LOCUS       sequence-396812-        7073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Gayet RV, Ilia K, Razavi S, Tippens ND, Lalwani MA, Zhang K, Chen 
            JX, Chen JC, Vargas-Asencio J, Collins JJ
  TITLE     Autocatalytic base editing for RNA-responsive translational control.
  JOURNAL   Nat Commun. 2023 Mar 11;14(1):1339. doi: 10.1038/s41467-023-36851-z.
  PUBMED    36906659
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-11"; volume: "14(1):1339. doi"; pages: " 
            10.1038/s41467-023-36851-z"
COMMENT     Sequence Label: pDV24_AAV2_DART-VADAR_iRFP720-sensor
FEATURES             Location/Qualifiers
     source          1..7073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        204..507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        508..711
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     662..682
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1455..1511
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_RNA        1562..1580
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1631..1649
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             1692..1745
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2007..2016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2469..2528
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             4134..4160
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     polyA_signal    4215..4263
                     /note="synthetic polyadenylation signal"
     repeat_region   4336..4476
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4336..4465
                     /label=AAV2 ITR
     rep_origin      4551..5006
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4638..4657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4848..4869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5023..5042)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5142..5164
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5202..5220)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5288..5392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5393..5461,5462..6253)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5611..5630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6424..7012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6913..6932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gactagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      721 aaccgtcaga tccgctagcg ctaccggtcg ccaccatgag cgagctgatt aaggagaaca
      781 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
      841 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
      901 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
      961 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     1021 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     1081 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     1141 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     1201 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     1261 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     1321 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     1381 catacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     1441 ggcacggtag cggcgcaaca aacttctctc ttctgaaaca ggcaggagat gtggaagaaa
     1501 atccaggccc aggatccggt ttaattaagg gcctgcaggg tggacgatcg tcatgtcggc
     1561 gacatgagga tcacccatgt cgagcagcag tccatgcggt tggatagcac cggggatatg
     1621 gatcggctca acatgaggat cacccatgta ggtcaagagg tcaggcttct actcgagggg
     1681 ctagccaggg agagggcaga ggaagtctgc ttacatgcgg tgatgtcgaa gagaatcctg
     1741 gcccaggtac catggtgtcc aagggcgaag aggacaacat ggccagcctg cctgccaccc
     1801 acgagctgca catcttcggc agcatcaacg gcgtggactt cgacatggtg ggacagggca
     1861 ccggcaaccc caacgacgga tacgaggaac tgaacctgaa gtccacaaag ggcgacctgc
     1921 agttcagccc ctggatcctg gtgccccaca tcggctacgg cttccaccag tacctgccct
     1981 accctgacgg catgagccct ttccaggccg ccatggtgga tggctctggc taccaggtgc
     2041 acagaaccat gcagttcgag gacggcgcca gcctgaccgt gaactacaga tacacctacg
     2101 agggcagcca catcaagggc gaggcccaag tgaagggcac aggcttccct gctgacggcc
     2161 ccgtgatgac caactctctg acagccgccg actggtgcag aagcaagaaa acctacccta
     2221 acgacaagac catcatcagc accttcaagt ggtcctacac cacaggcaac ggcaagagat
     2281 acagaagcac cgccagaacc acctacacct tcgccaagcc catggccgcc aactacctga
     2341 agaaccagcc tatgtacgtg ttccgcaaga ccgagctgaa gcactccaag acagaactga
     2401 acttcaaaga gtggcagaaa gccttcaccg acgtgatggg catggacgag ctgtacaagg
     2461 gctctgggca gtgtactaat tatgctctct tgaaattggc tggagatgtt gagagcaacc
     2521 caggtcccgg ctctggaatg gcgtccaatt tcactcagtt tgtgctggtt gacaacggcg
     2581 ggaccgggga cgttacggta gccccctcaa actttgccaa cggtatagcg gagtggataa
     2641 gcagcaattc taggagtcaa gcatacaaag ttacatgcag cgtgcgccaa tctagcgctc
     2701 agaatcgcaa gtacaccatt aaagtagagg tccccaaggg agcctggaga agctatctta
     2761 acatggagtt gaccatacca atcttcgcta ccaactctga ctgtgaactc attgtgaaag
     2821 ccatgcaagg tctgctcaag gatggtaacc caattccgtc cgctatcgct gccaactctg
     2881 ggatttacgg gggcagtggg agcggtgcag gatctggtag tccagctggg ggaggagcac
     2941 cgggtagcgg tggggggtct cagctgcacc tgccccaggt tctcgcagac gccgtatccc
     3001 gccttgtact gggcaagttt ggtgatctta ctgacaattt ttcatctcct catgcgaggc
     3061 ggaaagtact cgcaggcgtc gtcatgacga ccggaactga cgtgaaagac gccaaagtca
     3121 tctctgtctc cacgggcaca aagtgcataa acggggagta catgagcgac cgggggctgg
     3181 cactgaatga ttgtcacgct gaaataatat ctaggcgatc tctgcttaga tttctctaca
     3241 ctcaactcga attgtacctt aacaacaaag atgaccagaa acgcagtata tttcagaaat
     3301 cagaacgcgg cggatttcga cttaaggaaa acgttcagtt ccacttgtat atcagcacat
     3361 ccccttgcgg tgacgcccga atcttttccc cgcacgagcc gatattggag gagcccgcgg
     3421 acagacatcc taataggaag gctagaggcc aacttcggac gaagattgaa agtggccagg
     3481 gtactatccc ggtgcggtcc aacgctagta ttcaaacgtg ggacggagtc cttcaaggtg
     3541 aacggctgtt gacaatgagc tgctcagaca aaatcgcgcg ctggaatgta gtgggaatcc
     3601 aaggcagcct cttgagcata ttcgtagaac ccatatattt ctcatccatt attttgggct
     3661 ctctgtatca tggtgaccat ctgtcaaggg ctatgtacca acgaatttct aatatcgagg
     3721 atcttcctcc actctataca ctcaataagc ctctcttgtc cgggatatca aacgctgagg
     3781 cccgccagcc agggaaagct cctaacttca gtgttaactg gaccgttggt gattctgcga
     3841 tagaggtcat caacgccacg acaggtaagg atgagctcgg tagagcctca cgcctgtgta
     3901 aacacgcgtt gtattgtaga tggatgagag tacatgggaa ggtcccatct cacttgctcc
     3961 gaagcaagat cactaagcct aatgtgtatc atgagtcaaa actcgcggct aaagaatacc
     4021 aggcagccaa agctcgactt tttacagctt ttattaaggc agggctcggg gcatgggtcg
     4081 agaagccgac cgagcaggac caattctctc tgacggggag cggatccggc tctctgccac
     4141 cattggaaag gcttacgctg taacttccac tggattgtac aattaccaac tgatctagaa
     4201 ccggtcatgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     4261 gtgttcgaac tagatgctgt cgaccgatgc gctggtctaa tacccaatct ctcgtcttat
     4321 ccagatggcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     4381 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     4441 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     4501 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     4561 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4621 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4681 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4741 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     4801 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4861 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     4921 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4981 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     5041 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5101 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5161 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     5221 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5281 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5341 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5401 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5461 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5521 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5581 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5641 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5701 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5761 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5821 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5881 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5941 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6001 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6061 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6121 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6181 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6241 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6301 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6361 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6541 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
