LOCUS       sequence-396798-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH72 - [AWAp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1922..1938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1922..1938)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1935..1957)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2110..2698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2190..2209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2815..2839)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2939..3730,3731..3799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3562..3581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3800..3904)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4003..4025
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4017..4034
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4018..4034
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4658..4663,1..28))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcgtatagca tacattatac gaagttatga tatcaaaaat gattacgcca agctgtaagt
       61 ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
      121 gaaatcactc acaacgatgg atacgctaat gccattggaa taataaattc aatatatttt
      181 cccaattctg aatttgtgga tacaaacgta ttatttttaa ctgaatacct cgtgaaatca
      241 gatttcagat tttgaaaaca atatatctga ctataagttg cgtatgtaga caaattccag
      301 gaaaataatg gctcaaacga cacgcccctg gcttcaagtt actataaaat aggttaggaa
      361 caaagcacat ataaccatgt attcagttta attaattttt tgttggcggt ggcatgaaat
      421 tctcataatt tcattaagat accttacaga aaaagagacc aaaaatgggg ccctctaagg
      481 gcgaagaatt gttcacggga gtagtcccaa tcttagtcga gcttgacgga ggtgagtttt
      541 attatcgatt ttcgggattt tctacttgta attacctttt gtttttaata agttgctttt
      601 ttccagatgt taatggccac aagttttccg tgtctggaga aggagaaggc gacgctacct
      661 acggtaagct gaccctgaag ttcatttgca ctactggcaa gttaccagta ccatggccga
      721 ccctggtgac aacattttgc tacggggttc aatgcttctc cagataccca ggtaggtttt
      781 cagtttcaat ttttgttgca actacctcaa ctctatgttt tcagatcata tgaaaagaca
      841 cgactttttt aaatccgcca tgccagaggg ttacgttcaa gaacgtacca ttttttttaa
      901 ggacgatggt aactacaaga cacgtgcgga ggttaaattt gaaggcgata ctctcgtgaa
      961 ccgtattgag ctcaaggtaa atttttcaag cttctaaatt ccaaaataaa ccattttaat
     1021 ttcttaattc cagggaatcg atttcaagga agatggaaac attttgggcc acaagcttga
     1081 atataattat aatagtcata atgtctacat tatggccgac aaacaaaaaa acggtattaa
     1141 ggttaacttc aagattcgcc acaatatcga agatggatct gtccaacttg ctgaccacta
     1201 ccaacaaaat actccaattg gagatggacc ggttttgctt ccagataacc actacctttc
     1261 cactcagtct gccctctcta aggatccgaa tgaaaaacgt gaccatatgg ttcttttgga
     1321 gttcgttacc gcagcaggaa ttactcacgg aatggatgaa ctgtacaagc ccgggtgatg
     1381 gtctctgagc tatgcaaaat cctttcaagc attcccttct tctctatcac tcttctttct
     1441 ttttgtcaaa aaattctctc gctaatttat ttgctttttt aatgttatta ttttatgact
     1501 ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa atgtgattct
     1561 gatatccagg aaacggagaa tctgtgcggc aatgtcttat catttgaaac gcgccgtatc
     1621 gatgctgaca tcatgcggct cccgtgttag tcgagaagat gggtacgggc ctttattcat
     1681 ccaacactct acggcttctt caagagctag tagggcaccc tgtagttgga aagggattat
     1741 ttcgtatggc gagcccatac cgtctttctt gcggaagctt aacacgatag gaagttggat
     1801 agtttcgaac gatggttcca tacggaacgc tgtctcaagg atgagtctga cggagtgtaa
     1861 ctcgaaggct aggtggaggc tcagtgatga taagtctgcg atggtggatg catgtgtcat
     1921 ggtcatagct gtttcctgtg tgaaattgtt atccgctcag agggcacaat cctattccgc
     1981 gctatccgac aatctccaag acattaggtg gagttcagtt cggcgtatgg catatgtcgc
     2041 tggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2101 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2161 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2221 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2281 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2341 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2401 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2461 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2521 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2581 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2641 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2701 agatcctttg atcttttcta cggggtctga cgctctattc aacaaagccg ccgtcccgtc
     2761 aagtcagcgt aaatgggtag ggggcttcaa atcgtcctcg tgataccaat tcggagcctg
     2821 cttttttgta caaacttgtt gataatggca attcaaggat cttcacctag atccttttaa
     2881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3061 gtgctgcaat gataccgcga gagccacgct caccggctcc agatttatca gcaataaacc
     3121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3901 cgcgcacatt tccccgaaaa gtgccagata cctgaaacaa aacccatcgt acggccaagg
     3961 aagtctccaa taactgtgat ccaccacaag cgccagggtt ttcccagtca cgacgttgta
     4021 aaacgacggc cagtcatgca taatccgcac gcatctggaa taaggaagtg ccattccgcc
     4081 tgacctaagt gcttttcgag atcgccaaac cggagaacga tcaggagaat gatgagtcgc
     4141 cacaggagtc ggcaagatac gtgcgttata gatttgctcc aaactttttg aaactcaaaa
     4201 cggtcggagc aactgtggaa ttcaaagtag gagacatccc aatccatcat ttccgaatga
     4261 tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt tgactttgaa ttcggattct
     4321 gtattccgaa ttcacgaaac aactgtgaac atatctatga gttccctcaa ctctctcaac
     4381 aactcagtga gtcattattc taaaaagtac aattcaaaag actaatctct ttttcagtgg
     4441 acgacatgat caacaatcca aacgagactc gttctgacag cttctatttc gtcgataaca
     4501 aactcgtcat gcacaacaaa gccgactact catatgatgc ataaatcttc attgttgagt
     4561 ttatcttgtt gatttttgaa taaattatca actctttact ttttaatggg ttatgaaata
     4621 aataaacatt gaaaactgat aaacaacgtt catctctata act
//
