LOCUS       sequence-396814-        9659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human INSR receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-INSR-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..39)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        211..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     745..765
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     839..858
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        839..857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5133..5174
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5184..5201
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5224..5241)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5230..5454
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5500..5928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5587..5606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5797..5818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5937..5957)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5942..6271
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6122..6257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6338..7132
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6392..6411)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7002..7021
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7308..7429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7345..7364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7399..7418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7478..7494)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7478..7494)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7491..7513)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7502..7518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7526..7532,7533..7550,7551..7556))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7571..7592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7709..7726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7880..8468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7960..7979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8639..9430,9431..9499))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9262..9281
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9500..9604)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
       61 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
      121 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
      181 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta
      841 atacgactca ctatagggag acccaagctg gctagttaag cttggtaccg agctcggatc
      901 cagtaccctt caccatgggc accgggggcc ggcggggggc ggcggccgcg ccgctgctgg
      961 tggcggtggc cgcgctgcta ctgggcgccg cgggccacct gtaccccgga gaggtgtgtc
     1021 ccggcatgga tatccggaac aacctcacta ggttgcatga gctggagaat tgctctgtca
     1081 tcgaaggaca cttgcagata ctcttgatgt tcaaaacgag gcccgaagat ttccgagacc
     1141 tcagtttccc caaactcatc atgatcactg attacttgct gctcttccgg gtctatgggc
     1201 tcgagagcct gaaggacctg ttccccaacc tcacggtcat ccggggatca cgactgttct
     1261 ttaactacgc gctggtcatc ttcgagatgg ttcacctcaa ggaactcggc ctctacaacc
     1321 tgatgaacat cacccggggt tctgtccgca tcgagaagaa caatgagctc tgttacttgg
     1381 ccactatcga ctggtcccgt atcctggatt ccgtggagga taattacatc gtgttgaaca
     1441 aagatgacaa cgaggagtgt ggagacatct gtccgggtac cgcgaagggc aagaccaact
     1501 gccccgccac cgtcatcaac gggcagtttg tcgaacgatg ttggactcat agtcactgcc
     1561 agaaagtttg cccgaccatc tgtaagtcac acggctgcac cgccgaaggc ctctgttgcc
     1621 acagcgagtg cctgggcaac tgttctcagc ccgacgaccc caccaagtgc gtggcctgcc
     1681 gcaacttcta cctggacggc aggtgtgtgg agacctgccc gcccccgtac taccacttcc
     1741 aggactggcg ctgtgtgaac ttcagcttct gccaggacct gcaccacaaa tgcaagaact
     1801 cgcggaggca gggctgccac cagtacgtca ttcacaacaa caagtgcatc cctgagtgtc
     1861 cctccgggta cacgatgaat tccagcaact tgctgtgcac cccatgcctg ggtccctgtc
     1921 ccaaggtgtg ccacctccta gaaggcgaga agaccatcga ctcggtgacg tctgcccagg
     1981 agctccgagg atgcaccgtc atcaacggga gtctgatcat caacattcga ggaggcaaca
     2041 atctggcagc tgagctagaa gccaacctcg gcctcattga agaaatttca gggtatctaa
     2101 aaatccgccg atcctacgct ctggtgtcac tttccttctt ccggaagtta cgtctgattc
     2161 gaggagagac cttggaaatt gggaactact ccttctatgc cttggacaac cagaacctaa
     2221 ggcagctctg ggactggagc aaacacaacc tcaccatcac tcaggggaaa ctcttcttcc
     2281 actataaccc caaactctgc ttgtcagaaa tccacaagat ggaagaagtt tcaggaacca
     2341 aggggcgcca ggagagaaac gacattgccc tgaagaccaa tggggaccag gcatcctgtg
     2401 aaaatgagtt acttaaattt tcttacattc ggacatcttt tgacaagatc ttgctgagat
     2461 gggagccgta ctggcccccc gacttccgag acctcttggg gttcatgctg ttctacaaag
     2521 aggcccctta tcagaatgtg acggagttcg acgggcagga tgcgtgtggt tccaacagtt
     2581 ggacggtggt agacattgac ccacccctga ggtccaacga ccccaaatca cagaaccacc
     2641 cagggtggct gatgcggggt ctcaagccct ggacccagta tgccatcttt gtgaagaccc
     2701 tggtcacctt ttcggatgaa cgccggacct atggggccaa gagtgacatc atttatgtcc
     2761 agacagatgc caccaacccc tctgtgcccc tggatccaat ctcagtgtct aactcatcat
     2821 cccagattat tctgaagtgg aaaccaccct ccgaccccaa tggcaacatc acccactacc
     2881 tggttttctg ggagaggcag gcggaagaca gtgagctgtt cgagctggat tattgcctca
     2941 aagggctgaa gctgccctcg aggacctggt ctccaccatt cgagtctgaa gattctcaga
     3001 agcacaacca gagtgagtat gaggattcgg ccggcgaatg ctgctcctgt ccaaagacag
     3061 actctcagat cctgaaggag ctggaggagt cctcgtttag gaagacgttt gaggattacc
     3121 tgcacaacgt ggttttcgtc cccagaaaaa cctcttcagg cactggtgcc gaggacccta
     3181 ggccatctcg gaaacgcagg tcccttggcg atgttgggaa tgtgacggtg gccgtgccca
     3241 cggtggcagc tttccccaac acttcctcga ccagcgtgcc cacgagtccg gaggagcaca
     3301 ggccttttga gaaggtggtg aacaaggagt cgctggtcat ctccggcttg cgacacttca
     3361 cgggctatcg catcgagctg caggcttgca accaggacac ccctgaggaa cggtgcagtg
     3421 tggcagccta cgtcagtgcg aggaccatgc ctgaagccaa ggctgatgac attgttggcc
     3481 ctgtgacgca tgaaatcttt gagaacaacg tcgtccactt gatgtggcag gagccgaagg
     3541 agcccaatgg tctgatcgtg ctgtatgaag tgagttatcg gcgatatggt gatgaggagc
     3601 tgcatctctg cgtctcccgc aagcacttcg ctctggaacg gggctgcagg ctgcgtgggc
     3661 tgtcaccggg gaactacagc gtgcgaatcc gggccacctc ccttgcgggc aacggctctt
