LOCUS       sequence-396811-        7098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDV06 with iRFP720-targeted sensor sequence inserted. Expresses 
            TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon 
            detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Gayet RV, Ilia K, Razavi S, Tippens ND, Lalwani MA, Zhang K, Chen 
            JX, Chen JC, Vargas-Asencio J, Collins JJ
  TITLE     Autocatalytic base editing for RNA-responsive translational control.
  JOURNAL   Nat Commun. 2023 Mar 11;14(1):1339. doi: 10.1038/s41467-023-36851-z.
  PUBMED    36906659
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-11"; volume: "14(1):1339. doi"; pages: " 
            10.1038/s41467-023-36851-z"
COMMENT     Sequence Label: pDV06.2c_DART-VADAR_iRFP720-sensor
FEATURES             Location/Qualifiers
     source          1..7098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        348..559
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          560..1498
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1455..1475
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2246..2302
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_RNA        2353..2371
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        2422..2440
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             2483..2536
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2798..2807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3260..3319
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             4925..4951
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     polyA_signal    5006..5054
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(5331..5919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5411..5430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6090..6881,6882..6950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6713..6732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6951..7055)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcgttttta tgcttgtagt attgtataat gtttttaaga tccggagaga gcgagattcc
       61 gtctcaaaga aaaaaaaagt aatgaaatga ataaaatgag tcctagagcc agtaaatgtc
      121 gtaaatgtct cagctagtca ggtagtaaaa ggtctcaact aggcagtggc agagcaggat
      181 tcaaattcag ggctgttgtg atgcctccgc agactctgag cgccacctgg tggtaatttg
      241 tctgtgcctc ttctgacgtg gaagaacagc aactaacaca ctaacacggc atttactatg
      301 ggccagccat tgttctaata ctttaattgg ctccggtgcc cgtcagtggg cagagcgcac
      361 atcgcccaca gtccccgaga agttgtgggg aggggtcggc aattgaaccg gtgcctagag
      421 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
      481 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
      541 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
      601 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
      661 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
      721 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
      781 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
      841 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
      901 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
      961 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     1021 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     1081 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     1141 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     1201 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     1261 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     1321 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     1381 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     1441 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     1501 ggatcccaag ggcagataag tttgtacaaa aaagagaggt gccaccatga gcgagctgat
     1561 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     1621 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     1681 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     1741 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     1801 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     1861 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     1921 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     1981 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     2041 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     2101 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     2161 caacaacgag acatacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     2221 tagcaaactg gggcacggta gcggcgcaac aaacttctct cttctgaaac aggcaggaga
     2281 tgtggaagaa aatccaggcc caggatccgg tttaattaag ggcctgcagg gtggacgatc
     2341 gtcatgtcgg cgacatgagg atcacccatg tcgagcagca gtccatgcgg ttggatagca
     2401 ccggggatat ggatcggctc aacatgagga tcacccatgt aggtcaagag gtcaggcttc
