LOCUS       sequence-396804-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for OLQ neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH70 - [OLQp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1876..1892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1876..1892)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1889..1911)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2064..2652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2144..2163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2769..2793)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2893..3684,3685..3753))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3516..3535
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3754..3858)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3957..3979
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3971..3988
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3972..3988
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4612..4645)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga
       61 gcttaaaaat ggctgaaatc actcacaacg atggatacgc taatgcctca ttatgctatt
      121 tgccgatttt ctttcaggaa ggtttacggt ttgaataatg gcacgaagat acatagatgt
      181 atttataatt taccgatggc ttgattgcat tgtaataaac cgtcaggaaa gaaaaataac
      241 attttcccag accctatatg ctacatatta ctcagtgaac catagaaatg tcaaatttcc
      301 ggttatgagg cataagtgag aataaaggta tgtgagcacc gtagtttctt atttacaatt
      361 tcccatcgaa gaaaaagtaa aatattctct gaatctaact tgtaaaaaga gaccaaaaat
      421 ggggccctct aagggcgaag aattgttcac gggagtagtc ccaatcttag tcgagcttga
      481 cggaggtgag ttttattatc gattttcggg attttctact tgtaattacc ttttgttttt
      541 aataagttgc ttttttccag atgttaatgg ccacaagttt tccgtgtctg gagaaggaga
      601 aggcgacgct acctacggta agctgaccct gaagttcatt tgcactactg gcaagttacc
      661 agtaccatgg ccgaccctgg tgacaacatt ttgctacggg gttcaatgct tctccagata
      721 cccaggtagg ttttcagttt caatttttgt tgcaactacc tcaactctat gttttcagat
      781 catatgaaaa gacacgactt ttttaaatcc gccatgccag agggttacgt tcaagaacgt
      841 accatttttt ttaaggacga tggtaactac aagacacgtg cggaggttaa atttgaaggc
      901 gatactctcg tgaaccgtat tgagctcaag gtaaattttt caagcttcta aattccaaaa
      961 taaaccattt taatttctta attccaggga atcgatttca aggaagatgg aaacattttg
     1021 ggccacaagc ttgaatataa ttataatagt cataatgtct acattatggc cgacaaacaa
     1081 aaaaacggta ttaaggttaa cttcaagatt cgccacaata tcgaagatgg atctgtccaa
     1141 cttgctgacc actaccaaca aaatactcca attggagatg gaccggtttt gcttccagat
     1201 aaccactacc tttccactca gtctgccctc tctaaggatc cgaatgaaaa acgtgaccat
     1261 atggttcttt tggagttcgt taccgcagca ggaattactc acggaatgga tgaactgtac
     1321 aagcccgggt gatggtctct gagctatgca aaatcctttc aagcattccc ttcttctcta
     1381 tcactcttct ttctttttgt caaaaaattc tctcgctaat ttatttgctt ttttaatgtt
     1441 attattttat gactttttat agtcactgaa aagtttgcat ctgagtgaag tgaatgctat
     1501 caaaatgtga ttctgatatc caggaaacgg agaatctgtg cggcaatgtc ttatcatttg
     1561 aaacgcgccg tatcgatgct gacatcatgc ggctcccgtg ttagtcgaga agatgggtac
     1621 gggcctttat tcatccaaca ctctacggct tcttcaagag ctagtagggc accctgtagt
     1681 tggaaaggga ttatttcgta tggcgagccc ataccgtctt tcttgcggaa gcttaacacg
     1741 ataggaagtt ggatagtttc gaacgatggt tccatacgga acgctgtctc aaggatgagt
     1801 ctgacggagt gtaactcgaa ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
     1861 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
     1921 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt
     1981 atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2041 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2101 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2161 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2221 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2281 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2401 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2461 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2521 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2581 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2641 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa
     2701 gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc ctcgtgatac
     2761 caattcggag cctgcttttt tgtacaaact tgttgataat ggcaattcaa ggatcttcac
     2821 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2881 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2941 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3001 accatctggc cccagtgctg caatgatacc gcgagagcca cgctcaccgg ctccagattt
     3061 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3121 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3181 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3241 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3301 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3361 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3421 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3481 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3541 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3601 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3661 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3721 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3781 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3841 acaaataggg gttccgcgca catttccccg aaaagtgcca gatacctgaa acaaaaccca
     3901 tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca
     3961 gtcacgacgt tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga
     4021 agtgccattc cgcctgacct aagtgctttt cgagatcgcc aaaccggaga acgatcagga
     4081 gaatgatgag tcgccacagg agtcggcaag atacgtgcgt tatagatttg ctccaaactt
     4141 tttgaaactc aaaacggtcg gagcaactgt ggaattcaaa gtaggagaca tcccaatcca
     4201 tcatttccga atgatcgaac gtcacttctt caaagatcgc cttctgaagt gttttgactt
     4261 tgaattcgga ttctgtattc cgaattcacg aaacaactgt gaacatatct atgagttccc
     4321 tcaactctct caacaactca gtgagtcatt attctaaaaa gtacaattca aaagactaat
     4381 ctctttttca gtggacgaca tgatcaacaa tccaaacgag actcgttctg acagcttcta
     4441 tttcgtcgat aacaaactcg tcatgcacaa caaagccgac tactcatatg atgcataaat
     4501 cttcattgtt gagtttatct tgttgatttt tgaataaatt atcaactctt tactttttaa
     4561 tgggttatga aataaataaa cattgaaaac tgataaacaa cgttcatctc tataacttcg
     4621 tatagcatac attatacgaa gttatgatat caaaaatgat tac
//
