LOCUS       sequence-396797-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for AWB neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH77 - [AWBp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1882..1898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1882..1898)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1895..1917)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2070..2658)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2150..2169)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2775..2799)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2899..3690,3691..3759))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3522..3541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3760..3864)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3963..3985
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3977..3994
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3978..3994
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4618..4645,1..6))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agttatgata tcaaaaatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta
       61 actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat
      121 acgctaatgc cattgttact ttataatctt tattaaataa gagtactcta taagttcact
      181 ctaggaaaaa attgttacag atctctggaa agaaaatgtt ttgaacactg atctttgaat
      241 cataataatc agaaatcaat tgtgtatcaa ttcactccga aaagtctcgc gactcagaat
      301 ttggttagca gtgtcacact cagtacattt tgtccataaa tcactaaatc aagttatgaa
      361 tagccagcac acgtttttct tccacttcat atccacttcc atatcactat agtctggtga
      421 ttactttaaa aagagaccaa aaatggggcc cgtttcaaag ggagaggccg ttataaaaga
      481 atttatgcgt ttcaaggtcc acatggaggg atccatgaac ggacacgagt tcgagatcga
      541 gggagaggga gagggacgtc catacgaggg aacccaaacc gccaaggtga gttttattat
      601 cgattttcgg gattttctac ttgtaattac cttttgtttt taataagttg cttttttcca
      661 gctcaaggtc accaagggag gaccactccc attctcctgg gacatcctct ccccacaatt
      721 catgtacgga tcccgtgcct tcaccaagca cccagccgac atcccagact actacaagca
      781 atccttccca gagggattca agtgggagcg tgtcatgaac ttcgaggacg gaggagccgt
      841 caccgtcacc caaggtaggt tttcagtttc aatttttgtt gcaactacct caactctatg
      901 ttttcagaca cctccctcga ggacggaacc ctcatctaca aggtcaagct ccgtggaacc
      961 aacttcccac cagacggacc agtcatgcaa aagaagacca tgggatggga ggcctccacc
     1021 gagcgactct acccagagga cggagtcctc aagggagaca tcaaggtaaa tttttcaagc
     1081 ttctaaattc caaaataaac cattttaatt tcttaattcc agatggccct ccggctcaag
     1141 gacggaggac gttacctcgc cgacttcaag accacctaca aggccaagaa gccagtccaa
     1201 atgccaggag cctacaacgt cgaccgtaag ctcgacatca cctcccacaa cgaggactac
     1261 accgtcgtcg agcaatacga gcgttccgag ggacgtcact ccaccggagg aatggacgag
     1321 ctctacaagc ccgggtgatg gtctctgagc tatgcaaaat cctttcaagc attcccttct
     1381 tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat ttgctttttt
     1441 aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa
     1501 tgctatcaaa atgtgattct gatatccagg aaacggagaa tctgtgcggc aatgtcttat
     1561 catttgaaac gcgccgtatc gatgctgaca tcatgcggct cccgtgttag tcgagaagat
     1621 gggtacgggc ctttattcat ccaacactct acggcttctt caagagctag tagggcaccc
     1681 tgtagttgga aagggattat ttcgtatggc gagcccatac cgtctttctt gcggaagctt
     1741 aacacgatag gaagttggat agtttcgaac gatggttcca tacggaacgc tgtctcaagg
     1801 atgagtctga cggagtgtaa ctcgaaggct aggtggaggc tcagtgatga taagtctgcg
     1861 atggtggatg catgtgtcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcag
     1921 agggcacaat cctattccgc gctatccgac aatctccaag acattaggtg gagttcagtt
     1981 cggcgtatgg catatgtcgc tggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2041 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctctattc
     2701 aacaaagccg ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa atcgtcctcg
     2761 tgataccaat tcggagcctg cttttttgta caaacttgtt gataatggca attcaaggat
     2821 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2881 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2941 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3001 gggcttacca tctggcccca gtgctgcaat gataccgcga gagccacgct caccggctcc
     3061 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3121 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3181 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3241 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3301 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3361 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3421 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3481 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3541 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3601 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3661 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3721 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3781 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3841 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccagata cctgaaacaa
     3901 aacccatcgt acggccaagg aagtctccaa taactgtgat ccaccacaag cgccagggtt
     3961 ttcccagtca cgacgttgta aaacgacggc cagtcatgca taatccgcac gcatctggaa
     4021 taaggaagtg ccattccgcc tgacctaagt gcttttcgag atcgccaaac cggagaacga
     4081 tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc
     4141 aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc
     4201 aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt
     4261 tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga
     4321 gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag
     4381 actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag
     4441 cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc
     4501 ataaatcttc attgttgagt ttatcttgtt gatttttgaa taaattatca actctttact
     4561 ttttaatggg ttatgaaata aataaacatt gaaaactgat aaacaacgtt catctctata
     4621 acttcgtata gcatacatta tacga
//