     2461 tactcgaggg gctagccagg gagagggcag aggaagtctg cttacatgcg gtgatgtcga
     2521 agagaatcct ggcccaggta ccatggtgtc caagggcgaa gaggacaaca tggccagcct
     2581 gcctgccacc cacgagctgc acatcttcgg cagcatcaac ggcgtggact tcgacatggt
     2641 gggacagggc accggcaacc ccaacgacgg atacgaggaa ctgaacctga agtccacaaa
     2701 gggcgacctg cagttcagcc cctggatcct ggtgccccac atcggctacg gcttccacca
     2761 gtacctgccc taccctgacg gcatgagccc tttccaggcc gccatggtgg atggctctgg
     2821 ctaccaggtg cacagaacca tgcagttcga ggacggcgcc agcctgaccg tgaactacag
     2881 atacacctac gagggcagcc acatcaaggg cgaggcccaa gtgaagggca caggcttccc
     2941 tgctgacggc cccgtgatga ccaactctct gacagccgcc gactggtgca gaagcaagaa
     3001 aacctaccct aacgacaaga ccatcatcag caccttcaag tggtcctaca ccacaggcaa
     3061 cggcaagaga tacagaagca ccgccagaac cacctacacc ttcgccaagc ccatggccgc
     3121 caactacctg aagaaccagc ctatgtacgt gttccgcaag accgagctga agcactccaa
     3181 gacagaactg aacttcaaag agtggcagaa agccttcacc gacgtgatgg gcatggacga
     3241 gctgtacaag ggctctgggc agtgtactaa ttatgctctc ttgaaattgg ctggagatgt
     3301 tgagagcaac ccaggtcccg gctctggaat ggcgtccaat ttcactcagt ttgtgctggt
     3361 tgacaacggc gggaccgggg acgttacggt agccccctca aactttgcca acggtatagc
     3421 ggagtggata agcagcaatt ctaggagtca agcatacaaa gttacatgca gcgtgcgcca
     3481 atctagcgct cagaatcgca agtacaccat taaagtagag gtccccaagg gagcctggag
     3541 aagctatctt aacatggagt tgaccatacc aatcttcgct accaactctg actgtgaact
     3601 cattgtgaaa gccatgcaag gtctgctcaa ggatggtaac ccaattccgt ccgctatcgc
     3661 tgccaactct gggatttacg ggggcagtgg gagcggtgca ggatctggta gtccagctgg
     3721 gggaggagca ccgggtagcg gtggggggtc tcagctgcac ctgccccagg ttctcgcaga
     3781 cgccgtatcc cgccttgtac tgggcaagtt tggtgatctt actgacaatt tttcatctcc
     3841 tcatgcgagg cggaaagtac tcgcaggcgt cgtcatgacg accggaactg acgtgaaaga
     3901 cgccaaagtc atctctgtct ccacgggcac aaagtgcata aacggggagt acatgagcga
     3961 ccgggggctg gcactgaatg attgtcacgc tgaaataata tctaggcgat ctctgcttag
     4021 atttctctac actcaactcg aattgtacct taacaacaaa gatgaccaga aacgcagtat
     4081 atttcagaaa tcagaacgcg gcggatttcg acttaaggaa aacgttcagt tccacttgta
     4141 tatcagcaca tccccttgcg gtgacgcccg aatcttttcc ccgcacgagc cgatattgga
     4201 ggagcccgcg gacagacatc ctaataggaa ggctagaggc caacttcgga cgaagattga
     4261 aagtggccag ggtactatcc cggtgcggtc caacgctagt attcaaacgt gggacggagt
     4321 ccttcaaggt gaacggctgt tgacaatgag ctgctcagac aaaatcgcgc gctggaatgt
     4381 agtgggaatc caaggcagcc tcttgagcat attcgtagaa cccatatatt tctcatccat
     4441 tattttgggc tctctgtatc atggtgacca tctgtcaagg gctatgtacc aacgaatttc
     4501 taatatcgag gatcttcctc cactctatac actcaataag cctctcttgt ccgggatatc
     4561 aaacgctgag gcccgccagc cagggaaagc tcctaacttc agtgttaact ggaccgttgg
     4621 tgattctgcg atagaggtca tcaacgccac gacaggtaag gatgagctcg gtagagcctc
     4681 acgcctgtgt aaacacgcgt tgtattgtag atggatgaga gtacatggga aggtcccatc
     4741 tcacttgctc cgaagcaaga tcactaagcc taatgtgtat catgagtcaa aactcgcggc
     4801 taaagaatac caggcagcca aagctcgact ttttacagct tttattaagg cagggctcgg
     4861 ggcatgggtc gagaagccga ccgagcagga ccaattctct ctgacgggga gcggatccgg
     4921 ctctctgcca ccattggaaa ggcttacgct gtaacttcca ctggattgta caattaccaa
     4981 ctgatctaga accggtcatg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     5041 tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg cgctggtcta atacccaatc
     5101 tctcgtctta tccagatgtt ttatacgcct taattaatag ggataacagg gtaatatctt
     5161 gccctgcacg aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga
     5221 gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg
     5281 ccacatctag gatctgccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5761 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6001 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6061 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6121 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6181 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     6241 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6301 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6361 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6421 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6481 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6541 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6601 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6661 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6721 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6781 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6841 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6901 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6961 tttcaatatt attgaagcat ttatcaaggt tattgtctca tgagcggata catatttgaa
     7021 tgtatttaga aaaataaaca aataggggtt ccgcgcacga attggccagc gctgcattac
     7081 cctgttatcc ctaaccgg
//
