LOCUS       sequence-396795-        5287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP 
            constitutively. Expresses mNeonGreen upon target detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Gayet RV, Ilia K, Razavi S, Tippens ND, Lalwani MA, Zhang K, Chen 
            JX, Chen JC, Vargas-Asencio J, Collins JJ
  TITLE     Autocatalytic base editing for RNA-responsive translational control.
  JOURNAL   Nat Commun. 2023 Mar 11;14(1):1339. doi: 10.1038/s41467-023-36851-z.
  PUBMED    36906659
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-11"; volume: "14(1):1339. doi"; pages: " 
            10.1038/s41467-023-36851-z"
COMMENT     Sequence Label: pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..5287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        348..559
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          560..1498
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1455..1475
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2246..2302
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2366..2419
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2681..2690
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3195..3243
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3520..4108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3600..3619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4279..5070,5071..5139))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4902..4921
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5140..5244)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcgttttta tgcttgtagt attgtataat gtttttaaga tccggagaga gcgagattcc
       61 gtctcaaaga aaaaaaaagt aatgaaatga ataaaatgag tcctagagcc agtaaatgtc
      121 gtaaatgtct cagctagtca ggtagtaaaa ggtctcaact aggcagtggc agagcaggat
      181 tcaaattcag ggctgttgtg atgcctccgc agactctgag cgccacctgg tggtaatttg
      241 tctgtgcctc ttctgacgtg gaagaacagc aactaacaca ctaacacggc atttactatg
      301 ggccagccat tgttctaata ctttaattgg ctccggtgcc cgtcagtggg cagagcgcac
      361 atcgcccaca gtccccgaga agttgtgggg aggggtcggc aattgaaccg gtgcctagag
      421 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
      481 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
      541 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
      601 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
      661 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
      721 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
      781 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
      841 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
      901 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
      961 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     1021 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     1081 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     1141 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     1201 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     1261 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     1321 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     1381 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     1441 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     1501 ggatcccaag ggcagataag tttgtacaaa aaagagaggt gccaccatga gcgagctgat
     1561 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     1621 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     1681 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     1741 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     1801 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     1861 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     1921 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     1981 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     2041 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     2101 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     2161 caacaacgag acatacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     2221 tagcaaactg gggcacggta gcggcgcaac aaacttctct cttctgaaac aggcaggaga
     2281 tgtggaagaa aatccaggcc caggatccgg tttaattaag ggcctgcagg gtggaaagct
     2341 ttctactcga ggggctagcc agggagaggg cagaggaagt ctgcttacat gcggtgatgt
     2401 cgaagagaat cctggcccag gtaccatggt gtccaagggc gaagaggaca acatggccag
     2461 cctgcctgcc acccacgagc tgcacatctt cggcagcatc aacggcgtgg acttcgacat
     2521 ggtgggacag ggcaccggca accccaacga cggatacgag gaactgaacc tgaagtccac
     2581 aaagggcgac ctgcagttca gcccctggat cctggtgccc cacatcggct acggcttcca
     2641 ccagtacctg ccctaccctg acggcatgag ccctttccag gccgccatgg tggatggctc
     2701 tggctaccag gtgcacagaa ccatgcagtt cgaggacggc gccagcctga ccgtgaacta
     2761 cagatacacc tacgagggca gccacatcaa gggcgaggcc caagtgaagg gcacaggctt
     2821 ccctgctgac ggccccgtga tgaccaactc tctgacagcc gccgactggt gcagaagcaa
     2881 gaaaacctac cctaacgaca agaccatcat cagcaccttc aagtggtcct acaccacagg
     2941 caacggcaag agatacagaa gcaccgccag aaccacctac accttcgcca agcccatggc
     3001 cgccaactac ctgaagaacc agcctatgta cgtgttccgc aagaccgagc tgaagcactc
     3061 caagacagaa ctgaacttca aagagtggca gaaagccttc accgacgtga tgggcatgga
     3121 cgagctgtac aagtgagaat tcgcttccac tggattgtac aattaccaac tgatctagaa
     3181 ccggtcatgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     3241 gtgttcgaac tagatgctgt cgaccgatgc gctggtctaa tacccaatct ctcgtcttat
     3301 ccagatgttt tatacgcctt aattaatagg gataacaggg taatatcttg ccctgcacga
     3361 ataccagcga ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact
     3421 tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg
     3481 atctgccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3541 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3601 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3661 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3721 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3781 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3841 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3901 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3961 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4021 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4081 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4141 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4201 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4261 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4321 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4381 gggcttacca tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc
     4441 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4501 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4561 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4621 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4681 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4741 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4801 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4861 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4921 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4981 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5041 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5101 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5161 ttgaagcatt tatcaaggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5221 aaataaacaa ataggggttc cgcgcacgaa ttggccagcg ctgcattacc ctgttatccc
     5281 taaccgg
//
