LOCUS       sequence-396806-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for ASK neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH74 - [ASKp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1906..1922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1906..1922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1919..1941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2094..2682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2174..2193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2799..2823)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2923..3714,3715..3783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3546..3565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3784..3888)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3987..4009
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4001..4018
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4002..4018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4642..4663,1..12))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atacgaagtt atgatatcaa aaatgattac gccaagctgt aagtttaaac atgatcttac
       61 taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg
      121 atggatacgc taatgccaaa tgaaatgtcg gaaaagaaaa tcattcgaat caaatgcacc
      181 aggaaaaatt aagttttaga agtgtttaag gtactttttc tataattatt tattataaaa
      241 gatatagtct tcccttgtca tgacatgtgg ttaattctat gaaagtttga tagaattatg
      301 atattcacat aaaacaaggt tgtgatgtct ggtgtttcac agtccgttga cttttgatgc
      361 aattcttggt agacatcctc cgactatgtt ttagatgtca ttttcaagtt ttgcagtttc
      421 tcgaaatatt agaaaaaaga gaccaaaaat ggggccctct aagggcgaag aattgttcac
      481 gggagtagtc ccaatcttag tcgagcttga cggaggtgag ttttattatc gattttcggg
      541 attttctact tgtaattacc ttttgttttt aataagttgc ttttttccag atgttaatgg
      601 ccacaagttt tccgtgtctg gagaaggaga aggcgacgct acctacggta agctgaccct
      661 gaagttcatt tgcactactg gcaagttacc agtaccatgg ccgaccctgg tgacaacatt
      721 ttgctacggg gttcaatgct tctccagata cccaggtagg ttttcagttt caatttttgt
      781 tgcaactacc tcaactctat gttttcagat catatgaaaa gacacgactt ttttaaatcc
      841 gccatgccag agggttacgt tcaagaacgt accatttttt ttaaggacga tggtaactac
      901 aagacacgtg cggaggttaa atttgaaggc gatactctcg tgaaccgtat tgagctcaag
      961 gtaaattttt caagcttcta aattccaaaa taaaccattt taatttctta attccaggga
     1021 atcgatttca aggaagatgg aaacattttg ggccacaagc ttgaatataa ttataatagt
     1081 cataatgtct acattatggc cgacaaacaa aaaaacggta ttaaggttaa cttcaagatt
     1141 cgccacaata tcgaagatgg atctgtccaa cttgctgacc actaccaaca aaatactcca
     1201 attggagatg gaccggtttt gcttccagat aaccactacc tttccactca gtctgccctc
     1261 tctaaggatc cgaatgaaaa acgtgaccat atggttcttt tggagttcgt taccgcagca
     1321 ggaattactc acggaatgga tgaactgtac aagcccgggt gatggtctct gagctatgca
     1381 aaatcctttc aagcattccc ttcttctcta tcactcttct ttctttttgt caaaaaattc
     1441 tctcgctaat ttatttgctt ttttaatgtt attattttat gactttttat agtcactgaa
     1501 aagtttgcat ctgagtgaag tgaatgctat caaaatgtga ttctgatatc caggaaacgg
     1561 agaatctgtg cggcaatgtc ttatcatttg aaacgcgccg tatcgatgct gacatcatgc
     1621 ggctcccgtg ttagtcgaga agatgggtac gggcctttat tcatccaaca ctctacggct
     1681 tcttcaagag ctagtagggc accctgtagt tggaaaggga ttatttcgta tggcgagccc
     1741 ataccgtctt tcttgcggaa gcttaacacg ataggaagtt ggatagtttc gaacgatggt
     1801 tccatacgga acgctgtctc aaggatgagt ctgacggagt gtaactcgaa ggctaggtgg
     1861 aggctcagtg atgataagtc tgcgatggtg gatgcatgtg tcatggtcat agctgtttcc
     1921 tgtgtgaaat tgttatccgc tcagagggca caatcctatt ccgcgctatc cgacaatctc
     1981 caagacatta ggtggagttc agttcggcgt atggcatatg tcgctggaaa gaacatgtga
     2041 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2101 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2161 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2221 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2281 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2341 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2401 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2461 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2521 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2581 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2641 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2701 tctacggggt ctgacgctct attcaacaaa gccgccgtcc cgtcaagtca gcgtaaatgg
     2761 gtagggggct tcaaatcgtc ctcgtgatac caattcggag cctgcttttt tgtacaaact
     2821 tgttgataat ggcaattcaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2881 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2941 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3001 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3061 gcgagagcca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3121 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3181 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3241 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3301 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3361 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3421 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3481 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3541 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3601 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3661 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3721 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3781 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3841 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3901 aaaagtgcca gatacctgaa acaaaaccca tcgtacggcc aaggaagtct ccaataactg
     3961 tgatccacca caagcgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtca
     4021 tgcataatcc gcacgcatct ggaataagga agtgccattc cgcctgacct aagtgctttt
     4081 cgagatcgcc aaaccggaga acgatcagga gaatgatgag tcgccacagg agtcggcaag
     4141 atacgtgcgt tatagatttg ctccaaactt tttgaaactc aaaacggtcg gagcaactgt
     4201 ggaattcaaa gtaggagaca tcccaatcca tcatttccga atgatcgaac gtcacttctt
     4261 caaagatcgc cttctgaagt gttttgactt tgaattcgga ttctgtattc cgaattcacg
     4321 aaacaactgt gaacatatct atgagttccc tcaactctct caacaactca gtgagtcatt
     4381 attctaaaaa gtacaattca aaagactaat ctctttttca gtggacgaca tgatcaacaa
     4441 tccaaacgag actcgttctg acagcttcta tttcgtcgat aacaaactcg tcatgcacaa
     4501 caaagccgac tactcatatg atgcataaat cttcattgtt gagtttatct tgttgatttt
     4561 tgaataaatt atcaactctt tactttttaa tgggttatga aataaataaa cattgaaaac
     4621 tgataaacaa cgttcatctc tataacttcg tatagcatac att
//
