LOCUS       sequence-396802-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for ASK neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH73 - [ASKp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1875..1891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1875..1891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1888..1910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2063..2651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2143..2162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2768..2792)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2892..3683,3684..3752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3515..3534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3753..3857)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3956..3978
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3970..3987
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3971..3987
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4611..4644)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atatcaaaaa tgattacgcc aagctgtaag tttaaacatg atcttactaa ctaactattc
       61 tcatttaaat tttcagagct taaaaatggc tgaaatcact cacaacgatg gatacgctaa
      121 tgccaaatga aatgtcggaa aagaaaatca ttcgaatcaa atgcaccagg aaaaattaag
      181 ttttagaagt gtttaaggta ctttttctat aattatttat tataaaagat atagtcttcc
      241 cttgtcatga catgtggtta attctatgaa agtttgatag aattatgata ttcacataaa
      301 acaaggttgt gatgtctggt gtttcacagt ccgttgactt ttgatgcaat tcttggtaga
      361 catcctccga ctatgtttta gatgtcattt tcaagttttg cagtttctcg aaatattaga
      421 aaaaagagac caaaaatggg gcccgtttca aagggagagg ccgttataaa agaatttatg
      481 cgtttcaagg tccacatgga gggatccatg aacggacacg agttcgagat cgagggagag
      541 ggagagggac gtccatacga gggaacccaa accgccaagg tgagttttat tatcgatttt
      601 cgggattttc tacttgtaat taccttttgt ttttaataag ttgctttttt ccagctcaag
      661 gtcaccaagg gaggaccact cccattctcc tgggacatcc tctccccaca attcatgtac
      721 ggatcccgtg ccttcaccaa gcacccagcc gacatcccag actactacaa gcaatccttc
      781 ccagagggat tcaagtggga gcgtgtcatg aacttcgagg acggaggagc cgtcaccgtc
      841 acccaaggta ggttttcagt ttcaattttt gttgcaacta cctcaactct atgttttcag
      901 acacctccct cgaggacgga accctcatct acaaggtcaa gctccgtgga accaacttcc
      961 caccagacgg accagtcatg caaaagaaga ccatgggatg ggaggcctcc accgagcgac
     1021 tctacccaga ggacggagtc ctcaagggag acatcaaggt aaatttttca agcttctaaa
     1081 ttccaaaata aaccatttta atttcttaat tccagatggc cctccggctc aaggacggag
     1141 gacgttacct cgccgacttc aagaccacct acaaggccaa gaagccagtc caaatgccag
     1201 gagcctacaa cgtcgaccgt aagctcgaca tcacctccca caacgaggac tacaccgtcg
     1261 tcgagcaata cgagcgttcc gagggacgtc actccaccgg aggaatggac gagctctaca
     1321 agcccgggtg atggtctctg agctatgcaa aatcctttca agcattccct tcttctctat
     1381 cactcttctt tctttttgtc aaaaaattct ctcgctaatt tatttgcttt tttaatgtta
     1441 ttattttatg actttttata gtcactgaaa agtttgcatc tgagtgaagt gaatgctatc
     1501 aaaatgtgat tctgatatcc aggaaacgga gaatctgtgc ggcaatgtct tatcatttga
     1561 aacgcgccgt atcgatgctg acatcatgcg gctcccgtgt tagtcgagaa gatgggtacg
     1621 ggcctttatt catccaacac tctacggctt cttcaagagc tagtagggca ccctgtagtt
     1681 ggaaagggat tatttcgtat ggcgagccca taccgtcttt cttgcggaag cttaacacga
     1741 taggaagttg gatagtttcg aacgatggtt ccatacggaa cgctgtctca aggatgagtc
     1801 tgacggagtg taactcgaag gctaggtgga ggctcagtga tgataagtct gcgatggtgg
     1861 atgcatgtgt catggtcata gctgtttcct gtgtgaaatt gttatccgct cagagggcac
     1921 aatcctattc cgcgctatcc gacaatctcc aagacattag gtggagttca gttcggcgta
     1981 tggcatatgt cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2041 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2101 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2161 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2221 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2281 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2341 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2401 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2461 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2521 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2581 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2641 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcta ttcaacaaag
     2701 ccgccgtccc gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc tcgtgatacc
     2761 aattcggagc ctgctttttt gtacaaactt gttgataatg gcaattcaag gatcttcacc
     2821 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2881 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2941 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3001 ccatctggcc ccagtgctgc aatgataccg cgagagccac gctcaccggc tccagattta
     3061 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3121 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3181 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3241 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3301 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3361 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3421 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3481 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3541 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3601 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3661 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3721 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3781 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3841 caaatagggg ttccgcgcac atttccccga aaagtgccag atacctgaaa caaaacccat
     3901 cgtacggcca aggaagtctc caataactgt gatccaccac aagcgccagg gttttcccag
     3961 tcacgacgtt gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa
     4021 gtgccattcc gcctgaccta agtgcttttc gagatcgcca aaccggagaa cgatcaggag
     4081 aatgatgagt cgccacagga gtcggcaaga tacgtgcgtt atagatttgc tccaaacttt
     4141 ttgaaactca aaacggtcgg agcaactgtg gaattcaaag taggagacat cccaatccat
     4201 catttccgaa tgatcgaacg tcacttcttc aaagatcgcc ttctgaagtg ttttgacttt
     4261 gaattcggat tctgtattcc gaattcacga aacaactgtg aacatatcta tgagttccct
     4321 caactctctc aacaactcag tgagtcatta ttctaaaaag tacaattcaa aagactaatc
     4381 tctttttcag tggacgacat gatcaacaat ccaaacgaga ctcgttctga cagcttctat
     4441 ttcgtcgata acaaactcgt catgcacaac aaagccgact actcatatga tgcataaatc
     4501 ttcattgttg agtttatctt gttgattttt gaataaatta tcaactcttt actttttaat
     4561 gggttatgaa ataaataaac attgaaaact gataaacaac gttcatctct ataacttcgt
     4621 atagcataca ttatacgaag ttatg
//
