LOCUS       sequence-396817-        9713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human RON receptor tyrosine kinase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-RON-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(51..70)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        242..621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        622..825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     776..796
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     870..889
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        870..888
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5218..5259
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5269..5286
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5309..5326)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5315..5539
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5585..6013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5672..5691)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5882..5903
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6022..6042)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6027..6356
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6207..6342
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6269..6288
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6423..7217
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6477..6496)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7087..7106
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7393..7514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7430..7449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7484..7503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7563..7579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7563..7579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7576..7598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7587..7603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7611..7617,7618..7635,7636..7641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7656..7677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7794..7811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7965..8553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8045..8064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8724..9515,9516..9584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9347..9366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9585..9689)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
       61 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
      121 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
      181 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      241 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      601 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      661 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      721 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      781 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
      841 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
      901 ggctagttaa gcttggtacc gagctcggat ccagtaccct tcaccatgga gctcctcccg
      961 ccgctgcctc agtccttcct gttgctgctg ctgttgcctg ccaagcccgc ggcgggcgag
     1021 gactggcagt gcccgcgcac cccctacgcg gcctctcgcg actttgacgt gaagtacgtg
     1081 gtgcccagct tctccgccgg aggcctggta caggccatgg tgacctacga gggcgacaga
     1141 aatgagagtg ctgtgtttgt agccatacgc aatcgcctgc atgtgcttgg gcctgacctg
     1201 aagtctgtcc agagcctggc cacgggccct gctggagacc ctggctgcca gacgtgtgca
     1261 gcctgtggcc caggacccca cggccctccc ggtgacacag acacaaaggt gctggtgctg
     1321 gatcccgcgc tgcctgcgct ggtcagttgt ggctccagcc tgcagggccg ctgcttcctg
     1381 catgacctag agccccaagg gacagccgtg catctggcag cgccagcctg cctcttctca
     1441 gcccaccata accggcccga tgactgcccc gactgtgtgg ccagcccatt gggcacccgt
     1501 gtaactgtgg ttgagcaagg ccaggcctcc tatttctacg tggcatcctc actggacgca
     1561 gccgtggctg ccagcttcag cccacgctca gtgtctatca ggcgtctcaa ggctgacgcc
     1621 tcgggattcg caccgggctt tgtggcgttg tcagtgctgc ccaagcatct tgtctcctac
     1681 agtattgaat acgtgcacag cttccacacg ggagccttcg tatacttcct gactgtacag
     1741 ccggccagcg tgacagatga tcctagtgcc ctgcacacac gcctggcacg gcttagcgcc
     1801 actgagccag agttgggcga ctatcgggag ctggtcctcg actgcagatt tgctccaaaa
     1861 cgcaggcgcc ggggggcccc agaaggcgga cagccctacc ctgtgctgcg ggtggcccac
     1921 tccgctccag tgggtgccca acttgccact gagctgagca tcgccgaggg ccaggaagta
     1981 ctatttgggg tctttgtgac tggcaaggat ggtggtcctg gcgtgggccc caactctgtc
     2041 gtctgtgcct tccccattga cctgctggac acactaattg atgagggtgt ggagcgctgt
     2101 tgtgaatccc cagtccatcc aggcctccgg cgaggcctcg acttcttcca gtcgcccagt
     2161 ttttgcccca acccgcctgg cctggaagcc ctcagcccca acaccagctg ccgccacttc
     2221 cctctgctgg tcagtagcag cttctcacgt gtggacctat tcaatgggct gttgggacca
     2281 gtacaggtca ctgcattgta tgtgacacgc cttgacaacg tcacagtggc acacatgggc
     2341 acaatggatg ggcgtatcct gcaggtggag ctggtcaggt cactaaacta cttgctgtat
     2401 gtgtccaact tctcactggg tgacagtggg cagcccgtgc agcgggatgt cagtcgtctt
     2461 ggggaccacc tactctttgc ctctggggac caggttttcc aggtacctat ccaaggccct
     2521 ggctgccgcc acttcctgac ctgtgggcgt tgcctaaggg catggcattt catgggctgt
     2581 ggctggtgtg ggaacatgtg cggccagcag aaggagtgtc ctggctcctg gcaacaggac
     2641 cactgcccac ctaagcttac tgagttccac ccccacagtg gacctctaag gggcagtaca
     2701 aggctgaccc tgtgtggctc caacttctac cttcaccctt ctggtctggt gcctgaggga
     2761 acccatcagg tcactgtggg ccaaagtccc tgccggccac tgcccaagga cagctcaaaa
     2821 ctcagaccag tgccccggaa agactttgta gaggagtttg agtgtgaact ggagcccttg
     2881 ggcacccagg cagtggggcc taccaacgtc agcctcaccg tgactaacat gccaccgggc
     2941 aagcacttcc gggtagacgg cacctccgtg ctgagaggct tctctttcat ggagccagtg
     3001 ctgatagcag tgcaacccct ctttggccca cgggcaggag gcacctgtct cactcttgaa
     3061 ggccagagtc tgtctgtagg caccagccgg gctgtgctgg tcaatgggac tgagtgtctg
     3121 ctagcacggg tcagtgaggg gcagctttta tgtgccacac cccctggggc cacggtggcc
     3181 agtgtccccc ttagcctgca ggtggggggt gcccaggtac ctggttcctg gaccttccag
     3241 tacagagaag accctgtcgt gctaagcatc agccccaact gtggctacat caactcccac
     3301 atcaccatct gtggccagca tctaacttca gcatggcact tagtgctgtc attccatgac
     3361 gggcttaggg cagtggaaag caggtgtgag aggcagcttc cagagcagca gctgtgccgc
     3421 cttcctgaat atgtggtccg agacccccag ggatgggtgg cagggaatct gagtgcccga
     3481 ggggatggag ctgctggctt tacactgcct ggctttcgct tcctaccccc accccatcca
     3541 cccagtgcca acctagttcc actgaagcct gaggagcatg ccattaagtt tgagtatatt
