagatg
     1321 acatcccaga tgacttgaat agtatccgcg cagcaccagg tgagtttcga gccatcatgg
     1381 agatgccctc cttctacagt catggcttgc cacggtgttc accctacaag aaactgacag
     1441 agtgccagct gaagaacccc atcagcgggc tgttagaata tgcccagttc gctagtcaaa
     1501 cctgtgagtt caacatgata gagcagagtg gaccacccca tgaacctcga tttaaattcc
     1561 aggttgtcat caatggccga gagtttcccc cagctgaagc tggaagcaag aaagtggcca
     1621 agcaggatgc agctatgaaa gccatgacaa ttctgctaga ggaagccaaa gccaaggaca
     1681 gtggaaaatc agaagaatca tcccactatt ccacagagaa agaatcagag aagactgcag
     1741 agtcccagac ccccacccct tcagccacat ccttcttttc tgggaagagc cccgtcacca
     1801 cactgcttga gtgtatgcac aaattgggga actcctgcga attccgtctc ctgtccaaag
     1861 aaggccctgc ccatgaaccc aagttccaat actgtgttgc agtgggagcc caaactttcc
     1921 ccagtgtgag tgctcccagc aagaaagtgg caaagcagat ggccgcagag gaagccatga
     1981 aggccctgca tggggaggcg accaactcca tggcttctga taaccagcct gaaggtatga
     2041 tctcagagtc acttgataac ttggaatcca tgatgcccaa caaggtcagg aagattggcg
     2101 agctcgtgag atacctgaac accaaccctg tgggtggcct tttggagtac gcccgctccc
     2161 atggctttgc tgctgaattc aagttggtcg accagtccgg acctcctcac gagcccaagt
     2221 tcgtttacca agcaaaagtt gggggtcgct ggttcccagc cgtctgcgca cacagcaaga
     2281 agcaaggcaa gcaggaagca gcagatgcgg ctctccgtgt cttgattggg gagaacgaga
     2341 aggcagaacg catgggtttc acagaggtaa ccccagtgac aggggccagt ctcagaagaa
     2401 ctatgctcct cctctcaagg tccccagaag cacagccaaa gacactccct ctcactggca
     2461 gcaccttcca tgaccagata gccatgctga gccaccggtg cttcaacact ctgactaaca
     2521 gcttccagcc ctccttgctc ggccgcaaga ttctggccgc catcattatg aaaaaagact
     2581 ctgaggacat gggtgtcgtc gtcagcttgg gaacagggaa tcgctgtgta aaaggagatt
     2641 ctctcagcct aaaaggagaa actgtcaatg actgccatgc agaaataatc tcccggagag
     2701 gtttcatcag gtttctctac agtgagttaa tgaaatacaa ctcccagact gcgaaggata
     2761 gtatatttga acctgctaag ggaggagaaa agctccaaat aaaaaagact gtgtcattcc
     2821 atctgtatat cagcactgct ccgtgtggag atggcgccct ctttgacaag tcctgcagcg
     2881 accgtgctat ggaaagcaca gaatcccgcc actaccctgt cttcgagaat cccaaacaag
     2941 gaaagctccg caccaaggtg gagaacggag aaggcacaat ccctgtggaa tccagtgaca
     3001 ttgtgcctac gtgggatggc attcggctcg gggagagact ccgtaccatg tcctgtagtg
     3061 acaaaatcct acgctggaac gtgctgggcc tgcaaggggc actgttgacc cacttcctgc
     3121 agcccattta tctcaaatct gtcacattgg gttacctttt cagccaaggg catctgaccc
     3181 gtgctatttg ctgtcgtgtg acaagagatg ggagtgcatt tgaggatgga ctacgacatc
     3241 cctttattgt caaccacccc aaggttggca gagtcagcat atatgattcc aaaaggcaat
     3301 ccgggaagac taaggagaca agcgtcaact ggtgtctggc tgatggctat gacctggaga
     3361 tcctggacgg taccagaggc actgtggatg ggccacggaa tgaattgtcc cgggtctcca
     3421 aaaagaacat ttttcttcta tttaagaagc tctgctcctt ccgttaccgc agggatctac
     3481 tgagactctc ctatggtgag gccaagaaag ctgcccgtga ctacgagacg gccaagaact
     3541 acttcaaaaa aggcctgaag gatatgggct atgggaactg gattagcaaa ccccaggagg
     3601 aaaagaactt ttatctctgc ccagtatagt ctagagggcc cgtttaaacc cgctgatcag
     3661 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     3721 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     3781 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     3841 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     3901 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     3961 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4021 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4081 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4141 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4201 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4261 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4321 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     4381 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4441 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4501 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4561 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4621 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     4681 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     4741 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     4801 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4861 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4921 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     4981 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5041 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5101 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5161 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5221 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5281 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5341 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5401 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5461 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5521 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     5581 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     5641 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     5701 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     5761 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5821 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     5881 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5941 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6001 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6061 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6121 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6721 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7201 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8041 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     8101 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aag
//
