LOCUS       sequence-396800-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for PVP neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH68 - [PVPp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1897..1913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1897..1913)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1910..1932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2085..2673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2165..2184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2790..2814)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2914..3705,3706..3774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3537..3556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3775..3879)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3978..4000
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3992..4009
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3993..4009
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4633..4663,1..3))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tatgatatca aaaatgatta cgccaagctg taagtttaaa catgatctta ctaactaact
       61 attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac gatggatacg
      121 ctaatgcctt gagaaattgc attgtacgat gctgtttatt cgaaatacac aacaccactt
      181 tcaactgttt caatttttga tgttttcagc aagaattctg aacgttctga aaatgtattc
      241 ctgttatttt ttaaacagct gctcatcaac actagcttga atatttaatt actaagcatt
      301 ttcccattaa cttaatcctg acaaaacgaa aggcgaagta gcaattaatc ctacatataa
      361 gaccctaagt taattgaaat gcgtttcttt gattttcatt acttcctcat tttactctta
      421 ctaaaaaaag agaccaaaaa tggggccctc taagggcgaa gaattgttca cgggagtagt
      481 cccaatctta gtcgagcttg acggaggtga gttttattat cgattttcgg gattttctac
      541 ttgtaattac cttttgtttt taataagttg cttttttcca gatgttaatg gccacaagtt
      601 ttccgtgtct ggagaaggag aaggcgacgc tacctacggt aagctgaccc tgaagttcat
      661 ttgcactact ggcaagttac cagtaccatg gccgaccctg gtgacaacat tttgctacgg
      721 ggttcaatgc ttctccagat acccaggtag gttttcagtt tcaatttttg ttgcaactac
      781 ctcaactcta tgttttcaga tcatatgaaa agacacgact tttttaaatc cgccatgcca
      841 gagggttacg ttcaagaacg taccattttt tttaaggacg atggtaacta caagacacgt
      901 gcggaggtta aatttgaagg cgatactctc gtgaaccgta ttgagctcaa ggtaaatttt
      961 tcaagcttct aaattccaaa ataaaccatt ttaatttctt aattccaggg aatcgatttc
     1021 aaggaagatg gaaacatttt gggccacaag cttgaatata attataatag tcataatgtc
     1081 tacattatgg ccgacaaaca aaaaaacggt attaaggtta acttcaagat tcgccacaat
     1141 atcgaagatg gatctgtcca acttgctgac cactaccaac aaaatactcc aattggagat
     1201 ggaccggttt tgcttccaga taaccactac ctttccactc agtctgccct ctctaaggat
     1261 ccgaatgaaa aacgtgacca tatggttctt ttggagttcg ttaccgcagc aggaattact
     1321 cacggaatgg atgaactgta caagcccggg tgatggtctc tgagctatgc aaaatccttt
     1381 caagcattcc cttcttctct atcactcttc tttctttttg tcaaaaaatt ctctcgctaa
     1441 tttatttgct tttttaatgt tattatttta tgacttttta tagtcactga aaagtttgca
     1501 tctgagtgaa gtgaatgcta tcaaaatgtg attctgatat ccaggaaacg gagaatctgt
     1561 gcggcaatgt cttatcattt gaaacgcgcc gtatcgatgc tgacatcatg cggctcccgt
     1621 gttagtcgag aagatgggta cgggccttta ttcatccaac actctacggc ttcttcaaga
     1681 gctagtaggg caccctgtag ttggaaaggg attatttcgt atggcgagcc cataccgtct
     1741 ttcttgcgga agcttaacac gataggaagt tggatagttt cgaacgatgg ttccatacgg
     1801 aacgctgtct caaggatgag tctgacggag tgtaactcga aggctaggtg gaggctcagt
     1861 gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa
     1921 ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct ccaagacatt
     1981 aggtggagtt cagttcggcg tatggcatat gtcgctggaa agaacatgtg agcaaaaggc
     2041 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2101 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2161 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2221 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2281 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2341 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2401 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2461 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2521 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2581 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2641 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2701 tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc
     2761 ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt ttgtacaaac ttgttgataa
     2821 tggcaattca aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2881 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2941 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3001 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagagcc
     3061 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3121 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3181 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3241 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3301 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3361 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3421 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3481 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3541 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3601 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3661 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3721 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3781 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3841 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3901 agatacctga aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc
     3961 acaagcgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc
     4021 cgcacgcatc tggaataagg aagtgccatt ccgcctgacc taagtgcttt tcgagatcgc
     4081 caaaccggag aacgatcagg agaatgatga gtcgccacag gagtcggcaa gatacgtgcg
     4141 ttatagattt gctccaaact ttttgaaact caaaacggtc ggagcaactg tggaattcaa
     4201 agtaggagac atcccaatcc atcatttccg aatgatcgaa cgtcacttct tcaaagatcg
     4261 ccttctgaag tgttttgact ttgaattcgg attctgtatt ccgaattcac gaaacaactg
     4321 tgaacatatc tatgagttcc ctcaactctc tcaacaactc agtgagtcat tattctaaaa
     4381 agtacaattc aaaagactaa tctctttttc agtggacgac atgatcaaca atccaaacga
     4441 gactcgttct gacagcttct atttcgtcga taacaaactc gtcatgcaca acaaagccga
     4501 ctactcatat gatgcataaa tcttcattgt tgagtttatc ttgttgattt ttgaataaat
     4561 tatcaactct ttacttttta atgggttatg aaataaataa acattgaaaa ctgataaaca
     4621 acgttcatct ctataacttc gtatagcata cattatacga agt
//
