LOCUS       sequence-396803-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for OLQ neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH69 - [OLQp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(4..37)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1913..1929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1913..1929)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1926..1948)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2101..2689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2181..2200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2806..2830)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2930..3721,3722..3790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3553..3572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3791..3895)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3994..4016
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4008..4025
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4009..4025
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tctataactt cgtatagcat acattatacg aagttatgat atcaaaaatg attacgccaa
       61 gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagagctta
      121 aaaatggctg aaatcactca caacgatgga tacgctaatg cctcattatg ctatttgccg
      181 attttctttc aggaaggttt acggtttgaa taatggcacg aagatacata gatgtattta
      241 taatttaccg atggcttgat tgcattgtaa taaaccgtca ggaaagaaaa ataacatttt
      301 cccagaccct atatgctaca tattactcag tgaaccatag aaatgtcaaa tttccggtta
      361 tgaggcataa gtgagaataa aggtatgtga gcaccgtagt ttcttattta caatttccca
      421 tcgaagaaaa agtaaaatat tctctgaatc taacttgtaa aaagagacca aaaatggggc
      481 ccgtttcaaa gggagaggcc gttataaaag aatttatgcg tttcaaggtc cacatggagg
      541 gatccatgaa cggacacgag ttcgagatcg agggagaggg agagggacgt ccatacgagg
      601 gaacccaaac cgccaaggtg agttttatta tcgattttcg ggattttcta cttgtaatta
      661 ccttttgttt ttaataagtt gcttttttcc agctcaaggt caccaaggga ggaccactcc
      721 cattctcctg ggacatcctc tccccacaat tcatgtacgg atcccgtgcc ttcaccaagc
      781 acccagccga catcccagac tactacaagc aatccttccc agagggattc aagtgggagc
      841 gtgtcatgaa cttcgaggac ggaggagccg tcaccgtcac ccaaggtagg ttttcagttt
      901 caatttttgt tgcaactacc tcaactctat gttttcagac acctccctcg aggacggaac
      961 cctcatctac aaggtcaagc tccgtggaac caacttccca ccagacggac cagtcatgca
     1021 aaagaagacc atgggatggg aggcctccac cgagcgactc tacccagagg acggagtcct
     1081 caagggagac atcaaggtaa atttttcaag cttctaaatt ccaaaataaa ccattttaat
     1141 ttcttaattc cagatggccc tccggctcaa ggacggagga cgttacctcg ccgacttcaa
     1201 gaccacctac aaggccaaga agccagtcca aatgccagga gcctacaacg tcgaccgtaa
     1261 gctcgacatc acctcccaca acgaggacta caccgtcgtc gagcaatacg agcgttccga
     1321 gggacgtcac tccaccggag gaatggacga gctctacaag cccgggtgat ggtctctgag
     1381 ctatgcaaaa tcctttcaag cattcccttc ttctctatca ctcttctttc tttttgtcaa
     1441 aaaattctct cgctaattta tttgcttttt taatgttatt attttatgac tttttatagt
     1501 cactgaaaag tttgcatctg agtgaagtga atgctatcaa aatgtgattc tgatatccag
     1561 gaaacggaga atctgtgcgg caatgtctta tcatttgaaa cgcgccgtat cgatgctgac
     1621 atcatgcggc tcccgtgtta gtcgagaaga tgggtacggg cctttattca tccaacactc
     1681 tacggcttct tcaagagcta gtagggcacc ctgtagttgg aaagggatta tttcgtatgg
     1741 cgagcccata ccgtctttct tgcggaagct taacacgata ggaagttgga tagtttcgaa
     1801 cgatggttcc atacggaacg ctgtctcaag gatgagtctg acggagtgta actcgaaggc
     1861 taggtggagg ctcagtgatg ataagtctgc gatggtggat gcatgtgtca tggtcatagc
     1921 tgtttcctgt gtgaaattgt tatccgctca gagggcacaa tcctattccg cgctatccga
     1981 caatctccaa gacattaggt ggagttcagt tcggcgtatg gcatatgtcg ctggaaagaa
     2041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2701 gatcttttct acggggtctg acgctctatt caacaaagcc gccgtcccgt caagtcagcg
     2761 taaatgggta gggggcttca aatcgtcctc gtgataccaa ttcggagcct gcttttttgt
     2821 acaaacttgt tgataatggc aattcaagga tcttcaccta gatcctttta aattaaaaat
     2881 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2941 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3001 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3061 tgataccgcg agagccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3121 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3181 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3241 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3301 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3361 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3421 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3481 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3541 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3601 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3661 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3721 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3781 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3841 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3901 ttccccgaaa agtgccagat acctgaaaca aaacccatcg tacggccaag gaagtctcca
     3961 ataactgtga tccaccacaa gcgccagggt tttcccagtc acgacgttgt aaaacgacgg
     4021 ccagtcatgc ataatccgca cgcatctgga ataaggaagt gccattccgc ctgacctaag
     4081 tgcttttcga gatcgccaaa ccggagaacg atcaggagaa tgatgagtcg ccacaggagt
     4141 cggcaagata cgtgcgttat agatttgctc caaacttttt gaaactcaaa acggtcggag
     4201 caactgtgga attcaaagta ggagacatcc caatccatca tttccgaatg atcgaacgtc
     4261 acttcttcaa agatcgcctt ctgaagtgtt ttgactttga attcggattc tgtattccga
     4321 attcacgaaa caactgtgaa catatctatg agttccctca actctctcaa caactcagtg
     4381 agtcattatt ctaaaaagta caattcaaaa gactaatctc tttttcagtg gacgacatga
     4441 tcaacaatcc aaacgagact cgttctgaca gcttctattt cgtcgataac aaactcgtca
     4501 tgcacaacaa agccgactac tcatatgatg cataaatctt cattgttgag tttatcttgt
     4561 tgatttttga ataaattatc aactctttac tttttaatgg gttatgaaat aaataaacat
     4621 tgaaaactga taaacaacgt tcatc
//