     3721 ggacggaacc cacctatttc tacgtgacag actatttaga cgtcccgtca aatattgcaa
     3781 aaattatcat cggccccctc atctttgtct ttctcttcag tgttgtgatt ggaagtattt
     3841 atctattcct gagaaagagg cagccagatg ggccgctggg accgctttac gcttcttcaa
     3901 accctgagta tctcagtgcc agtgatgtgt ttccatgctc tgtgtacgtg ccggacgagt
     3961 gggaggtgtc tcgagagaag atcaccctcc ttcgagagct ggggcagggc tccttcggca
     4021 tggtgtatga gggcaatgcc agggacatca tcaagggtga ggcagagacc cgcgtggcgg
     4081 tgaagacggt caacgagtca gccagtctcc gagagcggat tgagttcctc aatgaggcct
     4141 cggtcatgaa gggcttcacc tgccatcacg tggtgcgcct cctgggagtg gtgtccaagg
     4201 gccagcccac gctggtggtg atggagctga tggctcacgg agacctgaag agctacctcc
     4261 gttctctgcg gccagaggct gagaataatc ctggccgccc tccccctacc cttcaagaga
     4321 tgattcagat ggcggcagag attgctgacg ggatggccta cctgaacgcc aagaagtttg
     4381 tgcatcggga cctggcagcg agaaactgca tggtcgccca tgattttact gtcaaaattg
     4441 gagactttgg aatgaccaga gacatctatg aaacggatta ctaccggaaa gggggcaagg
     4501 gtctgctccc tgtacggtgg atggcaccgg agtccctgaa ggatggggtc ttcaccactt
     4561 cttctgacat gtggtccttt ggcgtggtcc tttgggaaat caccagcttg gcagaacagc
     4621 cttaccaagg cctgtctaat gaacaggtgt tgaaatttgt catggatgga gggtatctgg
     4681 atcaacccga caactgtcca gagagagtca ctgacctcat gcgcatgtgc tggcaattca
     4741 accccaagat gaggccaacc ttcctggaga ttgtcaacct gctcaaggac gacctgcacc
     4801 ccagctttcc agaggtgtcg ttcttccaca gcgaggagaa caaggctccc gagagtgagg
     4861 agctggagat ggagtttgag gacatggaga atgtgcccct ggaccgttcc tcgcactgtc
     4921 agagggagga ggcggggggc cgggatggag ggtcctcgct gggtttcaag cggagctacg
     4981 aggaacacat cccttacaca cacatgaacg gaggcaagaa aaacgggcgg attctgacct
     5041 tgcctcggtc caatccttcc aagggtcaag acaattctgc agatatccag cacagtggcg
     5101 gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
     5161 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
     5221 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5281 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5341 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5401 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     5461 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     5521 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5581 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5641 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5701 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5761 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5821 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     5881 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     5941 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6001 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6061 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6121 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6181 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     6241 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     6301 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     6361 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     6421 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     6481 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     6541 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     6601 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6661 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6721 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6781 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6841 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     6901 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6961 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     7021 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     7081 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     7141 ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     7201 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7261 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7321 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7381 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7441 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7501 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7561 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7621 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7681 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7741 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7801 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7861 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7921 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7981 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8041 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8101 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8161 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8221 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8281 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8341 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8401 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8461 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8521 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8581 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8641 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8701 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8761 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8821 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8881 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8941 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9001 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9061 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9121 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9181 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9241 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9301 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9361 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9421 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9481 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9541 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9601 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatc
//