     3601 gggctgggcg ctgtggctga ctgtgtgggt atcaacgtga ccgtgggtgg tgagagctgc
     3661 cagcacgagt tccgggggga catggttgtc tgccccctgc ccccatccct gcagcttggc
     3721 caggatggtg ccccattgca ggtctgcgta gatggtgaat gtcatatcct gggtagagtg
     3781 gtgcggccag ggccagatgg ggtcccacag agcacgctcc ttggtatcct gctgcctttg
     3841 ctgctgcttg tggctgcact ggcgactgca ctggtcttca gctactggtg gcggaggaag
     3901 cagctagttc ttcctcccaa cctgaatgac ctggcatccc tggaccagac tgctggagcc
     3961 acacccctgc ctattctgta ctcgggctct gactacagaa gtggccttgc actccctgcc
     4021 attgatggtc tggattccac cacttgtgtc catggagcat ccttctccga tagtgaagat
     4081 gaatcctgtg tgccactgct gcggaaagag tccatccagc taagggacct ggactctgcg
     4141 ctcttggctg aggtcaagga tgtgctgatt ccccatgagc gggtggtcac ccacagtgac
     4201 cgagtcattg gcaaaggcca ctttggagtt gtctaccacg gagaatacat agaccaggcc
     4261 cagaatcgaa tccaatgtgc catcaagtca ctaagtcgca tcacagagat gcagcaggtg
     4321 gaggccttcc tgcgagaggg gctgctcatg cgtggcctga accacccgaa tgtgctggct
     4381 ctcattggta tcatgttgcc acctgagggc ctgccccatg tgctgctgcc ctatatgtgc
     4441 cacggtgacc tgctccagtt catccgctca cctcagcgga accccaccgt gaaggacctc
     4501 atcagctttg gcctgcaggt agcccgcggc atggagtacc tggcagagca gaagtttgtg
     4561 cacagggacc tggctgcgcg gaactgcatg ctggacgagt cattcacagt caaggtggct
     4621 gactttggtt tggcccgcga catcctggac agggagtact atagtgttca acagcatcgc
     4681 cacgctcgcc tacctgtgaa gtggatggcg ctggagagcc tgcagaccta tagatttacc
     4741 accaagtctg atgtgtggtc atttggtgtg ctgctgtggg aactgctgac acggggtgcc
     4801 ccaccatacc gccacattga cccttttgac cttacccact tcctggccca gggtcggcgc
     4861 ctgccccagc ctgagtattg ccctgattct ctgtaccaag tgatgcagca atgctgggag
     4921 gcagacccag cagtgcgacc caccttcaga gtactagtgg gggaggtgga gcagatagtg
     4981 tctgcactgc ttggggacca ttatgtgcag ctgccagcaa cctacatgaa cttgggcccc
     5041 agcacctccc atgagatgaa tgtgcgtcca gaacagccgc agttctcacc catgccaggg
     5101 aatgtacgcc ggccccggcc actctcagag cctcctcggc ccactaaggg tcaagacaat
     5161 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     5221 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
     5281 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5341 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5401 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5461 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5521 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     5581 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5641 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5701 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5761 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5821 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5881 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5941 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6001 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6061 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6121 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6181 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6241 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6301 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6361 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     6421 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6481 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6541 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6601 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6661 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6721 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6781 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6841 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6901 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     6961 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7021 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7081 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7141 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7201 ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc
     7261 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     7321 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     7381 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7441 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7501 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     7561 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7621 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7681 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7741 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7801 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7861 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7921 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7981 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8041 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8101 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8161 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8221 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8281 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8341 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8401 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8461 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8521 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8581 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8641 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8701 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8761 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8821 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8881 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8941 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9001 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9061 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9121 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9181 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9241 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9301 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9361 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9421 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9481 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9541 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9601 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9661 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acc
//
