LOCUS       sequence-396801-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for ADF neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH75 - [ADFp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1907..1923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1907..1923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1920..1942)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2095..2683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2175..2194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2800..2824)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2924..3715,3716..3784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3547..3566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3785..3889)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3988..4010
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4002..4019
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4003..4019
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4643..4645,1..31))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acttcgtata gcatacatta tacgaagtta tgatatcaaa aatgattacg ccaagctgta
       61 agtttaaaca tgatcttact aactaactat tctcatttaa attttcagag cttaaaaatg
      121 gctgaaatca ctcacaacga tggatacgct aatgcctcac tggtatatct aacatttttt
      181 gttttcaggg tgttatatac attataagat cagtagtata ttaactttaa accactgaat
      241 ttcttctagt tttttgtttc cagctcaagt gctcctgtac gcaaaacatt aggcttcttt
      301 gttcaaaaaa acattttctc accacctcat gacatttttt ttctcatttt tcttattacg
      361 aatgtagcct tattagcata tagcatagag gtacccaaaa ctagtttttt tttgttttga
      421 aatatatatt tgcacctctt ttttcttttt gcaaaaagag accaaaaatg gggcccgttt
      481 caaagggaga ggccgttata aaagaattta tgcgtttcaa ggtccacatg gagggatcca
      541 tgaacggaca cgagttcgag atcgagggag agggagaggg acgtccatac gagggaaccc
      601 aaaccgccaa ggtgagtttt attatcgatt ttcgggattt tctacttgta attacctttt
      661 gtttttaata agttgctttt ttccagctca aggtcaccaa gggaggacca ctcccattct
      721 cctgggacat cctctcccca caattcatgt acggatcccg tgccttcacc aagcacccag
      781 ccgacatccc agactactac aagcaatcct tcccagaggg attcaagtgg gagcgtgtca
      841 tgaacttcga ggacggagga gccgtcaccg tcacccaagg taggttttca gtttcaattt
      901 ttgttgcaac tacctcaact ctatgttttc agacacctcc ctcgaggacg gaaccctcat
      961 ctacaaggtc aagctccgtg gaaccaactt cccaccagac ggaccagtca tgcaaaagaa
     1021 gaccatggga tgggaggcct ccaccgagcg actctaccca gaggacggag tcctcaaggg
     1081 agacatcaag gtaaattttt caagcttcta aattccaaaa taaaccattt taatttctta
     1141 attccagatg gccctccggc tcaaggacgg aggacgttac ctcgccgact tcaagaccac
     1201 ctacaaggcc aagaagccag tccaaatgcc aggagcctac aacgtcgacc gtaagctcga
     1261 catcacctcc cacaacgagg actacaccgt cgtcgagcaa tacgagcgtt ccgagggacg
     1321 tcactccacc ggaggaatgg acgagctcta caagcccggg tgatggtctc tgagctatgc
     1381 aaaatccttt caagcattcc cttcttctct atcactcttc tttctttttg tcaaaaaatt
     1441 ctctcgctaa tttatttgct tttttaatgt tattatttta tgacttttta tagtcactga
     1501 aaagtttgca tctgagtgaa gtgaatgcta tcaaaatgtg attctgatat ccaggaaacg
     1561 gagaatctgt gcggcaatgt cttatcattt gaaacgcgcc gtatcgatgc tgacatcatg
     1621 cggctcccgt gttagtcgag aagatgggta cgggccttta ttcatccaac actctacggc
     1681 ttcttcaaga gctagtaggg caccctgtag ttggaaaggg attatttcgt atggcgagcc
     1741 cataccgtct ttcttgcgga agcttaacac gataggaagt tggatagttt cgaacgatgg
     1801 ttccatacgg aacgctgtct caaggatgag tctgacggag tgtaactcga aggctaggtg
     1861 gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc
     1921 ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct
     1981 ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa agaacatgtg
     2041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2521 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2701 ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg
     2761 ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt ttgtacaaac
     2821 ttgttgataa tggcaattca aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2881 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2941 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3001 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3061 cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3121 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3181 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3241 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3301 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3361 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3421 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3481 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3541 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3601 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3661 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3721 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3781 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3841 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3901 gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc tccaataact
     3961 gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc
     4021 atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc taagtgcttt
     4081 tcgagatcgc caaaccggag aacgatcagg agaatgatga gtcgccacag gagtcggcaa
     4141 gatacgtgcg ttatagattt gctccaaact ttttgaaact caaaacggtc ggagcaactg
     4201 tggaattcaa agtaggagac atcccaatcc atcatttccg aatgatcgaa cgtcacttct
     4261 tcaaagatcg ccttctgaag tgttttgact ttgaattcgg attctgtatt ccgaattcac
     4321 gaaacaactg tgaacatatc tatgagttcc ctcaactctc tcaacaactc agtgagtcat
     4381 tattctaaaa agtacaattc aaaagactaa tctctttttc agtggacgac atgatcaaca
     4441 atccaaacga gactcgttct gacagcttct atttcgtcga taacaaactc gtcatgcaca
     4501 acaaagccga ctactcatat gatgcataaa tcttcattgt tgagtttatc ttgttgattt
     4561 ttgaataaat tatcaactct ttacttttta atgggttatg aaataaataa acattgaaaa
     4621 ctgataaaca acgttcatct ctata
//
